ID   AGO6_ARATH              Reviewed;         878 AA.
AC   O48771;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Protein argonaute 6;
GN   Name=AGO6; OrderedLocusNames=At2g32940; ORFNames=T21L14.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION.
RX   PubMed=17869110; DOI=10.1016/j.cub.2007.08.039;
RA   Baumberger N., Tsai C.-H., Lie M., Havecker E., Baulcombe D.C.;
RT   "The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for
RT   degradation.";
RL   Curr. Biol. 17:1609-1614(2007).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=17332757; DOI=10.1038/sj.emboj.7601603;
RA   Zheng X., Zhu J., Kapoor A., Zhu J.K.;
RT   "Role of Arabidopsis AGO6 in siRNA accumulation, DNA methylation and
RT   transcriptional gene silencing.";
RL   EMBO J. 26:1691-1701(2007).
RN   [5]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=20173091; DOI=10.1105/tpc.109.072199;
RA   Havecker E.R., Wallbridge L.M., Hardcastle T.J., Bush M.S., Kelly K.A.,
RA   Dunn R.M., Schwach F., Doonan J.H., Baulcombe D.C.;
RT   "The Arabidopsis RNA-directed DNA methylation argonautes functionally
RT   diverge based on their expression and interaction with target loci.";
RL   Plant Cell 22:321-334(2010).
CC   -!- FUNCTION: Involved in transcriptional gene silencing (TGS). Component
CC       of the RISC complex that associate with the small interfering RNA
CC       (siRNA) pathway involved in direct cytosine methylation at endogenous
CC       DNA repeats. Required for the accumulation of specific siRNAs derived
CC       from transgene and heterochromatin-related endogenous loci. Involved in
CC       RNA-directed DNA methylation (RdDM) at specific endogenous loci.
CC       Probably not required for the accumulation of siRNAs derived from
CC       transgene inverted repeats that induce post-transcriptional gene
CC       silencing (PTGS). Associates mainly with small RNAs of 24 nucleotide in
CC       length and preferentially recruits small RNAs with a 5' terminal
CC       adenosine. Targeted by turnip yellows virus (TuYV) protein P0 (via F-
CC       box-like domain) for probable proteasome degradation and thereby
CC       inactivating AGO6 function in RNA silencing.
CC       {ECO:0000269|PubMed:17332757, ECO:0000269|PubMed:17869110,
CC       ECO:0000269|PubMed:20173091}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17332757}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons and shoot
CC       meristematic region. {ECO:0000269|PubMed:17332757,
CC       ECO:0000269|PubMed:20173091}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB91987.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC003033; AAB91987.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC08765.1; -; Genomic_DNA.
DR   PIR; T01113; T01113.
DR   RefSeq; NP_180853.2; NM_128854.4.
DR   AlphaFoldDB; O48771; -.
DR   SMR; O48771; -.
DR   STRING; 3702.AT2G32940.1; -.
DR   PaxDb; O48771; -.
DR   PRIDE; O48771; -.
DR   ProteomicsDB; 244721; -.
DR   EnsemblPlants; AT2G32940.1; AT2G32940.1; AT2G32940.
DR   GeneID; 817856; -.
DR   Gramene; AT2G32940.1; AT2G32940.1; AT2G32940.
DR   KEGG; ath:AT2G32940; -.
DR   Araport; AT2G32940; -.
DR   TAIR; locus:2059370; AT2G32940.
DR   eggNOG; KOG1041; Eukaryota.
DR   HOGENOM; CLU_004544_2_0_1; -.
DR   InParanoid; O48771; -.
DR   OMA; MNDQYYT; -.
DR   OrthoDB; 220258at2759; -.
DR   PhylomeDB; O48771; -.
DR   PRO; PR:O48771; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O48771; baseline and differential.
DR   Genevisible; O48771; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0035197; F:siRNA binding; IDA:TAIR.
DR   GO; GO:0051607; P:defense response to virus; IDA:TAIR.
DR   GO; GO:0006306; P:DNA methylation; IMP:TAIR.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0030422; P:siRNA processing; IMP:TAIR.
DR   CDD; cd04657; Piwi_ago-like; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR014811; ArgoL1.
DR   InterPro; IPR032472; ArgoL2.
DR   InterPro; IPR032474; Argonaute_N.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR045246; Piwi_ago-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF08699; ArgoL1; 1.
DR   Pfam; PF16488; ArgoL2; 1.
DR   Pfam; PF16486; ArgoN; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM01163; DUF1785; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Plant defense; Reference proteome; Repressor; Ribonucleoprotein;
KW   RNA-binding; RNA-mediated gene silencing; Transcription;
KW   Transcription regulation; Translation regulation.
FT   CHAIN           1..878
FT                   /note="Protein argonaute 6"
FT                   /id="PRO_0000404668"
FT   DOMAIN          256..372
FT                   /note="PAZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT   DOMAIN          541..851
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   878 AA;  98681 MW;  6EF8E441BD65D5E5 CRC64;
     METSSSLPLS PISIEPEQPS HRDYDITTRR GVGTTGNPIE LCTNHFNVSV RQPDVVFYQY
     TVSITTENGD AVDGTGISRK LMDQLFKTYS SDLDGKRLAY DGEKTLYTVG PLPQNEFDFL
     VIVEGSFSKR DCGVSDGGSS SGTCKRSKRS FLPRSYKVQI HYAAEIPLKT VLGTQRGAYT
     PDKSAQDALR VLDIVLRQQA AERGCLLVRQ AFFHSDGHPM KVGGGVIGIR GLHSSFRPTH
     GGLSLNIDVS TTMILEPGPV IEFLKANQSV ETPRQIDWIK AAKMLKHMRV KATHRNMEFK
     IIGLSSKPCN QQLFSMKIKD GEREVPIREI TVYDYFKQTY TEPISSAYFP CLDVGKPDRP
     NYLPLEFCNL VSLQRYTKPL SGRQRVLLVE SSRQKPLERI KTLNDAMHTY CYDKDPFLAG
     CGISIEKEMT QVEGRVLKPP MLKFGKNEDF QPCNGRWNFN NKMLLEPRAI KSWAIVNFSF
     PCDSSHISRE LISCGMRKGI EIDRPFALVE EDPQYKKAGP VERVEKMIAT MKLKFPDPPH
     FILCILPERK TSDIYGPWKK ICLTEEGIHT QCICPIKISD QYLTNVLLKI NSKLGGINSL
     LGIEYSYNIP LINKIPTLIL GMDVSHGPPG RADVPSVAAV VGSKCWPLIS RYRAAVRTQS
     PRLEMIDSLF QPIENTEKGD NGIMNELFVE FYRTSRARKP KQIIIFRDGV SESQFEQVLK
     IEVDQIIKAY QRLGESDVPK FTVIVAQKNH HTKLFQAKGP ENVPAGTVVD TKIVHPTNYD
     FYMCAHAGKI GTSRPAHYHV LLDEIGFSPD DLQNLIHSLS YVNQRSTTAT SIVAPVRYAH
     LAAAQVAQFT KFEGISEDGK VPELPRLHEN VEGNMFFC