ERA_HELPY
ID ERA_HELPY Reviewed; 301 AA.
AC P56059; P96520;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=GTPase Era {ECO:0000255|HAMAP-Rule:MF_00367};
GN Name=era {ECO:0000255|HAMAP-Rule:MF_00367}; OrderedLocusNames=HP_0517;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Akopyants N.S., Kersulyte D., Clifton S.W., Youree B.E., Reece C.A.,
RA Roe B.A., Berg D.E.;
RT "The Cag pathogenicity island of Helicobacter pylori.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: An essential GTPase that binds both GDP and GTP, with rapid
CC nucleotide exchange. Plays a role in 16S rRNA processing and 30S
CC ribosomal subunit biogenesis and possibly also in cell cycle regulation
CC and energy metabolism. {ECO:0000255|HAMAP-Rule:MF_00367}.
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00367}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell inner membrane
CC {ECO:0000255|HAMAP-Rule:MF_00367}; Peripheral membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00367}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. Era GTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_00367, ECO:0000255|PROSITE-ProRule:PRU01050}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD07585.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE000511; AAD07585.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC000108; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; E64584; E64584.
DR RefSeq; NP_207314.1; NC_000915.1.
DR RefSeq; WP_001862438.1; NC_000915.1.
DR AlphaFoldDB; P56059; -.
DR SMR; P56059; -.
DR STRING; 85962.C694_02660; -.
DR PaxDb; P56059; -.
DR EnsemblBacteria; AAD07585; AAD07585; HP_0517.
DR KEGG; hpy:HP_0517; -.
DR PATRIC; fig|85962.47.peg.556; -.
DR eggNOG; COG1159; Bacteria.
DR OMA; WAEVDVI; -.
DR PhylomeDB; P56059; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0070181; F:small ribosomal subunit rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR CDD; cd04163; Era; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00367; GTPase_Era; 1.
DR InterPro; IPR030388; G_ERA_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR005662; GTP-bd_Era.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR004044; KH_dom_type_2.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR PANTHER; PTHR42698; PTHR42698; 1.
DR Pfam; PF07650; KH_2; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54814; SSF54814; 1.
DR TIGRFAMs; TIGR00436; era; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51713; G_ERA; 1.
DR PROSITE; PS50823; KH_TYPE_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cytoplasm; GTP-binding; Membrane;
KW Nucleotide-binding; Reference proteome; Ribosome biogenesis; RNA-binding;
KW rRNA-binding.
FT CHAIN 1..301
FT /note="GTPase Era"
FT /id="PRO_0000180018"
FT DOMAIN 4..173
FT /note="Era-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT DOMAIN 204..280
FT /note="KH type-2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367"
FT REGION 12..19
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 38..42
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 64..67
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 122..125
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 152..154
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367"
FT BINDING 64..68
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367"
FT BINDING 122..125
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00367"
FT CONFLICT 55
FT /note="W -> G (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="S -> N (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="Q -> K (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="D -> S (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="A -> D (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="A -> T (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 169..170
FT /note="QH -> KY (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="N -> S (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
FT CONFLICT 296..297
FT /note="HR -> YQ (in Ref. 2; AC000108)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 34591 MW; BB9283013A3AA07C CRC64;
MKTKAGFVAL IGKPNAGKST LLNTLLNAHL ALVSHKANAT RKLMKCIVPF KDKEWYESQI
IFLDTPGLHH QEKLLNQCML SQALKAMGDA ELCVFLASVH DDLKGYEEFL SLCQKPHILA
LSKIDTATHK QVLQKLQEYQ QYDSQFLALV PLSAKKSQNL NALLECISQH LSPSAWLFEK
DLMSDEKMRD IYKEIIRESL FDFLSDEIPY ESDVMIDKFI EEERIDKVYA RIIVEKESQK
KIVIGKNGVN IKRIGTNARL KMQEVGEKKV FLNLQVIAQK SWSKEEKSLQ KLGYIHRRNR
D
