ID   ERF1_CAEEL              Reviewed;         593 AA.
AC   O16520; S6EZT6;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   05-DEC-2018, sequence version 3.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=Eukaryotic peptide chain release factor subunit 1 {ECO:0000250|UniProtKB:P62495};
DE            Short=Eukaryotic release factor 1 {ECO:0000250|UniProtKB:P62495};
DE            Short=eRF1 {ECO:0000250|UniProtKB:P62495};
GN   Name=erfa-1 {ECO:0000312|WormBase:T05H4.6a};
GN   Synonyms=etf-1 {ECO:0000312|WormBase:T05H4.6a};
GN   ORFNames=T05H4.6 {ECO:0000312|WormBase:T05H4.6a};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Directs the termination of nascent peptide synthesis
CC       (translation) in response to the termination codons UAA, UAG and UGA.
CC       {ECO:0000250|UniProtKB:P62495}.
CC   -!- SUBUNIT: Heterodimer of two subunits, one of which binds GTP.
CC       {ECO:0000250|UniProtKB:P62495}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P62495}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a {ECO:0000312|WormBase:T05H4.6a};
CC         IsoId=O16520-1; Sequence=Displayed;
CC       Name=b {ECO:0000312|WormBase:T05H4.6b};
CC         IsoId=O16520-2; Sequence=VSP_059940;
CC   -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family.
CC       {ECO:0000305}.
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DR   EMBL; BX284605; CCD72044.1; -; Genomic_DNA.
DR   EMBL; BX284605; CDG24149.1; -; Genomic_DNA.
DR   PIR; T31907; T31907.
DR   RefSeq; NP_001294675.1; NM_001307746.1. [O16520-2]
DR   AlphaFoldDB; O16520; -.
DR   SMR; O16520; -.
DR   BioGRID; 44074; 13.
DR   IntAct; O16520; 1.
DR   STRING; 6239.T05H4.6a; -.
DR   EPD; O16520; -.
DR   PaxDb; O16520; -.
DR   PeptideAtlas; O16520; -.
DR   EnsemblMetazoa; T05H4.6a.1; T05H4.6a.1; WBGene00020269. [O16520-1]
DR   EnsemblMetazoa; T05H4.6b.1; T05H4.6b.1; WBGene00020269. [O16520-2]
DR   EnsemblMetazoa; T05H4.6b.2; T05H4.6b.2; WBGene00020269. [O16520-2]
DR   GeneID; 179028; -.
DR   CTD; 179028; -.
DR   WormBase; T05H4.6a; CE52351; WBGene00020269; erfa-1. [O16520-1]
DR   WormBase; T05H4.6b; CE13279; WBGene00020269; erfa-1. [O16520-2]
DR   eggNOG; KOG0688; Eukaryota.
DR   GeneTree; ENSGT00390000009004; -.
DR   HOGENOM; CLU_035759_0_0_1; -.
DR   InParanoid; O16520; -.
DR   PhylomeDB; O16520; -.
DR   Reactome; R-CEL-72764; Eukaryotic Translation Termination.
DR   Reactome; R-CEL-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR   Reactome; R-CEL-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   PRO; PR:O16520; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00020269; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0018444; C:translation release factor complex; IBA:GO_Central.
DR   GO; GO:1990825; F:sequence-specific mRNA binding; IBA:GO_Central.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IBA:GO_Central.
DR   GO; GO:0002184; P:cytoplasmic translational termination; IBA:GO_Central.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.30.420.60; -; 1.
DR   Gene3D; 3.30.960.10; -; 1.
DR   InterPro; IPR042226; eFR1_2_sf.
DR   InterPro; IPR005140; eRF1_1_Pelota.
DR   InterPro; IPR024049; eRF1_1_sf.
DR   InterPro; IPR005141; eRF1_2.
DR   InterPro; IPR005142; eRF1_3.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004403; Peptide_chain-rel_eRF1/aRF1.
DR   PANTHER; PTHR10113; PTHR10113; 1.
DR   Pfam; PF03463; eRF1_1; 1.
DR   Pfam; PF03464; eRF1_2; 1.
DR   Pfam; PF03465; eRF1_3; 1.
DR   SMART; SM01194; eRF1_1; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF55481; SSF55481; 1.
DR   TIGRFAMs; TIGR03676; aRF1/eRF1; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Cytoplasm; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..593
FT                   /note="Eukaryotic peptide chain release factor subunit 1"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000143148"
FT   VAR_SEQ         444..593
FT                   /note="Missing (in isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_059940"
SQ   SEQUENCE   593 AA;  66117 MW;  0C2532748F31E016 CRC64;
     MSAPAAGATA GGDDAADRNV EMWKIKRLIK SLELARGNGT SMISLIIPPK DQVARIQRML
     AEEYGTASNI KSRVNRLSVL GAITSVQGRL KLYNKVPPNG LVVYCGTIMT DEGKEKKVNI
     DFEPFKAINT SLYLCDNKFH TEALQGLLAD DNKFGFIIMD GNGCLFGTLQ GNTREVLHKF
     TVDLPKKHGR GGQSAVRFAR LRNEKRHNYV RKVAENSVEQ FIKNDKVTVA GLILAGSADF
     KTELGQSDMF DQRLQAKMIK TVDIAYGGEN GFNQAIELAA DTLASVKFIQ EKKLIGGYFD
     EISQDTGKYV FGVKDTLAAL EMGAIETLIC WENLDIVRYK MKNSLGEDIL LNLRPDEEKD
     KSHFTDSETG QDMEIIETMP LLEWFANNYK NFGAALEIVT DKSQEGAQFV RGFGGIGGLL
     RYRVDLAHVD LEDELDNIDL DDYSRAAVVS HWAPYSFLLL FKLVSSFFFI NLFINYPALS
     NYNISLSQSS SSLIAISHTT LYSKFLFHFI EFAPRVPHYC RFVWSVDAFS SAGKASHVCQ
     HTEKETFHAR NEDAIGGLFN RTKVEARATH SHSMTSTLSL PSVDVSTRNS NDF