AGP22_ARATH
ID AGP22_ARATH Reviewed; 63 AA.
AC Q9FK16;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Arabinogalactan protein 22 {ECO:0000303|PubMed:12177459};
DE Short=AtAGP22 {ECO:0000303|PubMed:12177459};
DE AltName: Full=Arabinogalactan peptide 22 {ECO:0000303|PubMed:12177459};
DE Short=AG-peptide 22 {ECO:0000303|PubMed:12177459};
DE Flags: Precursor;
GN Name=AGP22 {ECO:0000303|PubMed:12177459}; OrderedLocusNames=At5g53250;
GN ORFNames=K19E1.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 28-38, HYDROXYLATION AT PRO-32; PRO-34 AND PRO-36,
RP GLYCOSYLATION AT PRO-32; PRO-34 AND PRO-36, AND GPI-ANCHOR AT SER-38.
RX PubMed=15322080; DOI=10.1074/jbc.m407594200;
RA Schultz C.J., Ferguson K.L., Lahnstein J., Bacic A.;
RT "Post-translational modifications of arabinogalactan-peptides of
RT Arabidopsis thaliana. Endoplasmic reticulum and
RT glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation
RT of proline.";
RL J. Biol. Chem. 279:45503-45511(2004).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12177459; DOI=10.1104/pp.003459;
RA Schultz C.J., Rumsewicz M.P., Johnson K.L., Jones B.J., Gaspar Y.M.,
RA Bacic A.;
RT "Using genomic resources to guide research directions. The arabinogalactan
RT protein gene family as a test case.";
RL Plant Physiol. 129:1448-1463(2002).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000269|PubMed:15322080}.
CC -!- PTM: Contains 4-hydroxyproline; hydroxylated on Pro-32, Pro-34 and Pro-
CC 36. {ECO:0000269|PubMed:15322080}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC {ECO:0000305|PubMed:15322080}.
CC -!- SIMILARITY: Belongs to the AG-peptide AGP family. {ECO:0000305}.
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DR EMBL; AB013388; BAB09787.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96329.1; -; Genomic_DNA.
DR EMBL; BT010777; AAR24144.1; -; mRNA.
DR EMBL; BT011258; AAR92294.1; -; mRNA.
DR EMBL; AK228915; BAF00804.1; -; mRNA.
DR RefSeq; NP_200137.1; NM_124704.3.
DR AlphaFoldDB; Q9FK16; -.
DR STRING; 3702.AT5G53250.1; -.
DR PaxDb; Q9FK16; -.
DR EnsemblPlants; AT5G53250.1; AT5G53250.1; AT5G53250.
DR GeneID; 835406; -.
DR Gramene; AT5G53250.1; AT5G53250.1; AT5G53250.
DR KEGG; ath:AT5G53250; -.
DR Araport; AT5G53250; -.
DR TAIR; locus:2154232; AT5G53250.
DR HOGENOM; CLU_187330_0_0_1; -.
DR InParanoid; Q9FK16; -.
DR OMA; MSVACSQ; -.
DR PhylomeDB; Q9FK16; -.
DR PRO; PR:Q9FK16; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FK16; baseline and differential.
DR Genevisible; Q9FK16; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR009424; AGP16/20/22/41.
DR PANTHER; PTHR33374; PTHR33374; 1.
DR Pfam; PF06376; AGP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan; Reference proteome;
KW Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000269|PubMed:15322080"
FT PEPTIDE 28..38
FT /note="Arabinogalactan protein 22"
FT /evidence="ECO:0000269|PubMed:15322080"
FT /id="PRO_0000269025"
FT PROPEP 39..63
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:15322080"
FT /id="PRO_0000269026"
FT MOD_RES 32
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 34
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 36
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT LIPID 38
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000269|PubMed:15322080"
FT CARBOHYD 32
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 34
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 36
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
SQ SEQUENCE 63 AA; 6672 MW; 04606C7635B3E08C CRC64;
MASLKFPLEI LAVFVIISVI LLPIAQSHSS SPAPAPTSDG TSIDQGIAYV LMMVALALTY
FIH
