ID   AGO8_ARATH              Reviewed;         850 AA.
AC   Q3E984;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Protein argonaute 8;
GN   Name=AGO8; OrderedLocusNames=At5g21030; ORFNames=T10F18.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Involved in RNA-mediated post-transcriptional gene silencing
CC       (PTGS). Main component of the RNA-induced silencing complex (RISC) that
CC       binds to a short guide RNA such as a microRNA (miRNA) or small
CC       interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide
CC       for slicer-directed cleavage of homologous mRNAs to repress gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AC069325; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED92921.1; -; Genomic_DNA.
DR   RefSeq; NP_197602.2; NM_122111.3.
DR   AlphaFoldDB; Q3E984; -.
DR   SMR; Q3E984; -.
DR   STRING; 3702.AT5G21030.1; -.
DR   iPTMnet; Q3E984; -.
DR   PaxDb; Q3E984; -.
DR   PRIDE; Q3E984; -.
DR   EnsemblPlants; AT5G21030.1; AT5G21030.1; AT5G21030.
DR   GeneID; 832227; -.
DR   Gramene; AT5G21030.1; AT5G21030.1; AT5G21030.
DR   KEGG; ath:AT5G21030; -.
DR   Araport; AT5G21030; -.
DR   TAIR; locus:2147072; AT5G21030.
DR   eggNOG; KOG1041; Eukaryota.
DR   HOGENOM; CLU_004544_2_0_1; -.
DR   InParanoid; Q3E984; -.
DR   PhylomeDB; Q3E984; -.
DR   PRO; PR:Q3E984; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q3E984; baseline and differential.
DR   Genevisible; Q3E984; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProt.
DR   GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   CDD; cd04657; Piwi_ago-like; 1.
DR   Gene3D; 3.30.420.10; -; 2.
DR   InterPro; IPR014811; ArgoL1.
DR   InterPro; IPR032472; ArgoL2.
DR   InterPro; IPR032474; Argonaute_N.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR045246; Piwi_ago-like.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF08699; ArgoL1; 1.
DR   Pfam; PF16488; ArgoL2; 1.
DR   Pfam; PF16486; ArgoN; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM01163; DUF1785; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Repressor; Ribonucleoprotein; RNA-binding;
KW   RNA-mediated gene silencing; Transcription; Transcription regulation;
KW   Translation regulation.
FT   CHAIN           1..850
FT                   /note="Protein argonaute 8"
FT                   /id="PRO_0000404670"
FT   DOMAIN          241..361
FT                   /note="PAZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT   DOMAIN          518..811
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   850 AA;  95507 MW;  669EC27D7F4AC375 CRC64;
     MDTTLPPPQH MEREPLKSKS SLLPMTRRGN GSKGQKILLL TNHFRVNFRK PNSHNFFHYS
     VTITYEDGSP LLAKGFGRKI LEKVQQTCQA DLGCKHFAYD GDKNLYTVGP LPRSSLDFSV
     VLETAPSRRN ADKRLKLPHQ SKKFNVAILF APPEIPMEAI ANALQGKKTK HLLDAIRVMD
     CILSQNAARQ GCLLVRQSFF HNDAKYFANI GEGVDCCKGF HSSFRTTQGG LSLNIDVSTA
     MIVKPGPVVD FLIANQGVND PFSINWKKAK NTLKNLRVKV LPSNQEYKIT GLSGLHCKDQ
     TFTWKKRNQN REFEEVEITV SDYFTRIREI ELRYSGGLPC INVGKPNRPT YFPIELCELV
     SLQRYTKALT KFQRSNLIKE SRQNPQQRIG VLTRALKTSN YNDDPMLQEC GVRIGSDFTQ
     VEGRVLPTPK LKAGKEQDIY PINGSWNFKN KPATVTRWAV VNFSARCDPQ KIIDDLTRCG
     KMKGINVDSP YHVVFEENPQ FKDATGSVRV DKMFQHLQSI LGEVPPKFLL CILEKKNSDV
     YEKSCSMWNC ECIVPPQNLN DQYLTNLLLK INAKLGGLNS VLDMELSGTM PLVMRVPTII
     IGMDVSHGSP GQSDHIPSIA AVVSSREWPL ISKYRACVRT QSPKVEMIDS LFKPVSDKDD
     QGIMRELLLD FHSSSGKKPN HIIIFRDGVS ESQFNQVLNI ELDQMMQINH HTKFFQTESP
     NNVLPGTIID SNICHQHNND FYLCAHAGKI GTTRPTHYHV LYDEIGFDTD QLQELVHSLS
     YVYQRSTTAI SLVAPICYAH LAAAQMATAM KFEDMSETSS SHGGITTAGA VPVPPMPKLN
     TNVASSMFFC