ERA_ORYSJ
ID ERA_ORYSJ Reviewed; 423 AA.
AC B9FI63; C7J2L9; Q688W4;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=GTPase ERA-like, chloroplastic {ECO:0000305};
DE AltName: Full=GTP-binding protein Era-like {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=Os05g0567300 {ECO:0000312|EMBL:BAS95385.1},
GN LOC_Os05g49220 {ECO:0000305};
GN ORFNames=OJ1781_H11.13 {ECO:0000312|EMBL:AAU10665.1},
GN OsJ_19577 {ECO:0000312|EMBL:EEE64721.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Nuclear genome-encoded probable GTPase involved in ribosome
CC biogenesis in chloroplasts. Plays a role in 16S rRNA maturation in
CC plastids and may contribute to the assembly of the small (30S)
CC ribosomal subunit. {ECO:0000250|UniProtKB:K7UTH7}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid
CC {ECO:0000250|UniProtKB:K7UTH7}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. Era GTPase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01050, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAU10665.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAG99534.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=BAH93259.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAS95385.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC120986; AAU10665.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAH93259.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS95385.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000142; EEE64721.1; -; Genomic_DNA.
DR EMBL; AK112068; BAG99534.1; ALT_SEQ; mRNA.
DR AlphaFoldDB; B9FI63; -.
DR SMR; B9FI63; -.
DR STRING; 4530.OS05T0567300-01; -.
DR PRIDE; B9FI63; -.
DR eggNOG; KOG1423; Eukaryota.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0042644; C:chloroplast nucleoid; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR GO; GO:0042254; P:ribosome biogenesis; ISS:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR CDD; cd04163; Era; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00367; GTPase_Era; 1.
DR InterPro; IPR030388; G_ERA_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR005662; GTP-bd_Era.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR004044; KH_dom_type_2.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR PANTHER; PTHR42698; PTHR42698; 1.
DR Pfam; PF07650; KH_2; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54814; SSF54814; 1.
DR TIGRFAMs; TIGR00436; era; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51713; G_ERA; 1.
DR PROSITE; PS50823; KH_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; GTP-binding; Nucleotide-binding; Plastid; Reference proteome;
KW Ribosome biogenesis; RNA-binding; Transit peptide.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 61..423
FT /note="GTPase ERA-like, chloroplastic"
FT /id="PRO_0000441336"
FT DOMAIN 124..294
FT /note="Era-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT DOMAIN 325..402
FT /note="KH type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00118"
FT REGION 132..139
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 158..162
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 179..182
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 244..247
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT REGION 273..275
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01050"
FT BINDING 132..139
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 179..183
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
FT BINDING 244..247
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P06616"
SQ SEQUENCE 423 AA; 47862 MW; FB469205B7022EF4 CRC64;
MELGLALRLV APPPRLPCRA LQPPPMPCFS PCAARRSRIR SSRLERRVGV VVSGGSMASL
AMEEEEEEEW EEAEEEAEGW QEEEAAVVTT RPRLELIEKP DRSLCLLDEY ESEELGTSHC
ANHRSGYVAV LGKPNVGKST LINQIVGQKL SIVTDKPQTT RHRILGICSE PEYQIILYDT
PGVIKKEMHK LDTMMMKNVR SAVGSADCVL VVVDACKMPE KIDEILEEGV GNKDTELPVL
LVLNKKDLIK PGEIAKKLEW YQKFTNADDV IPISAKFGHG VDDIKEWILS KLPLGPAYYP
KDIASEHPER FFVGEIVREK IFLQYRQEIP YACQVNVISY KSRPTAKDFI QVEILVEKES
QRSIILGKDG KAIKMLATAS RLDIEDFLQK KVYLEIMVKV KENWRQDELL LKRYGYGGEI
QAL
