AGO9_ARATH
ID AGO9_ARATH Reviewed; 896 AA.
AC Q84VQ0; Q56X15; Q84YI4;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein argonaute 9;
GN Name=AGO9; OrderedLocusNames=At5g21150; ORFNames=T10F18.180;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta; TISSUE=Anther, and Ovule;
RX PubMed=12805595; DOI=10.1104/pp.102.017798;
RA Scutt C.P., Vinauger-Douard M., Fourquin C., Ailhas J., Kuno N., Uchida K.,
RA Gaude T., Furuya M., Dumas C.;
RT "The identification of candidate genes for a reverse genetic analysis of
RT development and function in the Arabidopsis gynoecium.";
RL Plant Physiol. 132:653-665(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 317-896.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=17869110; DOI=10.1016/j.cub.2007.08.039;
RA Baumberger N., Tsai C.-H., Lie M., Havecker E., Baulcombe D.C.;
RT "The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for
RT degradation.";
RL Curr. Biol. 17:1609-1614(2007).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20208518; DOI=10.1038/nature08828;
RA Olmedo-Monfil V., Duran-Figueroa N., Arteaga-Vazquez M., Demesa-Arevalo E.,
RA Autran D., Grimanelli D., Slotkin R.K., Martienssen R.A.,
RA Vielle-Calzada J.P.;
RT "Control of female gamete formation by a small RNA pathway in
RT Arabidopsis.";
RL Nature 464:628-632(2010).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20173091; DOI=10.1105/tpc.109.072199;
RA Havecker E.R., Wallbridge L.M., Hardcastle T.J., Bush M.S., Kelly K.A.,
RA Dunn R.M., Schwach F., Doonan J.H., Baulcombe D.C.;
RT "The Arabidopsis RNA-directed DNA methylation argonautes functionally
RT diverge based on their expression and interaction with target loci.";
RL Plant Cell 22:321-334(2010).
RN [8]
RP FUNCTION.
RX PubMed=21057207; DOI=10.4161/psb.5.11.13548;
RA Duran-Figueroa N., Vielle-Calzada J.P.;
RT "ARGONAUTE9-dependent silencing of transposable elements in pericentromeric
RT regions of Arabidopsis.";
RL Plant Signal. Behav. 5:1476-1479(2010).
CC -!- FUNCTION: Involved in RNA-mediated post-transcriptional gene silencing
CC (PTGS). Main component of the RNA-induced silencing complex (RISC) that
CC binds to a short guide RNA such as a microRNA (miRNA) or small
CC interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide
CC for slicer-directed cleavage of homologous mRNAs to repress gene
CC expression. Associates preferentially with small RNAs of 24 nucleotide
CC in length with a 5' terminal adenosine. Interacts with 24 nucleotide
CC sRNAs derived from transposable elements (TEs). Required to silence
CC pericentrometric-located TEs in female gametes and their accessory
CC cells. Necessary to inactivate a significant proportion of long
CC terminal repeat retrotransposons (LTRs) in the ovule. Required to
CC specify cell fate in ovule. Involved in the control of female gamete
CC formation by restricting the specification of gametophyte precursors in
CC a dosage-dependent, non-cell-autonomous manner. Targeted by turnip
CC yellows virus (TuYV) protein P0 (via F-box-like domain) for probable
CC proteasome degradation and thereby inactivating AGO9 function in RNA
CC silencing. {ECO:0000269|PubMed:17869110, ECO:0000269|PubMed:20173091,
CC ECO:0000269|PubMed:20208518, ECO:0000269|PubMed:21057207}.
CC -!- TISSUE SPECIFICITY: Expressed in embryonic shoot apex region, pollen
CC and developing ovules. {ECO:0000269|PubMed:20173091}.
CC -!- DEVELOPMENTAL STAGE: Expressed in all cells of the young ovule
CC primordium at pre-meiotic stages, including the L1 layer. At early
CC stages of female gametogenesis, expression is restricted to the distal
CC (micropylar) portion of the developing ovule, but is absent from the 1-
CC nuclear female gametophyte. In fully differentiated ovules, expressed
CC at the proximal and distal poles but not in the female gametophyte.
CC {ECO:0000269|PubMed:20208518}.
CC -!- DISRUPTION PHENOTYPE: Differentiation of multiple gametic cells able to
CC initiate gametogenesis. Abnormally enlarged sub-epidermal cells in
CC developing ovules. {ECO:0000269|PubMed:20208518}.
CC -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO73892.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ544236; CAD66636.1; -; mRNA.
DR EMBL; AC140977; AAO73892.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92940.1; -; Genomic_DNA.
DR EMBL; AK221864; BAD94152.1; -; mRNA.
DR RefSeq; NP_197613.2; NM_122122.3.
DR AlphaFoldDB; Q84VQ0; -.
DR SMR; Q84VQ0; -.
DR IntAct; Q84VQ0; 1.
DR STRING; 3702.AT5G21150.1; -.
DR PaxDb; Q84VQ0; -.
DR PRIDE; Q84VQ0; -.
DR ProteomicsDB; 244664; -.
DR EnsemblPlants; AT5G21150.1; AT5G21150.1; AT5G21150.
DR GeneID; 832241; -.
DR Gramene; AT5G21150.1; AT5G21150.1; AT5G21150.
DR KEGG; ath:AT5G21150; -.
DR Araport; AT5G21150; -.
DR TAIR; locus:2179008; AT5G21150.
DR eggNOG; KOG1041; Eukaryota.
DR HOGENOM; CLU_004544_2_0_1; -.
DR InParanoid; Q84VQ0; -.
DR OMA; FTHVEGR; -.
DR OrthoDB; 159407at2759; -.
DR PhylomeDB; Q84VQ0; -.
DR PRO; PR:Q84VQ0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q84VQ0; baseline and differential.
DR Genevisible; Q84VQ0; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0035197; F:siRNA binding; IDA:TAIR.
DR GO; GO:0051607; P:defense response to virus; IDA:TAIR.
DR GO; GO:1904159; P:megasporocyte differentiation; IMP:TAIR.
DR GO; GO:0009554; P:megasporogenesis; IMP:TAIR.
DR GO; GO:0010529; P:negative regulation of transposition; IMP:TAIR.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd04657; Piwi_ago-like; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR014811; ArgoL1.
DR InterPro; IPR032472; ArgoL2.
DR InterPro; IPR032474; Argonaute_N.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR045246; Piwi_ago-like.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF08699; ArgoL1; 1.
DR Pfam; PF16488; ArgoL2; 1.
DR Pfam; PF16486; ArgoN; 1.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM01163; DUF1785; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; SSF101690; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 2: Evidence at transcript level;
KW Plant defense; Reference proteome; Repressor; Ribonucleoprotein;
KW RNA-binding; RNA-mediated gene silencing; Transcription;
KW Transcription regulation; Translation regulation.
FT CHAIN 1..896
FT /note="Protein argonaute 9"
FT /id="PRO_0000404671"
FT DOMAIN 264..380
FT /note="PAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT DOMAIN 550..857
FT /note="Piwi"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT CONFLICT 268
FT /note="V -> I (in Ref. 1; CAD66636)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="R -> C (in Ref. 4; BAD94152)"
FT /evidence="ECO:0000305"
FT CONFLICT 571
FT /note="N -> D (in Ref. 1; CAD66636)"
FT /evidence="ECO:0000305"
FT CONFLICT 616
FT /note="P -> T (in Ref. 4; BAD94152)"
FT /evidence="ECO:0000305"
FT CONFLICT 827
FT /note="Y -> H (in Ref. 1; CAD66636)"
FT /evidence="ECO:0000305"
FT CONFLICT 871
FT /note="I -> M (in Ref. 4; BAD94152)"
FT /evidence="ECO:0000305"
FT CONFLICT 876
FT /note="A -> T (in Ref. 1; CAD66636)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 896 AA; 100524 MW; F2043904C1293300 CRC64;
MDSDEPNGSG LPPPPPFVPA NLVPEVEPVK KNILLPMARP RGSGSKGQKI PLLTNHFGVK
FNKPSGYFFH YSVAINYEDG RPVEAKGIGR KILDKVQETY QSDLGAKYFA YDGEKTLFTV
GALPSNKLDF SVVLEEIPSS RNHAGNDTND ADRKRSRRPN QTKKFMVEIS YAAKIPMQAI
ASALQGKETE NLQDALRVLD IILRQSAARQ GCLLVRQSFF HNDVKNFVPI GGGVSGCRGF
HSSFRTTQGG LSLNIDTSTT MIVQPGPVVD FLLANQNKKD PYGMDWNKAR RVLKNLRVQI
TLSNREYKIS GLSEHSCKDQ LFTWRKPNDK GEFEEVEITV LNYYKERNIE VRYSGDFPCI
NVGKPKRPTY FPIEFCNLVS LQRYTKSLTN FQRAALVEKS RQKPPERMAS LTKGLKDSNY
NADPVLQDSG VSIITNFTQV EGRILPTPML KVGKGENLSP IKGKWNFMRK TLAEPTTVTR
WAVVNFSARC DTNTLIRDLI KCGREKGINV EPPFKDVINE NPQFRNAPAT VRVENMFEQI
KSKLPKPPLF LLCILAERKN SDVYGPWKKK NLVDLGIVTQ CIAPTRLNDQ YLTNVLLKIN
AKLGGLNSLL AMERSPAMPK VTQVPTIIVG MDVSHGSPGQ SDIPSIAAVV SSRQWPLISK
YKACVRTQSR KMEMIDNLFK PVNGKDEGMF RELLLDFYYS SENRKPEHII IFRDGVSESQ
FNQVLNIELD QMMQACKFLD DTWHPKFTVI VAQKNHHTKF FQSRGPDNVP PGTIIDSQIC
HPRNFDFYLC AHAGMIGTTR PTHYHVLYDE IGFATDDLQE LVHSLSYVYQ RSTTAISVVA
PVCYAHLAAA QMGTVMKYEE LSETSSSHGG ITTPGAVPVP PMPQLHNNVS TSMFFC
