AGP14_ARATH
ID AGP14_ARATH Reviewed; 60 AA.
AC Q9LVC0;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Arabinogalactan protein 14 {ECO:0000303|PubMed:11006345};
DE Short=AtAGP14 {ECO:0000303|PubMed:11006345};
DE AltName: Full=Arabinogalactan peptide 14 {ECO:0000303|PubMed:11006345};
DE Short=AG-peptide 14 {ECO:0000303|PubMed:11006345};
DE Flags: Precursor;
GN Name=AGP14 {ECO:0000303|PubMed:11006345}; OrderedLocusNames=At5g56540;
GN ORFNames=MKN22.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], HYDROXYLATION AT PRO-32; PRO-34 AND PRO-36, AND
RP PROTEIN SEQUENCE OF 29-38.
RC STRAIN=cv. Columbia;
RX PubMed=11006345; DOI=10.2307/3871187;
RA Schultz C.J., Johnson K.L., Currie G., Bacic A.;
RT "The classical arabinogalactan protein gene family of Arabidopsis.";
RL Plant Cell 12:1751-1767(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP PROTEIN SEQUENCE OF 29-38, HYDROXYLATION AT PRO-32; PRO-34 AND PRO-36,
RP GLYCOSYLATION AT PRO-32; PRO-34 AND PRO-36, AND GPI-ANCHOR AT SER-38.
RX PubMed=15322080; DOI=10.1074/jbc.m407594200;
RA Schultz C.J., Ferguson K.L., Lahnstein J., Bacic A.;
RT "Post-translational modifications of arabinogalactan-peptides of
RT Arabidopsis thaliana. Endoplasmic reticulum and
RT glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation
RT of proline.";
RL J. Biol. Chem. 279:45503-45511(2004).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12177459; DOI=10.1104/pp.003459;
RA Schultz C.J., Rumsewicz M.P., Johnson K.L., Jones B.J., Gaspar Y.M.,
RA Bacic A.;
RT "Using genomic resources to guide research directions. The arabinogalactan
RT protein gene family as a test case.";
RL Plant Physiol. 129:1448-1463(2002).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21248074; DOI=10.1104/pp.110.166520;
RA Lin W.D., Liao Y.Y., Yang T.J., Pan C.Y., Buckhout T.J., Schmidt W.;
RT "Coexpression-based clustering of Arabidopsis root genes predicts
RT functional modules in early phosphate deficiency signaling.";
RL Plant Physiol. 155:1383-1402(2011).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death (Probable). Involved in the
CC regulation of root hair elongation (PubMed:21248074).
CC {ECO:0000269|PubMed:21248074, ECO:0000305}.
CC -!- INTERACTION:
CC Q9LVC0; Q94F58: NAC089; NbExp=2; IntAct=EBI-4429269, EBI-2319707;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000269|PubMed:15322080}.
CC -!- PTM: Contains 4-hydroxyproline; hydroxylated on Pro-32, Pro-34 and Pro-
CC 36. {ECO:0000269|PubMed:11006345, ECO:0000269|PubMed:15322080}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC {ECO:0000305|PubMed:15322080}.
CC -!- DISRUPTION PHENOTYPE: Formation of abnormal long root hairs.
CC {ECO:0000269|PubMed:21248074}.
CC -!- SIMILARITY: Belongs to the AG-peptide AGP family. {ECO:0000305}.
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DR EMBL; AF195895; AAG24282.1; -; mRNA.
DR EMBL; AB019234; BAA97180.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96779.1; -; Genomic_DNA.
DR EMBL; AK117330; BAC42000.1; -; mRNA.
DR EMBL; BT004644; AAO42890.1; -; mRNA.
DR RefSeq; NP_200465.1; NM_125037.3.
DR AlphaFoldDB; Q9LVC0; -.
DR BioGRID; 20999; 3.
DR IntAct; Q9LVC0; 3.
DR STRING; 3702.AT5G56540.1; -.
DR PaxDb; Q9LVC0; -.
DR EnsemblPlants; AT5G56540.1; AT5G56540.1; AT5G56540.
DR GeneID; 835755; -.
DR Gramene; AT5G56540.1; AT5G56540.1; AT5G56540.
DR KEGG; ath:AT5G56540; -.
DR Araport; AT5G56540; -.
DR TAIR; locus:2167126; AT5G56540.
DR eggNOG; ENOG502S9IR; Eukaryota.
DR HOGENOM; CLU_183441_3_0_1; -.
DR InParanoid; Q9LVC0; -.
DR OMA; MMMTIMA; -.
DR PhylomeDB; Q9LVC0; -.
DR PRO; PR:Q9LVC0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVC0; baseline and differential.
DR Genevisible; Q9LVC0; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR InterPro; IPR039281; AGP3/12/13/14/21.
DR PANTHER; PTHR34114; PTHR34114; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan; Reference proteome;
KW Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT PEPTIDE 29..38
FT /note="Arabinogalactan protein 14"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT /id="PRO_0000269015"
FT PROPEP 39..60
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:11006345,
FT ECO:0000305|PubMed:15322080"
FT /id="PRO_0000269016"
FT MOD_RES 32
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT MOD_RES 34
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT MOD_RES 36
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345,
FT ECO:0000269|PubMed:15322080"
FT LIPID 38
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000269|PubMed:15322080"
FT CARBOHYD 32
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 34
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 36
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
SQ SEQUENCE 60 AA; 6363 MW; 83F3A3185C53C0B4 CRC64;
MEAMKMKLYV VVLVAVIAFS TVHQTVAAVD APAPSPTSDA SSFIPTFFAS VAVMAFGFFF
