ID   AGO4_CHICK              Reviewed;         794 AA.
AC   Q5ZMW0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Protein argonaute-4 {ECO:0000255|HAMAP-Rule:MF_03033};
DE            Short=Argonaute4 {ECO:0000255|HAMAP-Rule:MF_03033};
DE   AltName: Full=Argonaute RISC catalytic component 4;
DE   AltName: Full=Eukaryotic translation initiation factor 2C 4 {ECO:0000255|HAMAP-Rule:MF_03033};
DE            Short=eIF-2C 4 {ECO:0000255|HAMAP-Rule:MF_03033};
DE            Short=eIF2C 4 {ECO:0000255|HAMAP-Rule:MF_03033};
GN   Name=AGO4; Synonyms=EIF2C4; ORFNames=RCJMB04_1a17;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to
CC       short RNAs such as microRNAs (miRNAs) and represses the translation of
CC       mRNAs which are complementary to them. Lacks endonuclease activity and
CC       does not appear to cleave target mRNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_03033}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000255|HAMAP-
CC       Rule:MF_03033}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03033}.
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DR   EMBL; AJ719274; CAG30933.1; -; mRNA.
DR   RefSeq; NP_001034365.1; NM_001039276.1.
DR   AlphaFoldDB; Q5ZMW0; -.
DR   SMR; Q5ZMW0; -.
DR   PaxDb; Q5ZMW0; -.
DR   Ensembl; ENSGALT00000049753; ENSGALP00000047611; ENSGALG00000039713.
DR   GeneID; 419629; -.
DR   KEGG; gga:419629; -.
DR   CTD; 192670; -.
DR   VEuPathDB; HostDB:geneid_419629; -.
DR   GeneTree; ENSGT00940000158729; -.
DR   InParanoid; Q5ZMW0; -.
DR   OMA; VGANRHY; -.
DR   OrthoDB; 159407at2759; -.
DR   PhylomeDB; Q5ZMW0; -.
DR   PRO; PR:Q5ZMW0; -.
DR   Proteomes; UP000000539; Chromosome 23.
DR   Bgee; ENSGALG00000039713; Expressed in spleen and 12 other tissues.
DR   ExpressionAtlas; Q5ZMW0; baseline and differential.
DR   GO; GO:0000932; C:P-body; IEA:UniProtKB-SubCell.
DR   GO; GO:0016442; C:RISC complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0035198; F:miRNA binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR   GO; GO:0035278; P:miRNA-mediated gene silencing by inhibition of translation; ISS:UniProtKB.
DR   GO; GO:0006402; P:mRNA catabolic process; ISS:UniProtKB.
DR   CDD; cd04657; Piwi_ago-like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_03033; AGO4; 1.
DR   InterPro; IPR028604; AGO4.
DR   InterPro; IPR014811; ArgoL1.
DR   InterPro; IPR032472; ArgoL2.
DR   InterPro; IPR032473; Argonaute_Mid_dom.
DR   InterPro; IPR032474; Argonaute_N.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR045246; Piwi_ago-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF08699; ArgoL1; 1.
DR   Pfam; PF16488; ArgoL2; 1.
DR   Pfam; PF16487; ArgoMid; 1.
DR   Pfam; PF16486; ArgoN; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM01163; DUF1785; 1.
DR   SMART; SM00949; PAZ; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Ribonucleoprotein; RNA-binding;
KW   RNA-mediated gene silencing; Translation regulation.
FT   CHAIN           1..794
FT                   /note="Protein argonaute-4"
FT                   /id="PRO_0000371225"
FT   DOMAIN          158..271
FT                   /note="PAZ"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03033"
FT   DOMAIN          442..753
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03033"
FT   REGION          758..779
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   794 AA;  89531 MW;  C97D19B3C096F554 CRC64;
     MVRHFKMQIF GDRQPGYDGK RNMYTAHPLP IGRDRVDMEV TLPGEGKDQT FKVSIQWVSV
     VSLQLLLEAL AGHLNEVPED SVQALDVITR HLPSMRYTPV GRSFFSPPEG YYHPLGGGRE
     VWFGFHQSVR PAMWNMMLNI DVSATAFYRA QPIIEFMCEV LDIQNINEQT KPLTDSQRVK
     FTKEIRGLKV EVTHCGQMKR KYRVCNVTRR PASHQTFPLQ LENGQAMECT VAQYFKQKYS
     LQLKYPHLPC LQVGQEQKHT YLPLEVCNIV AGQRCIKKLT DNQTSTMIKA TARSAPDRQE
     EISRLVKSNS MVGGPDPYLK EFGIVVHNEM TELTGRVLPA PMLQYGGRNK TVATPNQGVW
     DMRGKQFYAG IEIKVWAVAC FAPQKQCRED LLKSFTDQLR KISKDAGMPI QGQPCFCKYA
     QGADSVEPMF KHLKLTYVGL QLIVVILPGK TPVYAEVKRV GDTLLGMATQ CVQVKNVVKT
     SPQTLSNLCL KINAKLGGIN NVLVPHQRPS VFQQPVIFLG ADVTHPPAGD GKKPSIAAVV
     GSMDGHPSRY CATVRVQTSR QETSQELLYS QEVIQDLTNM VRELLIQFYK STRFKPTRII
     YYRGGVSEGQ MKQVAWPELI AIRKACISLE EDYRPGITYI VVQKRHHTRL FCADKTERVG
     KSGNVPAGTT VDSTITHPSE FDFYLCSHAG IQGTSRPSHY QVLWDDNCFT ADELQLLTYQ
     LCHTYVRCTR SVSIPAPAYY ARLVAFRARY HLVDKDHDSA EGSHVSGQSN GRDPQALAKA
     VQIHHDTQHT MYFA