ERCC3_DANRE
ID ERCC3_DANRE Reviewed; 782 AA.
AC Q7ZVV1;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=General transcription and DNA repair factor IIH helicase subunit XPB;
DE Short=TFIIH subunit XPB;
DE EC=3.6.4.12;
DE AltName: Full=DNA excision repair protein ERCC-3;
GN Name=ercc3;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ATP-dependent 3'-5' DNA helicase, component of the general
CC transcription and DNA repair factor IIH (TFIIH) core complex, which is
CC involved in general and transcription-coupled nucleotide excision
CC repair (NER) of damaged DNA and, when complexed to CAK, in RNA
CC transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA
CC around the lesion to allow the excision of the damaged oligonucleotide
CC and its replacement by a new DNA fragment. The ATPase activity of
CC XPB/ERCC3, but not its helicase activity, is required for DNA opening.
CC In transcription, TFIIH has an essential role in transcription
CC initiation. When the pre-initiation complex (PIC) has been established,
CC TFIIH is required for promoter opening and promoter escape. The ATP-
CC dependent helicase activity of XPB/ERCC3 is required for promoter
CC opening and promoter escape. Phosphorylation of the C-terminal tail
CC (CTD) of the largest subunit of RNA polymerase II by the kinase module
CC CAK controls the initiation of transcription.
CC {ECO:0000250|UniProtKB:P19447}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which
CC is active in NER. The core complex associates with the 3-subunit CDK-
CC activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and
CC MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. Interacts with PUF60. Interacts with ATF7IP. Interacts
CC with Epstein-Barr virus EBNA2. {ECO:0000250|UniProtKB:P19447}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC {ECO:0000305}.
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DR EMBL; BC045400; AAH45400.1; -; mRNA.
DR RefSeq; NP_963876.1; NM_201582.1.
DR AlphaFoldDB; Q7ZVV1; -.
DR SMR; Q7ZVV1; -.
DR STRING; 7955.ENSDARP00000105166; -.
DR PaxDb; Q7ZVV1; -.
DR PRIDE; Q7ZVV1; -.
DR GeneID; 324323; -.
DR KEGG; dre:324323; -.
DR CTD; 2071; -.
DR ZFIN; ZDB-GENE-030131-3043; ercc3.
DR eggNOG; KOG0159; Eukaryota.
DR eggNOG; KOG1123; Eukaryota.
DR InParanoid; Q7ZVV1; -.
DR OrthoDB; 100698at2759; -.
DR PhylomeDB; Q7ZVV1; -.
DR Reactome; R-DRE-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DRE-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-DRE-5696400; Dual Incision in GG-NER.
DR Reactome; R-DRE-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DRE-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DRE-6782135; Dual incision in TC-NER.
DR Reactome; R-DRE-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DRE-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DRE-72086; mRNA Capping.
DR Reactome; R-DRE-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-DRE-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-DRE-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DRE-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DRE-73863; RNA Polymerase I Transcription Termination.
DR Reactome; R-DRE-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DRE-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DRE-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DRE-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:Q7ZVV1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:UniProtKB.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; ISS:UniProtKB.
DR GO; GO:0006265; P:DNA topological change; ISS:UniProtKB.
DR GO; GO:0033683; P:nucleotide-excision repair, DNA incision; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006283; P:transcription-coupled nucleotide-excision repair; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR032438; ERCC3_RAD25_C.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001161; XPB/Ssl2.
DR InterPro; IPR032830; XPB/Ssl2_N.
DR Pfam; PF16203; ERCC3_RAD25_C; 1.
DR Pfam; PF13625; Helicase_C_3; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00603; rad25; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..782
FT /note="General transcription and DNA repair factor IIH
FT helicase subunit XPB"
FT /id="PRO_0000323744"
FT DOMAIN 326..487
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 541..701
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 6..17
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 440..443
FT /note="DEVH box"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 339..346
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 782 AA; 89288 MW; 5CAA94DA15CA7A15 CRC64;
MGRKDKSDRE KKSKKRYYED EEEDEEVIGG ESQEAVPAAA GKQVDESSTK LDEYGAKDYR
LQMLLKNDHS SRPLWVAPDG HIFLEAFSPV YKYAQDFLVA ISEPVCRPTH AHEYKLTAYS
LYAAVSVGLQ TSDIIEYLQK LSKTSVPDGI VQFIKLCTVS YGKVKLVLKH NRYFVESAFP
DVIQRLLQDT VIRDCRLRSA EGEETELITE TISSKSAISK SQQDNGGPSS SQPADGQRSG
TQVPEDIFSY YEQMDKEEEE EEETQTVSFE IRQEMIEELQ KRCIQLEYPL LAEYDFRNDT
VNPDINMDLK PTAVLRPYQE KSLRKMFGNG RARSGVIVLP CGAGKSLVGV TAACTVRKRC
LVLGNSSVSV EQWKAQFKMW STIDDSQICR FTSDAKDKPI GCSVAISTYS MLGHTTKRSW
EAERVMEWMK SQEWGLIILD EVHTIPAKMF RRVLTIVQAH CKLGLTATLV REDDKIVDLN
FLIGPKLYEA NWMELQNNGY IAKVQCAEVW CPMSPEFYRE YVAIKTKKRI LLYTMNPNKF
RACQFLIRFH ERRNDKIIVF ADNVFALKEY AIRLNKPYIY GPTSQGERMQ ILQNFKHNPK
INTIFISKVG DTSFDLPEAN VLIQISSHGG SRRQEAQRLG RVLRAKKGMV AEEYNAYFYS
LVSQDTQEMA YSTKRQRFLV DQGYSFKVIT KLAGMEEEDL MFSTRDEQQQ LLQKVLAASD
LDAEEEVVMG EVGGKPQFSR RAGTMSSMSG ADDALYMEYQ MPRGSKASVG KNIHPLFKRF
RK
