ID   AGO_GIAIC               Reviewed;         900 AA.
AC   A8BCK6;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Protein argonaute {ECO:0000250|UniProtKB:Q86QW7};
GN   Name=Ago {ECO:0000250|UniProtKB:Q86QW7}; ORFNames=GL50803_2902;
OS   Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=184922;
RN   [1] {ECO:0000312|EMBL:EDO80252.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 50803 / WB clone C6;
RX   PubMed=17901334; DOI=10.1126/science.1143837;
RA   Morrison H.G., McArthur A.G., Gillin F.D., Aley S.B., Adam R.D.,
RA   Olsen G.J., Best A.A., Cande W.Z., Chen F., Cipriano M.J., Davids B.J.,
RA   Dawson S.C., Elmendorf H.G., Hehl A.B., Holder M.E., Huse S.M., Kim U.U.,
RA   Lasek-Nesselquist E., Manning G., Nigam A., Nixon J.E.J., Palm D.,
RA   Passamaneck N.E., Prabhu A., Reich C.I., Reiner D.S., Samuelson J.,
RA   Svard S.G., Sogin M.L.;
RT   "Genomic minimalism in the early diverging intestinal parasite Giardia
RT   lamblia.";
RL   Science 317:1921-1926(2007).
CC   -!- FUNCTION: Plays an essential role in growth and, with Dicer, also
CC       involved in microRNA (miRNA)-mediated translational repression. The RNA
CC       interference pathway is implicated in antigenic variation having a role
CC       in regulation of variant-specific surface protein (VSP)-coding gene
CC       expression. Several VSP genes are transcribed but only transcripts
CC       encoding the VSP to be expressed accumulate. Antisense RNAs
CC       corresponding to the silenced VSP genes are detected (By similarity).
CC       {ECO:0000250|UniProtKB:Q86QW7}.
CC   -!- SUBUNIT: Interacts with miR2. Highly specific binding to the mRNA m7G-
CC       cap. May be a component of the RNA-induced silencing complex (RISC), a
CC       sequence-specific, multicomponent nuclease that destroys or silences
CC       messenger RNAs homologous to the silencing trigger (By similarity).
CC       {ECO:0000250|UniProtKB:Q86QW7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86QW7}.
CC   -!- DOMAIN: PAZ domain is absent, but Ago is capable of binding small RNAs
CC       in the same way as the other Argonaute proteins.
CC       {ECO:0000250|UniProtKB:Q86QW7}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AACB02000011; EDO80252.1; -; Genomic_DNA.
DR   RefSeq; XP_001707926.1; XM_001707874.1.
DR   AlphaFoldDB; A8BCK6; -.
DR   SMR; A8BCK6; -.
DR   STRING; 5741.EDO80252; -.
DR   EnsemblProtists; EDO80252; EDO80252; GL50803_2902.
DR   GeneID; 5700835; -.
DR   KEGG; gla:GL50803_002902; -.
DR   VEuPathDB; GiardiaDB:GL50803_2902; -.
DR   HOGENOM; CLU_008813_0_1_1; -.
DR   InParanoid; A8BCK6; -.
DR   OMA; LDRWAVI; -.
DR   OrthoDB; 220258at2759; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Developmental protein; Differentiation; RNA-binding;
KW   RNA-mediated gene silencing; Translation regulation.
FT   CHAIN           1..900
FT                   /note="Protein argonaute"
FT                   /id="PRO_0000405235"
FT   DOMAIN          555..879
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
SQ   SEQUENCE   900 AA;  100817 MW;  4B9D91A0E8136F45 CRC64;
     MVADVVTSRI NFYPYTVLPS ARPVYQYDLS IHISNQGYSL DNADSCRALE RFCEQQDAAL
     GNNLEHGLFY SLIIDFPSSD ACPISSFYSQ IDLGSRPITH TVEFLSTQKP RRRGGRVGGM
     RNNIAGPPIT PVQATVKVTR VRQMDPTDLM SMKILLNILL RRTFESSLGM LNIRSGFYDL
     RNPSIHAIQI DGRGYDICWI PGFRLTTATL RGKLGLQILP ETTKVRSKSM NELLTERRGN
     IHPSALAVIT VMAMHNGKVF RVHSIVQGQT IVSPLANGNE TDYFTYYSTR YRDKIDSAGL
     SLLRHNDYCN SVMQQDKFIL KLTPLKRTQN GKVVRPCNVP SSLCIIISDN EIAPYGVSKL
     STNRAAVALS TMSPDALLEK ATAFAARLAE DTELQSLLGD YGFGFTSNPL ELETFVCKPP
     KLMMDNTSRI VTIEDDSGGV FHNLLQSPGV SPIYYSNNNQ PATGMPVWAI MVPRNLGNDY
     ARRLRKELTE RVRSLAGTTA PNIGDPPLIA VELSNQRREM YRVEPYKDAF KALLNKLRLE
     YKDARESEIV AKIQLVVIVI PGPKQDRGDL YKGIKQFYTH MGIVTQCLLA PKSSQNELQW
     YDQAVLRSVC QQIYAKAGGA VWAPVLPPQN VYSKSTMLCA LDISRPKKTV GRPAEVPIST
     AGFISTYDGS FEYIYSQKKT LVPNRLNHGG ELQQQTLMEA FIKNSCNVYL AFNNRILPDH
     IVIFRDGVSD SQISATLEVE IKSLYECLRQ IYHKSNRPMC DLKVIVAQKT CAMRFSAVGG
     TVLRSGYYVI NRSPDNRQQG SEFLMASQAI VHGTTPKPIR YKIIFDSMEA SVDNDSFNQL
     IELTNAMAYG YVNWPQAISL PHVLHMAHHL SKFCGEVLRN GDDLFESCAI FGLQYRPFFI