ERA1_MAIZE
ID ERA1_MAIZE Reviewed; 416 AA.
AC K7UTH7; B4FIL9;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2013, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=GTPase ERA1, chloroplastic {ECO:0000305};
DE Short=Zm-ERA1 {ECO:0000303|PubMed:25725436};
DE AltName: Full=GTP-binding protein ERA1 {ECO:0000305};
DE Flags: Precursor;
GN Name=ERA1 {ECO:0000303|PubMed:25725436};
GN ORFNames=ZEAMMB73_Zm00001d009596 {ECO:0000312|EMBL:AQK92220.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=22065420; DOI=10.1104/pp.111.188474;
RA Majeran W., Friso G., Asakura Y., Qu X., Huang M., Ponnala L.,
RA Watkins K.P., Barkan A., van Wijk K.J.;
RT "Nucleoid-enriched proteomes in developing plastids and chloroplasts from
RT maize leaves: a new conceptual framework for nucleoid functions.";
RL Plant Physiol. 158:156-189(2012).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25725436; DOI=10.1016/j.bbabio.2015.02.014;
RA Belcher S., Williams-Carrier R., Stiffler N., Barkan A.;
RT "Large-scale genetic analysis of chloroplast biogenesis in maize.";
RL Biochim. Biophys. Acta 1847:1004-1016(2015).
CC -!- FUNCTION: Nuclear genome-encoded probable GTPase involved in ribosome
CC biogenesis in chloroplasts. Plays a role in 16S rRNA maturation in
CC plastids and may contribute to the assembly of the small (30S)
CC ribosomal subunit. {ECO:0000269|PubMed:25725436}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid
CC {ECO:0000269|PubMed:22065420}.
CC -!- DISRUPTION PHENOTYPE: Very pale yellow green leaf phenotype. Virescent
CC leaf phenotype. {ECO:0000269|PubMed:25725436}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. Era GTPase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01050, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACF81962.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ACG35633.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; CM000784; AQK92220.1; -; Genomic_DNA.
DR EMBL; EU963515; ACG35633.1; ALT_FRAME; mRNA.
DR EMBL; BT036957; ACF81962.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001136454.1; NM_001142982.1.
DR RefSeq; XP_008654454.1; XM_008656232.1.
DR AlphaFoldDB; K7UTH7; -.
DR SMR; K7UTH7; -.
DR STRING; 4577.GRMZM2G158024_P02; -.
DR PaxDb; K7UTH7; -.
DR PRIDE; K7UTH7; -.
DR EnsemblPlants; Zm00001eb343970_T002; Zm00001eb343970_P002; Zm00001eb343970.
DR GeneID; 100216562; -.
DR Gramene; Zm00001eb343970_T002; Zm00001eb343970_P002; Zm00001eb343970.
DR eggNOG; KOG1423; Eukaryota.
DR HOGENOM; CLU_038009_1_3_1; -.
DR OMA; WAEVDVI; -.
DR OrthoDB; 586738at2759; -.
DR Proteomes; UP000007305; Chromosome 8.
DR ExpressionAtlas; K7UTH7; baseline and differential.
DR GO; GO:0042644; C:chloroplast nucleoid; IDA:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR GO; GO:0042254; P:ribosome biogenesis; IMP:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IMP:UniProtKB.
DR CDD; cd04163; Era; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00367; GTPase_Era; 1.
DR InterPro; IPR030388; G_ERA_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR005662; GTP-bd_Era.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR004044; KH_dom_type_2.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR PANTHER; PTHR42698; PTHR42698; 1.
DR Pfam; PF07650; KH_2; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54814; SSF54814; 1.
DR TIGRFAMs; TIGR00436; era; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51713; G_ERA; 1.
DR PROSITE; PS50823; KH_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; GTP-binding; Nucleotide-binding; Plastid; Reference proteome;
KW Ribosome biogenesis; RNA-binding; Transit peptide.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 54..416
FT /note="GTPase ERA1, chloroplastic"
FT /id="PRO_0000441334"
FT DOMAIN 117..287
FT /note="Era-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT DOMAIN 318..395
FT /note="KH type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00118"
FT REGION 125..132
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 151..155
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 172..175
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 237..240
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 266..268
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT BINDING 125..132
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 172..176
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 237..240
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
SQ SEQUENCE 416 AA; 46843 MW; 07DCD1022A5DF24E CRC64;
MELGLALRLV APPPLLPCLS RRALSLPPDF VSSRVLRGRR IHASRLKHGA GVVCNAIMTY
SGVEEEEMVE EEMEEEAEPA VSTRPRLELI EKPDRSLALL DEYESEELGT SLCANHRSGY
VAVLGKPNVG KSTLINQMVG QKLSIVTDKP QTTRHRILGI CSEPEYQIIL YDTPGVIKKE
MHKLDSMMMK NVRSAIGSAD CVLVVADACK TPEKIDEMLE EGVGNKDIGL PVLLVLNKKD
LIKPGEIAKK LEWYQKFTNV DDVIPISAKF GHGVDDIKEW ILSKLPLGPA YYPKDIASEH
PERFFVGEIV REKIFVQYRQ EIPYSCQVNV VSYKSRPAAK DFIQVEILVE KESQRGIILG
KDGKSIKMLA TASRLDIEDF LQKKVYLEVE VKVKENWRQD ERLLKRYGYG GEIQAL
