ERF2_TOBAC
ID ERF2_TOBAC Reviewed; 233 AA.
AC Q40479;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Ethylene-responsive transcription factor 2;
DE Short=NtERF2;
DE AltName: Full=Ethylene-responsive element-binding factor 2;
DE Short=EREBP-2;
GN Name=ERF2; Synonyms=ERF-2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Bright Yellow 4; TISSUE=Leaf;
RX PubMed=7756828; DOI=10.2307/3869993;
RA Ohme-Takagi M., Shinshi H.;
RT "Ethylene-inducible DNA binding proteins that interact with an ethylene
RT responsive element.";
RL Plant Cell 7:173-182(1995).
RN [2]
RP FUNCTION, AND INDUCTION.
RX DOI=10.1046/j.1365-313x.1998.00243.x;
RA Suzuki K., Suzuki N., Ohme-Takagi M., Shinshi H.;
RT "Immediate early induction of mRNAs for ethylene-responsive transcription
RT factors in tobacco leaf strips after cutting.";
RL Plant J. 15:657-665(1998).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=10652129; DOI=10.1046/j.1365-313x.1999.00634.x;
RA Yamamoto S., Suzuki K., Shinshi H.;
RT "Elicitor-responsive, ethylene-independent activation of GCC box-mediated
RT transcription that is regulated by both protein phosphorylation and
RT dephosphorylation in cultured tobacco cells.";
RL Plant J. 20:571-579(1999).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10792818; DOI=10.1046/j.1365-313x.2000.00709.x;
RA Ohta M., Ohme-Takagi M., Shinshi H.;
RT "Three ethylene-responsive transcription factors in tobacco with distinct
RT transactivation functions.";
RL Plant J. 22:29-38(2000).
RN [5]
RP INDUCTION.
RX PubMed=12090623; DOI=10.1023/a:1015553232309;
RA Nishiuchi T., Suzuki K., Kitajima S., Sato F., Shinshi H.;
RT "Wounding activates immediate early transcription of genes for ERFs in
RT tobacco plants.";
RL Plant Mol. Biol. 49:473-482(2002).
CC -!- FUNCTION: Acts as a transcriptional activator and may be involved in
CC disease resistance pathways. Binds to the GCC-box pathogenesis-related
CC promoter element. Involved in the regulation of gene expression by
CC stress factors and by components of stress signal transduction pathways
CC mediated by ethylene, that seems to depend on a protein kinase cascade,
CC and to be influenced by methyl-jasmonate. {ECO:0000269|PubMed:10652129,
CC ECO:0000269|PubMed:10792818, ECO:0000269|PubMed:7756828,
CC ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366,
CC ECO:0000269|PubMed:10792818}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and at low levels in buds and
CC leaves. Not expressed in cell culture. {ECO:0000269|PubMed:7756828}.
CC -!- INDUCTION: Induced by ethephon (ethylene-releasing compound) in buds
CC and to a lower extent in leaves. Strongly induced by cycloheximide and
CC mechanical stimuli. Wounding leads to a both local and systemic
CC expression, independently of ethylene, and through a de-novo-protein-
CC synthesis-independent regulation. Wound induction is reduced by methyl-
CC jasmonate. Induction by purified xylanase from Trichoderma viride (TvX)
CC and another elicitor from Phytophthora infestans (PiE), that appears to
CC be mediated by both protein kinases and protein phosphatases.
CC {ECO:0000269|PubMed:10652129, ECO:0000269|PubMed:12090623,
CC ECO:0000269|PubMed:7756828, ECO:0000269|Ref.2}.
CC -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC motif. The affinity of this binding is higher if the seventh amino-acid
CC of this domain is basic (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ethylene-response factor family. Class 1
CC subfamily. {ECO:0000305}.
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DR EMBL; D38126; BAA07324.1; -; mRNA.
DR PIR; T02590; T02590.
DR RefSeq; NP_001311965.1; NM_001325036.1.
DR AlphaFoldDB; Q40479; -.
DR SMR; Q40479; -.
DR STRING; 4097.Q40479; -.
DR GeneID; 107769743; -.
DR KEGG; nta:107769743; -.
DR OMA; WGDLPFK; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR044808; ERF_plant.
DR PANTHER; PTHR31190; PTHR31190; 1.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..233
FT /note="Ethylene-responsive transcription factor 2"
FT /id="PRO_0000112548"
FT DNA_BIND 98..156
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 164..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 233 AA; 25563 MW; 6CD16783582C0CB5 CRC64;
MYQPISTELP PTSFSSLMPC LTDTWGDLPL KVDDSEDMVI YGLLSDALTA GWTPFNLTST
EIKAEPREEI EPATIPVPSV APPAETTTAQ AVVPKGRHYR GVRQRPWGKF AAEIRDPAKN
GARVWLGTYE TAEEAALAYD KAAYRMRGSK ALLNFPHRIG LNEPEPVRLT AKRRSPEPAS
SSISSALENG SPKRRRKAVA AKKAELEVQS RSNAMQVGCQ MEQFPVGEQL LVS
