ERF1_DROME
ID ERF1_DROME Reviewed; 438 AA.
AC Q9VPH7; A4V259; Q0E8C7; Q8T071;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Eukaryotic peptide chain release factor subunit 1;
DE Short=Eukaryotic release factor 1;
DE Short=eRF1;
GN Name=eRF1; ORFNames=CG5605;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Directs the termination of nascent peptide synthesis
CC (translation) in response to the termination codons UAA, UAG and UGA.
CC {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of two subunits, one of which binds GTP.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family.
CC {ECO:0000305}.
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DR EMBL; AE014296; AAF51574.2; -; Genomic_DNA.
DR EMBL; AE014296; AAN12121.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN12122.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN12123.1; -; Genomic_DNA.
DR EMBL; AE014296; AAO41278.1; -; Genomic_DNA.
DR EMBL; AY069511; AAL39656.1; -; mRNA.
DR RefSeq; NP_649210.1; NM_140953.3.
DR RefSeq; NP_730517.1; NM_168845.2.
DR RefSeq; NP_730518.1; NM_168846.2.
DR RefSeq; NP_730519.1; NM_168847.2.
DR RefSeq; NP_730520.1; NM_168848.2.
DR RefSeq; NP_788547.1; NM_176369.3.
DR AlphaFoldDB; Q9VPH7; -.
DR SMR; Q9VPH7; -.
DR BioGRID; 65501; 10.
DR DIP; DIP-18487N; -.
DR IntAct; Q9VPH7; 4.
DR STRING; 7227.FBpp0304202; -.
DR iPTMnet; Q9VPH7; -.
DR PaxDb; Q9VPH7; -.
DR PRIDE; Q9VPH7; -.
DR DNASU; 40240; -.
DR EnsemblMetazoa; FBtr0078173; FBpp0077831; FBgn0036974.
DR EnsemblMetazoa; FBtr0078174; FBpp0077832; FBgn0036974.
DR EnsemblMetazoa; FBtr0078175; FBpp0077833; FBgn0036974.
DR EnsemblMetazoa; FBtr0078176; FBpp0077834; FBgn0036974.
DR EnsemblMetazoa; FBtr0078177; FBpp0077835; FBgn0036974.
DR EnsemblMetazoa; FBtr0078178; FBpp0077836; FBgn0036974.
DR GeneID; 40240; -.
DR KEGG; dme:Dmel_CG5605; -.
DR CTD; 40240; -.
DR FlyBase; FBgn0036974; eRF1.
DR VEuPathDB; VectorBase:FBgn0036974; -.
DR eggNOG; KOG0688; Eukaryota.
DR GeneTree; ENSGT00390000009004; -.
DR InParanoid; Q9VPH7; -.
DR OMA; GQEMEVV; -.
DR OrthoDB; 592406at2759; -.
DR PhylomeDB; Q9VPH7; -.
DR Reactome; R-DME-72764; Eukaryotic Translation Termination.
DR Reactome; R-DME-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 40240; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 40240; -.
DR PRO; PR:Q9VPH7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036974; Expressed in cleaving embryo and 21 other tissues.
DR ExpressionAtlas; Q9VPH7; baseline and differential.
DR Genevisible; Q9VPH7; DM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0018444; C:translation release factor complex; IBA:GO_Central.
DR GO; GO:1990825; F:sequence-specific mRNA binding; IBA:GO_Central.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IBA:GO_Central.
DR GO; GO:0002184; P:cytoplasmic translational termination; IBA:GO_Central.
DR GO; GO:0006415; P:translational termination; IMP:FlyBase.
DR Gene3D; 3.30.1330.30; -; 1.
DR Gene3D; 3.30.420.60; -; 1.
DR Gene3D; 3.30.960.10; -; 1.
DR InterPro; IPR042226; eFR1_2_sf.
DR InterPro; IPR005140; eRF1_1_Pelota.
DR InterPro; IPR024049; eRF1_1_sf.
DR InterPro; IPR005141; eRF1_2.
DR InterPro; IPR005142; eRF1_3.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004403; Peptide_chain-rel_eRF1/aRF1.
DR PANTHER; PTHR10113; PTHR10113; 1.
DR Pfam; PF03463; eRF1_1; 1.
DR Pfam; PF03464; eRF1_2; 1.
DR Pfam; PF03465; eRF1_3; 1.
DR SMART; SM01194; eRF1_1; 1.
DR SUPFAM; SSF55315; SSF55315; 1.
DR SUPFAM; SSF55481; SSF55481; 1.
DR TIGRFAMs; TIGR03676; aRF1/eRF1; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Protein biosynthesis; Reference proteome.
FT CHAIN 1..438
FT /note="Eukaryotic peptide chain release factor subunit 1"
FT /id="PRO_0000143153"
FT MOD_RES 341
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 438 AA; 49160 MW; E16B3C08FBA1C485 CRC64;
MSGEETSADR NVEIWKIKKL IKSLEMARGN GTSMISLIIP PKDQISRVSK MLADEFGTAS
NIKSRVNRLS VLGAITSVQH RLKLYTKVPP NGLVIYCGTI VTEEGKEKKV NIDFEPFKPI
NTSLYLCDNK FHTEALTALL ADDNKFGFIV MDGNGALFGT LQGNTREVLH KFTVDLPKKH
GRGGQSALRF ARLRMEKRHN YVRKVAEVAT QLFITNDKPN IAGLILAGSA DFKTELSQSD
MFDPRLQSKV IKLVDVSYGG ENGFNQAIEL AAESLQNVKF IQEKKLIGRY FDEISQDTGK
YCFGVEDTLR ALELGSVETL ICWENLDIQR YVLKNHANST STTVLHLTPE QEKDKSHFTD
KESGVEMELI ESQPLLEWLA NNYKMFGATL EIITDKSQEG SQFVRGFGGI GGILRYKVDF
QSMQLDELDN DGFDLDDY
