ID   EPTA_ECO57              Reviewed;         547 AA.
AC   A0A0H3JML2;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2018, sequence version 2.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Phosphoethanolamine transferase EptA;
DE            EC=2.7.-.- {ECO:0000305|PubMed:16514146};
DE   AltName: Full=Polymyxin resistance protein PmrC;
GN   Name=eptA; Synonyms=pmrC; OrderedLocusNames=ECs_5096;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=O157:H7 / 4304, and O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=16514146; DOI=10.1099/mic.0.28692-0;
RA   Kim S.H., Jia W., Parreira V.R., Bishop R.E., Gyles C.L.;
RT   "Phosphoethanolamine substitution in the lipid A of Escherichia coli
RT   O157:H7 and its association with PmrC.";
RL   Microbiology 152:657-666(2006).
CC   -!- FUNCTION: There are several lipid A forms in this strain, including a
CC       phosphoethanolamine (1-O-P-pEtN) form; overexpression of this gene
CC       leads to higher levels of the 1-O-P-pEtN form of lipid A.
CC       {ECO:0000269|PubMed:16514146}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P30845}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Decreased amounts of the 1-O-P-PEtN form of lipid
CC       A. {ECO:0000269|PubMed:16514146}.
CC   -!- SIMILARITY: Belongs to the phosphoethanolamine transferase family. EptA
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BA000007; BAB38519.2; -; Genomic_DNA.
DR   RefSeq; NP_313123.2; NC_002695.1.
DR   RefSeq; WP_001302789.1; NZ_SDVX01000004.1.
DR   AlphaFoldDB; A0A0H3JML2; -.
DR   SMR; A0A0H3JML2; -.
DR   STRING; 155864.EDL933_5459; -.
DR   EnsemblBacteria; BAB38519; BAB38519; ECs_5096.
DR   GeneID; 914018; -.
DR   KEGG; ecs:ECs_5096; -.
DR   PATRIC; fig|386585.9.peg.5326; -.
DR   eggNOG; COG2194; Bacteria.
DR   HOGENOM; CLU_018534_1_0_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008484; F:sulfuric ester hydrolase activity; IEA:InterPro.
DR   GO; GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IEA:InterPro.
DR   Gene3D; 3.40.720.10; -; 1.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR012549; EptA-like_N.
DR   InterPro; IPR040423; PEA_transferase.
DR   InterPro; IPR000917; Sulfatase_N.
DR   PANTHER; PTHR30443; PTHR30443; 1.
DR   Pfam; PF08019; EptA_B_N; 1.
DR   Pfam; PF00884; Sulfatase; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..547
FT                   /note="Phosphoethanolamine transferase EptA"
FT                   /id="PRO_0000446241"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   547 AA;  61766 MW;  5A80405B0BF4FD0D CRC64;
     MLKRLLKRPS LNLLAWLLLA AFYISICLNI AFFKQVLQAL PLDSLHNVLV FLSMPVVAFS
     VINIVLTLSS FLWLNRPLAC LFILVGAAAQ YFIMTYGIVI DRSMIANIID TTPAESYALM
     TPQMLLTLGF SGVLAALIAC WIKIKPATSR LRSVLFRGAN ILVSVLLILL VAALFYKDYA
     SLFRNNKELV KSLSPSNSIV ASWSWYSHQR LANLPLVRIG EDAHRNPLMQ NEKRKNLTIL
     IVGETSRAEN FSLNGYPRET NPRLAKDNVV YFPNTASCGT ATAVSVPCMF SDMPREHYKE
     ELAQHQEGVL DIIQRAGINV LWNDNDGGCK GACDRVPHQN VTALNLPDQC INGECYDEVL
     FHGLEEYINN LQGDGVIVLH TIGSHRPTYY NRYPPQFRKF TPTCDTNEIQ TCTKEQLVNT
     YDNTLVYVDY IVDKAINLLK EHQDKFTTSL VYLSDHGESL GENGIYLHGL PYAIAPDSQK
     QVPMLLWLSE DYQKRYQVDQ NCLQKQAQTQ HYSQDNLFST LLGLTGVETK YYQAADDILQ
     TCRRVSE