ERA_ARATH
ID ERA_ARATH Reviewed; 427 AA.
AC Q8VZ74; Q9FJZ2;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=GTPase ERA-like, chloroplastic {ECO:0000305};
DE AltName: Full=GTP-binding protein Era-like {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=At5g66470 {ECO:0000312|Araport:AT5G66470};
GN ORFNames=K1F13.13 {ECO:0000312|EMBL:AAL38371.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Nuclear genome-encoded probable GTPase involved in ribosome
CC biogenesis in chloroplasts. Plays a role in 16S rRNA maturation in
CC plastids and may contribute to the assembly of the small (30S)
CC ribosomal subunit. {ECO:0000250|UniProtKB:K7UTH7}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid
CC {ECO:0000250|UniProtKB:K7UTH7}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. Era GTPase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01050}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10923.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB013389; BAB10923.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98218.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71024.1; -; Genomic_DNA.
DR EMBL; AY065195; AAL38371.1; -; mRNA.
DR EMBL; AY093245; AAM13244.1; -; mRNA.
DR RefSeq; NP_001332584.1; NM_001345764.1.
DR RefSeq; NP_201448.2; NM_126045.6.
DR AlphaFoldDB; Q8VZ74; -.
DR SMR; Q8VZ74; -.
DR IntAct; Q8VZ74; 1.
DR STRING; 3702.AT5G66470.1; -.
DR iPTMnet; Q8VZ74; -.
DR PaxDb; Q8VZ74; -.
DR PRIDE; Q8VZ74; -.
DR ProteomicsDB; 222264; -.
DR EnsemblPlants; AT5G66470.1; AT5G66470.1; AT5G66470.
DR EnsemblPlants; AT5G66470.2; AT5G66470.2; AT5G66470.
DR GeneID; 836779; -.
DR Gramene; AT5G66470.1; AT5G66470.1; AT5G66470.
DR Gramene; AT5G66470.2; AT5G66470.2; AT5G66470.
DR KEGG; ath:AT5G66470; -.
DR Araport; AT5G66470; -.
DR TAIR; locus:2154915; AT5G66470.
DR eggNOG; KOG1423; Eukaryota.
DR HOGENOM; CLU_038009_1_3_1; -.
DR InParanoid; Q8VZ74; -.
DR OMA; WAEVDVI; -.
DR OrthoDB; 586738at2759; -.
DR PhylomeDB; Q8VZ74; -.
DR PRO; PR:Q8VZ74; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VZ74; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0042644; C:chloroplast nucleoid; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR GO; GO:0042254; P:ribosome biogenesis; ISS:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR CDD; cd04163; Era; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00367; GTPase_Era; 1.
DR InterPro; IPR030388; G_ERA_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR005662; GTP-bd_Era.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR004044; KH_dom_type_2.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR PANTHER; PTHR42698; PTHR42698; 1.
DR Pfam; PF07650; KH_2; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54814; SSF54814; 1.
DR TIGRFAMs; TIGR00436; era; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51713; G_ERA; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; GTP-binding; Nucleotide-binding; Plastid; Reference proteome;
KW Ribosome biogenesis; RNA-binding; Transit peptide.
FT TRANSIT 1..39
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 40..427
FT /note="GTPase ERA-like, chloroplastic"
FT /id="PRO_0000441335"
FT DOMAIN 128..298
FT /note="Era-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT DOMAIN 329..406
FT /note="KH type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00118"
FT REGION 136..143
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 162..166
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 183..186
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 248..251
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 277..279
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT BINDING 136..143
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 183..187
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 248..251
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
SQ SEQUENCE 427 AA; 48931 MW; 4BCEA02603A2A092 CRC64;
MAVSPHISPT LSRYKFFSTS VVENPNFSPY RIYSRRRVTK SHLQAHNSTT SYGRTELSSS
KKLWIRQRSF SEMEVEQAQL EDDEEQVEID IVDEASLLSL SMKPDRNMAL LDDYEMEELG
HTPNTHHRSG YVAVVGMPNV GKSTLSNQMI GQKISIVTDK PQTTRHRILG ICSSPEYQMI
LYDTPGVIEK KMHRLDTMMM KNVRDAAINA DCVVILVDAC KTPTNIEEVL KEGLGDLEKK
PPMLLVMNKK DLIKPGEIAK KLEWYEKFTD VDEVIPVSAK YGHGIEDVKE WILSKLPFGP
PYYPKDIVSE HPERFFVSEI VREKIFMQYR NEVPYACQVN VLSYKTRPAA KDFIQVEVVV
DKNSQKIILI GKEGKALKTL ATAARLDIED FLQKKVFLEV EVKVKENWRQ DEGLLKYYGY
GGQIRAM
