ID   AGO_GIAIN               Reviewed;         781 AA.
AC   Q86QW7;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 2.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Protein argonaute {ECO:0000303|PubMed:19043559, ECO:0000303|PubMed:19079052};
DE            Short=GlAgo {ECO:0000303|PubMed:19043559};
GN   Name=Ago {ECO:0000303|PubMed:19043559};
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAO17548.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 30957 / WB {ECO:0000312|EMBL:AAO17548.1},
RC   WB1267 {ECO:0000269|PubMed:19079052}, and
RC   WB9B10 {ECO:0000269|PubMed:19079052};
RX   PubMed=19079052; DOI=10.1038/nature07585;
RA   Prucca C.G., Slavin I., Quiroga R., Elyas E.V., Rivero F.D., Saura A.,
RA   Carranza P.G., Lujan H.D.;
RT   "Antigenic variation in Giardia lamblia is regulated by RNA interference.";
RL   Nature 456:750-754(2008).
RN   [2] {ECO:0000305}
RP   FUNCTION, RNA-BINDING, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 30957 / WB {ECO:0000269|PubMed:19043559};
RC   TISSUE=Trophozoite {ECO:0000269|PubMed:19043559};
RX   PubMed=19043559; DOI=10.1371/journal.ppat.1000224;
RA   Saraiya A.A., Wang C.C.;
RT   "snoRNA, a novel precursor of microRNA in Giardia lamblia.";
RL   PLoS Pathog. 4:E1000224-E1000224(2008).
CC   -!- FUNCTION: Plays an essential role in growth and, with Dicer, also
CC       involved in microRNA (miRNA)-mediated translational repression. The RNA
CC       interference pathway is implicated in antigenic variation having a role
CC       in regulation of variant-specific surface protein (VSP)-coding gene
CC       expression. Several VSP genes are transcribed but only transcripts
CC       encoding the VSP to be expressed accumulate. Antisense RNAs
CC       corresponding to the silenced VSP genes are detected.
CC       {ECO:0000269|PubMed:19043559, ECO:0000269|PubMed:19079052}.
CC   -!- SUBUNIT: Interacts with miR2. Highly specific binding to the mRNA m7G-
CC       cap. May be a component of the RNA-induced silencing complex (RISC), a
CC       sequence-specific, multicomponent nuclease that destroys or silences
CC       messenger RNAs homologous to the silencing trigger.
CC       {ECO:0000269|PubMed:19043559, ECO:0000269|PubMed:19079052}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19079052}.
CC   -!- DOMAIN: PAZ domain is absent, but Ago is capable of binding small RNAs
CC       in the same way as the other Argonaute proteins.
CC       {ECO:0000269|PubMed:19043559}.
CC   -!- DISRUPTION PHENOTYPE: Inhibited cell growth due to inhibition of
CC       translation initiation. A change from single to multiple VSP expression
CC       in individual parasites. {ECO:0000269|PubMed:19043559,
CC       ECO:0000269|PubMed:19079052}.
CC   -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC       {ECO:0000255}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAO17548.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY142143; AAO17548.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q86QW7; -.
DR   SMR; Q86QW7; -.
DR   VEuPathDB; GiardiaDB:DHA2_152857; -.
DR   VEuPathDB; GiardiaDB:GL50581_2062; -.
DR   VEuPathDB; GiardiaDB:GL50803_002902; -.
DR   eggNOG; KOG1042; Eukaryota.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR003165; Piwi.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF02171; Piwi; 1.
DR   SMART; SM00950; Piwi; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS50822; PIWI; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Developmental protein; Differentiation; RNA-binding;
KW   RNA-mediated gene silencing; Translation regulation.
FT   CHAIN           1..781
FT                   /note="Protein argonaute"
FT                   /id="PRO_0000405236"
FT   DOMAIN          436..760
FT                   /note="Piwi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
SQ   SEQUENCE   781 AA;  87540 MW;  8372D20093DF8D6E CRC64;
     MRNNIAGPPI TPVQATVKVT RVRQMDPTDL MSMKILLNIL LRRTFESSLG MLNIRSGFYD
     LRNPSIHAIQ IDGRGYDICW IPGFRLTTAT LRGKLGLQIL PETTKVRSKS MNELLTERRG
     NIHPSALAVI TVMAMHNGKV FRVHSIVQGQ TIVSPLANGN ETDYFTYYST RYRDKIDSAG
     LSLLRHNDYC NSVMQQDKFI LKLTPLKRTQ NGKVVRPCNV PSSLCIIISD NEIAPYGVSK
     LSTNRAAVAL STMSPDALLE KATAFAARLA EDTELQSLLG DYGFGFTSNP LELETFVCKP
     PKLMMDNTSR IVTIEDDSGG VFHNLLQSPG VSPIYYSNNN QPATGMPVWA IMVPRNLGND
     YARRLRKELT ERVRSLAGTT APNIGDPPLI AVELSNQRRE MYRVEPYKDA FKALLNKLRL
     EYKDARESEI VAKIQLVVIV IPGPKQDRGD LYKGIKQFYT HMGIVTQCLL APKSSQNELQ
     WYDQAVLRSV CQQIYAKAGG AVWAPVLPPQ NVYSKSTMLC ALDISRPKKT VGRPAEVPIS
     TAGFISTYDG SFEYIYSQKK TLVPNRLNHG GELQQQTLME AFIKNSCNVY LAFNNRILPD
     HIVIFRDGVS DSQISATLEV EIKSLYECLR QIYHKSNRPM CDLKVIVAQK TCAMRFSAVG
     GTVLRSGYYV INRSPDNRQQ GSEFLMASQA IVHGTTPKPI RYKIIFDSME ASVDNDSFNQ
     LIELTNAMAY GYVNWPQAIS LPHVLHMAHH LSKFCGEVLR NGDDLFESCA IFGLQYRPFF
     I