ERF1_RAT
ID ERF1_RAT Reviewed; 437 AA.
AC Q5U2Q7;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Eukaryotic peptide chain release factor subunit 1;
DE Short=Eukaryotic release factor 1;
DE Short=eRF1;
GN Name=Etf1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Directs the termination of nascent peptide synthesis
CC (translation) in response to the termination codons UAA, UAG and UGA
CC (By similarity). Component of the transient SURF complex which recruits
CC UPF1 to stalled ribosomes in the context of nonsense-mediated decay
CC (NMD) of mRNAs containing premature stop codons (By similarity).
CC {ECO:0000250|UniProtKB:P62495}.
CC -!- SUBUNIT: Heterodimer of two subunits, one of which binds GTP (By
CC similarity). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3)
CC complex (By similarity). Interacts with JMJD4 (By similarity). The
CC ETF1-GSPT1 complex interacts with JMJD4 (By similarity).
CC {ECO:0000250|UniProtKB:P62495}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P62495}.
CC -!- PTM: Hydroxylation at Lys-63 by JMJD4 promotes its translational
CC termination efficiency. {ECO:0000250|UniProtKB:P62495}.
CC -!- PTM: Methylated at Gln-185 by N6AMT1. {ECO:0000250|UniProtKB:P62495}.
CC -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family.
CC {ECO:0000305}.
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DR EMBL; BC085902; AAH85902.1; -; mRNA.
DR RefSeq; NP_001008345.1; NM_001008344.1.
DR AlphaFoldDB; Q5U2Q7; -.
DR SMR; Q5U2Q7; -.
DR BioGRID; 258740; 2.
DR IntAct; Q5U2Q7; 3.
DR STRING; 10116.ENSRNOP00000026398; -.
DR PhosphoSitePlus; Q5U2Q7; -.
DR jPOST; Q5U2Q7; -.
DR PaxDb; Q5U2Q7; -.
DR PRIDE; Q5U2Q7; -.
DR Ensembl; ENSRNOT00000026398; ENSRNOP00000026398; ENSRNOG00000019450.
DR GeneID; 307503; -.
DR KEGG; rno:307503; -.
DR UCSC; RGD:1305712; rat.
DR CTD; 2107; -.
DR RGD; 1305712; Etf1.
DR eggNOG; KOG0688; Eukaryota.
DR GeneTree; ENSGT00390000009004; -.
DR HOGENOM; CLU_035759_2_1_1; -.
DR InParanoid; Q5U2Q7; -.
DR OMA; RCHGENT; -.
DR OrthoDB; 592406at2759; -.
DR PhylomeDB; Q5U2Q7; -.
DR TreeFam; TF105672; -.
DR Reactome; R-RNO-72764; Eukaryotic Translation Termination.
DR Reactome; R-RNO-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-RNO-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR PRO; PR:Q5U2Q7; -.
DR Proteomes; UP000002494; Chromosome 18.
DR Bgee; ENSRNOG00000019450; Expressed in quadriceps femoris and 19 other tissues.
DR Genevisible; Q5U2Q7; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0018444; C:translation release factor complex; ISO:RGD.
DR GO; GO:1990825; F:sequence-specific mRNA binding; ISO:RGD.
DR GO; GO:0003747; F:translation release factor activity; ISO:RGD.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IBA:GO_Central.
DR GO; GO:0008079; F:translation termination factor activity; ISS:UniProtKB.
DR GO; GO:0002184; P:cytoplasmic translational termination; IBA:GO_Central.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0006479; P:protein methylation; ISO:RGD.
DR GO; GO:0006449; P:regulation of translational termination; ISO:RGD.
DR GO; GO:0006415; P:translational termination; ISO:RGD.
DR Gene3D; 3.30.1330.30; -; 1.
DR Gene3D; 3.30.420.60; -; 1.
DR Gene3D; 3.30.960.10; -; 1.
DR InterPro; IPR042226; eFR1_2_sf.
DR InterPro; IPR005140; eRF1_1_Pelota.
DR InterPro; IPR024049; eRF1_1_sf.
DR InterPro; IPR005141; eRF1_2.
DR InterPro; IPR005142; eRF1_3.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004403; Peptide_chain-rel_eRF1/aRF1.
DR PANTHER; PTHR10113; PTHR10113; 1.
DR Pfam; PF03463; eRF1_1; 1.
DR Pfam; PF03464; eRF1_2; 1.
DR Pfam; PF03465; eRF1_3; 1.
DR SMART; SM01194; eRF1_1; 1.
DR SUPFAM; SSF55315; SSF55315; 1.
DR SUPFAM; SSF55481; SSF55481; 1.
DR TIGRFAMs; TIGR03676; aRF1/eRF1; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Hydroxylation; Isopeptide bond; Methylation;
KW Nonsense-mediated mRNA decay; Phosphoprotein; Protein biosynthesis;
KW Reference proteome; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT CHAIN 2..437
FT /note="Eukaryotic peptide chain release factor subunit 1"
FT /id="PRO_0000143143"
FT MOTIF 61..64
FT /note="NIKS motif; plays an important role in translational
FT termination"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT MOD_RES 63
FT /note="4-hydroxylysine"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT MOD_RES 185
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT MOD_RES 347
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT CROSSLNK 87
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P62495"
FT CROSSLNK 404
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P62495"
SQ SEQUENCE 437 AA; 49031 MW; CECC50D100E59D19 CRC64;
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS
NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI
NTSLYLCDNK FHTEALTALL SDDSKFGFIV IDGSGALFGT LQGNTREVLH KFTVDLPKKH
GRGGQSALRF ARLRMEKRHN YVRKVAETAV QLFISGDKVN VAGLVLAGSA DFKTELSQSD
MFDQRLQSKV LKLVDISYGG ENGFNQAIEL STEVLSNVKF IQEKKLIGRY FDEISQDTGK
YCFGVEDTLK ALEMGAVEIL IVYENLDIMR YVLHCQGTEE EKILYLTPEQ EKDKSHFTDK
ETGQEHELIE SMPLLEWFAN NYKKFGATLE IVTDKSQEGS QFVKGFGGIG GILRYRVDFQ
GMEYQGGDDE FFDLDDY
