AGP20_ARATH
ID AGP20_ARATH Reviewed; 74 AA.
AC Q9M373;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Arabinogalactan protein 20 {ECO:0000303|PubMed:12177459};
DE Short=AtAGP20 {ECO:0000303|PubMed:12177459};
DE AltName: Full=Arabinogalactan peptide 20 {ECO:0000303|PubMed:12177459};
DE Short=AG-peptide 20 {ECO:0000303|PubMed:12177459};
DE Flags: Precursor;
GN Name=AGP20 {ECO:0000303|PubMed:12177459}; OrderedLocusNames=At3g61640;
GN ORFNames=F15G16.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 27-37, HYDROXYLATION AT PRO-31; PRO-33 AND PRO-35,
RP PYROGLUTAMATE FORMATION AT GLN-27, GLYCOSYLATION AT PRO-31; PRO-33 AND
RP PRO-35, AND GPI-ANCHOR AT SER-37.
RX PubMed=15322080; DOI=10.1074/jbc.m407594200;
RA Schultz C.J., Ferguson K.L., Lahnstein J., Bacic A.;
RT "Post-translational modifications of arabinogalactan-peptides of
RT Arabidopsis thaliana. Endoplasmic reticulum and
RT glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation
RT of proline.";
RL J. Biol. Chem. 279:45503-45511(2004).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12177459; DOI=10.1104/pp.003459;
RA Schultz C.J., Rumsewicz M.P., Johnson K.L., Jones B.J., Gaspar Y.M.,
RA Bacic A.;
RT "Using genomic resources to guide research directions. The arabinogalactan
RT protein gene family as a test case.";
RL Plant Physiol. 129:1448-1463(2002).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- PTM: Contains 4-hydroxyproline; hydroxylated on Pro-31, Pro-33 and Pro-
CC 35. {ECO:0000269|PubMed:15322080}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC {ECO:0000305|PubMed:15322080}.
CC -!- SIMILARITY: Belongs to the AG-peptide AGP family. {ECO:0000305}.
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DR EMBL; AL132959; CAB71094.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80235.1; -; Genomic_DNA.
DR EMBL; AY084640; AAM61203.1; -; mRNA.
DR EMBL; BT025563; ABF58981.1; -; mRNA.
DR PIR; T47956; T47956.
DR RefSeq; NP_191723.1; NM_116029.3.
DR AlphaFoldDB; Q9M373; -.
DR STRING; 3702.AT3G61640.1; -.
DR PaxDb; Q9M373; -.
DR EnsemblPlants; AT3G61640.1; AT3G61640.1; AT3G61640.
DR GeneID; 825337; -.
DR Gramene; AT3G61640.1; AT3G61640.1; AT3G61640.
DR KEGG; ath:AT3G61640; -.
DR Araport; AT3G61640; -.
DR TAIR; locus:2076730; AT3G61640.
DR eggNOG; ENOG502S708; Eukaryota.
DR HOGENOM; CLU_187330_0_0_1; -.
DR InParanoid; Q9M373; -.
DR OMA; AYLLMIM; -.
DR OrthoDB; 1641401at2759; -.
DR PhylomeDB; Q9M373; -.
DR PRO; PR:Q9M373; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M373; baseline and differential.
DR Genevisible; Q9M373; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR009424; AGP16/20/22/41.
DR PANTHER; PTHR33374; PTHR33374; 1.
DR Pfam; PF06376; AGP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan;
KW Pyrrolidone carboxylic acid; Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000269|PubMed:15322080"
FT PEPTIDE 27..37
FT /note="Arabinogalactan protein 20"
FT /evidence="ECO:0000269|PubMed:15322080"
FT /id="PRO_0000269021"
FT PROPEP 38..74
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:15322080"
FT /id="PRO_0000269022"
FT MOD_RES 27
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 31
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 33
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 35
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT LIPID 37
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000269|PubMed:15322080"
FT CARBOHYD 31
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 33
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 35
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
SQ SEQUENCE 74 AA; 7699 MW; F47D5BC7911C2C0A CRC64;
MASRNSVAVI ALFAFVFAVI SPFAGAQSLA PAPSPTSDGT SIDQGIAYLL MVVALVLTYL
IHPLDASSSS YTFF
