ID   ERF3_TOBAC              Reviewed;         225 AA.
AC   Q9SXS8;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Ethylene-responsive transcription factor 3;
DE   AltName: Full=Ethylene-responsive element-binding factor 3 homolog;
DE   AltName: Full=Ethylene-responsive element-binding factor 5;
DE            Short=EREBP-5;
DE   AltName: Full=NtERF5;
GN   Name=ERF3; Synonyms=ERF-5, ERF5;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10902919; DOI=10.3109/10425170009033979;
RA   Ashida Y., Yokobatake N., Kohchi C., Shimoda K., Hirata T.;
RT   "Cloning of cDNA encoding ethylene-responsive element binding protein-5 in
RT   the cultured cells of Nicotiana tabacum.";
RL   DNA Seq. 11:125-129(2000).
CC   -!- FUNCTION: Transcription factor that binds to the GCC-box pathogenesis-
CC       related promoter element. Involved in the regulation of gene expression
CC       by stress factors and by components of stress signal transduction
CC       pathways. Probably acts as a transcriptional repressor and may regulate
CC       other AtERFs (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC       motif. The affinity of this binding is higher if the seventh amino-acid
CC       of this domain is basic (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC       repression (EAR) motif, which may be involved in the activity of
CC       transcriptional repression. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ethylene-response factor family. Class 2
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was named ERF5 but it corresponds to Arabidopsis ERF3.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB024575; BAA76734.1; -; mRNA.
DR   RefSeq; NP_001312653.1; NM_001325724.1.
DR   AlphaFoldDB; Q9SXS8; -.
DR   SMR; Q9SXS8; -.
DR   STRING; 4097.Q9SXS8; -.
DR   GeneID; 107802932; -.
DR   KEGG; nta:107802932; -.
DR   OMA; DEDLQCT; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd00018; AP2; 1.
DR   Gene3D; 3.30.730.10; -; 1.
DR   InterPro; IPR001471; AP2/ERF_dom.
DR   InterPro; IPR036955; AP2/ERF_dom_sf.
DR   InterPro; IPR016177; DNA-bd_dom_sf.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   SMART; SM00380; AP2; 1.
DR   SUPFAM; SSF54171; SSF54171; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..225
FT                   /note="Ethylene-responsive transcription factor 3"
FT                   /id="PRO_0000112553"
FT   DNA_BIND        27..84
FT                   /note="AP2/ERF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          82..193
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           202..208
FT                   /note="EAR-like (transcriptional repression)"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        93..134
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        169..188
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   225 AA;  24714 MW;  1347A2CFD0C58CB7 CRC64;
     MRRGRAAAAP APVTGEPNGS GGSKEIRFRG VRKRPWGRFA AEIRDPWKKT RVWLGTFDSA
     EDAARAYDAA ARALRGPKAK TNFPLPYAHH HQFNQGHNPN NDPFVDSRFY PQDNPIISQR
     PTSSSMSSTV ESFSGPRPPP APRQQTTASS RKYTRSPPVV PDDCHSDCDS SSSVVDHGDC
     EKENDNDNDN IASSSFRKPL LFDLNLPPPM DDAGADDLHC TALCL