AGP4_ARATH
ID AGP4_ARATH Reviewed; 135 AA.
AC Q9ZT16;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Classical arabinogalactan protein 4;
DE Flags: Precursor;
GN Name=AGP4; OrderedLocusNames=At5g10430; ORFNames=F12B17_220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Schultz C.J., Gilson P.R., Oxley D., Youl J.J., Bacic A.;
RT "GPI-anchors on arabinogalactan-proteins: implications for signalling in
RT plants.";
RL Trends Plant Sci. 3:426-431(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10451111;
RX DOI=10.1002/(sici)1522-2683(19990701)20:10<2027::aid-elps2027>3.0.co;2-a;
RA Sherrier D.J., Prime T.A., Dupree P.;
RT "Glycosylphosphatidylinositol-anchored cell-surface proteins from
RT Arabidopsis.";
RL Electrophoresis 20:2027-2035(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP PROTEIN SEQUENCE OF 22-40, HYDROXYLATION AT PRO-24; PRO-26; PRO-28; PRO-32;
RP PRO-33; PRO-34; PRO-37; PRO-38 AND PRO-39, PYROGLUTAMATE FORMATION AT
RP GLN-22, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11006345; DOI=10.2307/3871187;
RA Schultz C.J., Johnson K.L., Currie G., Bacic A.;
RT "The classical arabinogalactan protein gene family of Arabidopsis.";
RL Plant Cell 12:1751-1767(2000).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12177459; DOI=10.1104/pp.003459;
RA Schultz C.J., Rumsewicz M.P., Johnson K.L., Jones B.J., Gaspar Y.M.,
RA Bacic A.;
RT "Using genomic resources to guide research directions. The arabinogalactan
RT protein gene family as a test case.";
RL Plant Physiol. 129:1448-1463(2002).
CC -!- FUNCTION: Proteoglycan that seems to be implicated in diverse
CC developmental roles such as differentiation, cell-cell recognition,
CC embryogenesis and programmed cell death.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots, flowers and leaves.
CC {ECO:0000269|PubMed:11006345}.
CC -!- PTM: O-glycosylated on hydroxyprolines; noncontiguous hydroxylproline
CC residues are glycosylated with arabinogalactan.
CC -!- SIMILARITY: Belongs to the classical AGP family. {ECO:0000305}.
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DR EMBL; AF082301; AAC77826.1; -; mRNA.
DR EMBL; AF060874; AAD38870.1; -; mRNA.
DR EMBL; AL353995; CAB89400.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91539.1; -; Genomic_DNA.
DR EMBL; AF372885; AAK49601.1; -; mRNA.
DR EMBL; AY042794; AAK68734.1; -; mRNA.
DR EMBL; BT002666; AAO11582.1; -; mRNA.
DR PIR; T49996; T49996.
DR RefSeq; NP_196605.1; NM_121081.3.
DR AlphaFoldDB; Q9ZT16; -.
DR STRING; 3702.AT5G10430.1; -.
DR PaxDb; Q9ZT16; -.
DR EnsemblPlants; AT5G10430.1; AT5G10430.1; AT5G10430.
DR GeneID; 830907; -.
DR Gramene; AT5G10430.1; AT5G10430.1; AT5G10430.
DR KEGG; ath:AT5G10430; -.
DR Araport; AT5G10430; -.
DR TAIR; locus:2142524; AT5G10430.
DR eggNOG; ENOG502SY2B; Eukaryota.
DR HOGENOM; CLU_149596_0_0_1; -.
DR InParanoid; Q9ZT16; -.
DR OMA; APHADRT; -.
DR PRO; PR:Q9ZT16; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9ZT16; baseline and differential.
DR Genevisible; Q9ZT16; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010198; P:synergid death; IMP:TAIR.
DR InterPro; IPR044959; AGP.
DR PANTHER; PTHR36321; PTHR36321; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW Hydroxylation; Lipoprotein; Membrane; Proteoglycan;
KW Pyrrolidone carboxylic acid; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:11006345"
FT CHAIN 22..111
FT /note="Classical arabinogalactan protein 4"
FT /id="PRO_0000268991"
FT PROPEP 112..135
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000268992"
FT REGION 22..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..97
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 24
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 26
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 28
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 32
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 33
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 34
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 37
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 38
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT MOD_RES 39
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:11006345"
FT LIPID 111
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 26
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 28
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 32
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 33
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 34
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 37
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 38
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
FT CARBOHYD 39
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000255"
SQ SEQUENCE 135 AA; 12803 MW; E2F4095EFB5BDBC3 CRC64;
MGSKIVQVFL MLALFATSAL AQAPAPTPTA TPPPATPPPV ATPPPVATPP PAATPAPATP
PPAATPAPAT TPPSVAPSPA DVPTASPPAP EGPTVSPSSA PGPSDASPAP SAAFSNKAFF
AGTAFAAIMY AAVLA
