ID   ERF4_NICSY              Reviewed;         227 AA.
AC   Q9LW49;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Ethylene-responsive transcription factor 4;
DE   AltName: Full=Ethylene-responsive element-binding factor 3;
DE            Short=EREBP-3;
DE   AltName: Full=Ethylene-responsive element-binding factor 4 homolog;
DE   AltName: Full=NsERF3;
GN   Name=ERF4; Synonyms=ERF-3, ERF3;
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND TISSUE SPECIFICITY.
RX   PubMed=10945353; DOI=10.1093/pcp/41.6.817;
RA   Kitajima S., Koyama T., Ohme-Takagi M., Shinshi H., Sato F.;
RT   "Characterization of gene expression of NsERFs, transcription factors of
RT   basic PR genes from Nicotiana sylvestris.";
RL   Plant Cell Physiol. 41:817-824(2000).
CC   -!- FUNCTION: Transcription factor that binds to the GCC-box pathogenesis-
CC       related promoter element. Involved in the regulation of gene expression
CC       by stress factors and by components of stress signal transduction
CC       pathways. Probably acts as a transcriptional repressor and may regulate
CC       other AtERFs (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, mostly in root tip, lateral
CC       root tips, cortex and vascular tissues. {ECO:0000269|PubMed:10945353}.
CC   -!- INDUCTION: Strongly and quickly induced by ethylene.
CC       {ECO:0000269|PubMed:10945353}.
CC   -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC       motif. The affinity of this binding is higher if the seventh amino-acid
CC       of this domain is basic (By similarity). {ECO:0000250}.
CC   -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC       repression (EAR) motif, which may be involved in the activity of
CC       transcriptional repression. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ethylene-response factor family. Class 2
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was named ERF3 but it corresponds to Arabidopsis ERF4.
CC       {ECO:0000305}.
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DR   EMBL; AB016265; BAA97123.1; -; Genomic_DNA.
DR   RefSeq; XP_009772525.1; XM_009774223.1.
DR   AlphaFoldDB; Q9LW49; -.
DR   SMR; Q9LW49; -.
DR   STRING; 4096.XP_009772525.1; -.
DR   GeneID; 104222902; -.
DR   KEGG; nsy:104222902; -.
DR   eggNOG; ENOG502RYHH; Eukaryota.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR   CDD; cd00018; AP2; 1.
DR   Gene3D; 3.30.730.10; -; 1.
DR   InterPro; IPR001471; AP2/ERF_dom.
DR   InterPro; IPR036955; AP2/ERF_dom_sf.
DR   InterPro; IPR016177; DNA-bd_dom_sf.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   SMART; SM00380; AP2; 1.
DR   SUPFAM; SSF54171; SSF54171; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..227
FT                   /note="Ethylene-responsive transcription factor 4"
FT                   /id="PRO_0000112555"
FT   DNA_BIND        26..83
FT                   /note="AP2/ERF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          74..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           217..223
FT                   /note="EAR-like (transcriptional repression)"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        82..103
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   227 AA;  24626 MW;  42199D4B38CCFEE3 CRC64;
     MAVKNKVSNG DLKGGNVKTN GVKEVHYRGV RKRPWGRYAA EIRDPGKKSR VWLGTFDTAE
     EAAKAYDTAA REFRGPKAKT NFPLPSENQS TSHSSTMESS SGETGIHAPP HAPLELDLTR
     RLGSVAADGG DNCRRSGEVG YPIFHQQPTV AVLPNGQPVL LFDSLWRPGV VNRPQPYHVM
     PMAMGFNGVN AGVDPTVSDS SSVVEENQYD GKRGIDLDLN LAPPTEF