AGP12_ARATH
ID AGP12_ARATH Reviewed; 60 AA.
AC Q9LJD9;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Arabinogalactan protein 12 {ECO:0000303|PubMed:11006345};
DE Short=AtAGP12 {ECO:0000303|PubMed:11006345};
DE AltName: Full=Arabinogalactan peptide 12 {ECO:0000303|PubMed:11006345};
DE Short=AG-peptide 12 {ECO:0000303|PubMed:11006345};
DE Flags: Precursor;
GN Name=AGP12 {ECO:0000303|PubMed:11006345}; OrderedLocusNames=At3g13520;
GN ORFNames=MRP15.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-38, HYDROXYLATION AT
RP PRO-32; PRO-34 AND PRO-36, AND PYROGLUTAMATE FORMATION AT GLN-28.
RC STRAIN=cv. Columbia;
RX PubMed=11006345; DOI=10.2307/3871187;
RA Schultz C.J., Johnson K.L., Currie G., Bacic A.;
RT "The classical arabinogalactan protein gene family of Arabidopsis.";
RL Plant Cell 12:1751-1767(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PROTEIN SEQUENCE OF 28-38, HYDROXYLATION AT PRO-32; PRO-34 AND PRO-36,
RP PYROGLUTAMATE FORMATION AT GLN-28, GLYCOSYLATION AT PRO-32; PRO-34 AND
RP PRO-36, AND GPI-ANCHOR AT SER-38.
RX PubMed=15322080; DOI=10.1074/jbc.m407594200;
RA Schultz C.J., Ferguson K.L., Lahnstein J., Bacic A.;
RT "Post-translational modifications of arabinogalactan-peptides of
RT Arabidopsis thaliana. Endoplasmic reticulum and
RT glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation
RT of proline.";
RL J. Biol. Chem. 279:45503-45511(2004).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12177459; DOI=10.1104/pp.003459;
RA Schultz C.J., Rumsewicz M.P., Johnson K.L., Jones B.J., Gaspar Y.M.,
RA Bacic A.;
RT "Using genomic resources to guide research directions. The arabinogalactan
RT protein gene family as a test case.";
RL Plant Physiol. 129:1448-1463(2002).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=25053647; DOI=10.1093/jxb/eru300;
RA Pereira A.M., Masiero S., Nobre M.S., Costa M.L., Solis M.T.,
RA Testillano P.S., Sprunck S., Coimbra S.;
RT "Differential expression patterns of arabinogalactan proteins in
RT Arabidopsis thaliana reproductive tissues.";
RL J. Exp. Bot. 65:5459-5471(2014).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000269|PubMed:15322080}.
CC -!- TISSUE SPECIFICITY: Expressed in reproductive tissues. Expressed in
CC chalaza, funiculus, stigma, septum, style and transmitting tract.
CC {ECO:0000269|PubMed:25053647}.
CC -!- PTM: Contains 4-hydroxyproline; hydroxylated on Pro-32, Pro-34 and Pro-
CC 36. {ECO:0000269|PubMed:11006345, ECO:0000269|PubMed:15322080}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC {ECO:0000305|PubMed:15322080}.
CC -!- SIMILARITY: Belongs to the AG-peptide AGP family. {ECO:0000305}.
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DR EMBL; AF195893; AAG24280.1; -; mRNA.
DR EMBL; AP000603; BAB01759.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75367.1; -; Genomic_DNA.
DR EMBL; AY059924; AAL24406.1; -; mRNA.
DR EMBL; AY081598; AAM10160.1; -; mRNA.
DR EMBL; AY086039; AAM63249.1; -; mRNA.
DR RefSeq; NP_566458.1; NM_112198.3.
DR AlphaFoldDB; Q9LJD9; -.
DR BioGRID; 5888; 5.
DR IntAct; Q9LJD9; 5.
DR STRING; 3702.AT3G13520.1; -.
DR PaxDb; Q9LJD9; -.
DR PRIDE; Q9LJD9; -.
DR EnsemblPlants; AT3G13520.1; AT3G13520.1; AT3G13520.
DR GeneID; 820554; -.
DR Gramene; AT3G13520.1; AT3G13520.1; AT3G13520.
DR KEGG; ath:AT3G13520; -.
DR Araport; AT3G13520; -.
DR TAIR; locus:2092880; AT3G13520.
DR HOGENOM; CLU_183441_3_0_1; -.
DR InParanoid; Q9LJD9; -.
DR OMA; PPPITAC; -.
DR OrthoDB; 1639774at2759; -.
DR PhylomeDB; Q9LJD9; -.
DR PRO; PR:Q9LJD9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJD9; baseline and differential.
DR Genevisible; Q9LJD9; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR039281; AGP3/12/13/14/21.
DR PANTHER; PTHR34114; PTHR34114; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan;
KW Pyrrolidone carboxylic acid; Reference proteome; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT PEPTIDE 28..38
FT /note="Arabinogalactan protein 12"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT /id="PRO_0000269011"
FT PROPEP 39..60
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:11006345,
FT ECO:0000305|PubMed:15322080"
FT /id="PRO_0000269012"
FT MOD_RES 28
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT MOD_RES 32
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT MOD_RES 34
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT MOD_RES 36
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT LIPID 38
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000269|PubMed:15322080"
FT CARBOHYD 32
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 34
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 36
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
SQ SEQUENCE 60 AA; 6089 MW; ECBAE55B6471D687 CRC64;
MESMKMKLIV VLMVAIVAFS AVGNVAAQTE APAPSPTSDA AMFVPALFAS VAALASGFLF
