ID   ERF1_XENTR              Reviewed;         437 AA.
AC   P62498; Q28F03; Q6P843;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Eukaryotic peptide chain release factor subunit 1;
DE            Short=Eukaryotic release factor 1;
DE            Short=eRF1;
GN   Name=etf1; ORFNames=TGas126k19.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Directs the termination of nascent peptide synthesis
CC       (translation) in response to the termination codons UAA, UAG and UGA
CC       (By similarity). Component of the transient SURF complex which recruits
CC       UPF1 to stalled ribosomes in the context of nonsense-mediated decay
CC       (NMD) of mRNAs containing premature stop codons (By similarity).
CC       Required for SHFL-mediated translation termination which inhibits
CC       programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and
CC       cellular genes. {ECO:0000250|UniProtKB:P35615,
CC       ECO:0000250|UniProtKB:P62495}.
CC   -!- SUBUNIT: Heterodimer of two subunits, one of which binds GTP (By
CC       similarity). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3)
CC       complex (By similarity). {ECO:0000250|UniProtKB:P62495}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P62495}.
CC   -!- SIMILARITY: Belongs to the eukaryotic release factor 1 family.
CC       {ECO:0000305}.
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DR   EMBL; CR762244; CAJ81583.1; -; mRNA.
DR   EMBL; BC061387; AAH61387.1; -; mRNA.
DR   RefSeq; NP_989035.1; NM_203704.1.
DR   AlphaFoldDB; P62498; -.
DR   BMRB; P62498; -.
DR   SMR; P62498; -.
DR   PaxDb; P62498; -.
DR   DNASU; 394632; -.
DR   Ensembl; ENSXETT00000025940; ENSXETP00000025940; ENSXETG00000011876.
DR   GeneID; 394632; -.
DR   KEGG; xtr:394632; -.
DR   CTD; 2107; -.
DR   Xenbase; XB-GENE-5744577; etf1.
DR   eggNOG; KOG0688; Eukaryota.
DR   HOGENOM; CLU_035759_2_1_1; -.
DR   InParanoid; P62498; -.
DR   OrthoDB; 592406at2759; -.
DR   PhylomeDB; P62498; -.
DR   Reactome; R-XTR-72764; Eukaryotic Translation Termination.
DR   Reactome; R-XTR-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000011876; Expressed in ovary and 15 other tissues.
DR   ExpressionAtlas; P62498; baseline.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0018444; C:translation release factor complex; IBA:GO_Central.
DR   GO; GO:1990825; F:sequence-specific mRNA binding; IBA:GO_Central.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IBA:GO_Central.
DR   GO; GO:0008079; F:translation termination factor activity; ISS:UniProtKB.
DR   GO; GO:0002184; P:cytoplasmic translational termination; IBA:GO_Central.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR   GO; GO:0006449; P:regulation of translational termination; ISS:UniProtKB.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.30.420.60; -; 1.
DR   Gene3D; 3.30.960.10; -; 1.
DR   InterPro; IPR042226; eFR1_2_sf.
DR   InterPro; IPR005140; eRF1_1_Pelota.
DR   InterPro; IPR024049; eRF1_1_sf.
DR   InterPro; IPR005141; eRF1_2.
DR   InterPro; IPR005142; eRF1_3.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004403; Peptide_chain-rel_eRF1/aRF1.
DR   PANTHER; PTHR10113; PTHR10113; 1.
DR   Pfam; PF03463; eRF1_1; 1.
DR   Pfam; PF03464; eRF1_2; 1.
DR   Pfam; PF03465; eRF1_3; 1.
DR   SMART; SM01194; eRF1_1; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF55481; SSF55481; 1.
DR   TIGRFAMs; TIGR03676; aRF1/eRF1; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nonsense-mediated mRNA decay; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..437
FT                   /note="Eukaryotic peptide chain release factor subunit 1"
FT                   /id="PRO_0000143145"
FT   MOTIF           61..64
FT                   /note="NIKS motif; plays an important role in translational
FT                   termination"
FT                   /evidence="ECO:0000250|UniProtKB:P62495"
SQ   SEQUENCE   437 AA;  49022 MW;  F515A6381D03A91C CRC64;
     MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS
     NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI
     NTSLYLCDNK FHTEALTALL SDDSKFGFIV IDGSGALFGT LQGNTREVLH KFTVDLPKKH
     GRGGQSALRF ARLRMEKRHN YVRKVAETAV QLFISGDKVN VAGLVLAGSA DFKTELSQSD
     MFDQRLQSKV LKLVDISYGG ENGFNQAIEL STEVLSNVKF IQEKKLIGRY FDEISQDTGK
     YCFGVEDTLK ALEMGAVEIL IVYENLDIMR YVLRCNGSEE EKTLYLTPEQ EKDKSHFIDK
     ETGQEHELIE SMPLLEWFAN NYKKFGATLE IVTDKSQEGS QFVKGFGGIG GILRYRVDFQ
     GMEYQGGDDE FFDLDDY