AGP40_ARATH
ID AGP40_ARATH Reviewed; 62 AA.
AC Q8LD43; A0MEX4;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Arabinogalactan protein 40 {ECO:0000303|PubMed:15322080};
DE Short=AtAGP40 {ECO:0000303|PubMed:15322080};
DE AltName: Full=Arabinogalactan peptide 40 {ECO:0000303|PubMed:15322080};
DE Short=AG-peptide 40 {ECO:0000303|PubMed:15322080};
DE Flags: Precursor;
GN Name=AGP40 {ECO:0000303|PubMed:15322080};
GN OrderedLocusNames=At3g20865 {ECO:0000312|Araport:AT3G20865};
GN ORFNames=MOE17 {ECO:0000312|EMBL:AB025629};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PROTEIN SEQUENCE OF 23-35, HYDROXYLATION AT PRO-28; PRO-30 AND PRO-32,
RP GLYCOSYLATION AT PRO-28; PRO-30 AND PRO-32, AND GPI-ANCHOR AT SER-35.
RX PubMed=15322080; DOI=10.1074/jbc.m407594200;
RA Schultz C.J., Ferguson K.L., Lahnstein J., Bacic A.;
RT "Post-translational modifications of arabinogalactan-peptides of
RT Arabidopsis thaliana. Endoplasmic reticulum and
RT glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation
RT of proline.";
RL J. Biol. Chem. 279:45503-45511(2004).
RN [7]
RP INDUCTION.
RX PubMed=25336567; DOI=10.1093/mp/ssu114;
RA Jia Q.S., Zhu J., Xu X.F., Lou Y., Zhang Z.L., Zhang Z.P., Yang Z.N.;
RT "Arabidopsis AT-hook protein TEK positively regulates the expression of
RT arabinogalactan proteins in controlling nexine layer formation in the
RT pollen wall.";
RL Mol. Plant 0:0-0(2014).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Lipid-anchor, GPI-
CC anchor {ECO:0000269|PubMed:15322080}.
CC -!- INDUCTION: Up-regulated by AHL16/TEK. {ECO:0000269|PubMed:25336567}.
CC -!- PTM: Contains 4-hydroxyproline; hydroxylated on Pro-28, Pro-30 and Pro-
CC 32. {ECO:0000269|PubMed:15322080}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC {ECO:0000305|PubMed:15322080}.
CC -!- SIMILARITY: Belongs to the AG-peptide AGP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28566.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB025629; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE76433.1; -; Genomic_DNA.
DR EMBL; DQ446680; ABE65953.1; -; mRNA.
DR EMBL; DQ653097; ABK28566.1; ALT_SEQ; mRNA.
DR EMBL; BT026389; ABH04496.1; -; mRNA.
DR EMBL; AY086220; AAM67312.1; -; mRNA.
DR RefSeq; NP_566668.1; NM_112978.1.
DR AlphaFoldDB; Q8LD43; -.
DR IntAct; Q8LD43; 1.
DR STRING; 3702.AT3G20865.1; -.
DR PaxDb; Q8LD43; -.
DR EnsemblPlants; AT3G20865.1; AT3G20865.1; AT3G20865.
DR GeneID; 821635; -.
DR Gramene; AT3G20865.1; AT3G20865.1; AT3G20865.
DR KEGG; ath:AT3G20865; -.
DR Araport; AT3G20865; -.
DR TAIR; locus:505006358; AT3G20865.
DR HOGENOM; CLU_2907166_0_0_1; -.
DR InParanoid; Q8LD43; -.
DR OMA; QMRNVNN; -.
DR PRO; PR:Q8LD43; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LD43; baseline and differential.
DR Genevisible; Q8LD43; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR044702; AGP23/40.
DR PANTHER; PTHR34672; PTHR34672; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan; Reference proteome;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:15322080"
FT PEPTIDE 23..35
FT /note="Arabinogalactan protein 40"
FT /evidence="ECO:0000269|PubMed:15322080"
FT /id="PRO_0000432047"
FT PROPEP 36..62
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:15322080"
FT /id="PRO_0000432048"
FT MOD_RES 28
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 30
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT MOD_RES 32
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:15322080"
FT LIPID 35
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000269|PubMed:15322080"
FT CARBOHYD 28
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 30
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
FT CARBOHYD 32
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000305|PubMed:15322080"
SQ SEQUENCE 62 AA; 6156 MW; B99225D6C07D9104 CRC64;
MEMKNIFVAL FISAVLVSSV SAATMESPAP SPGASSASTV AFPVVGSIVA ASLSAFLALL
LQ
