ERF53_ARATH
ID ERF53_ARATH Reviewed; 336 AA.
AC Q9SKT1;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Ethylene-responsive transcription factor ERF053 {ECO:0000305};
DE Short=AtERF53 {ECO:0000303|PubMed:22095047};
GN Name=ERF053 {ECO:0000303|PubMed:16407444};
GN OrderedLocusNames=At2g20880 {ECO:0000312|Araport:AT2G20880};
GN ORFNames=F5H14.15 {ECO:0000312|EMBL:AAD20907.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
RN [5]
RP INDUCTION BY DROUGHT.
RX PubMed=18550687; DOI=10.1104/pp.108.116632;
RA Endo A., Sawada Y., Takahashi H., Okamoto M., Ikegami K., Koiwai H.,
RA Seo M., Toyomasu T., Mitsuhashi W., Shinozaki K., Nakazono M., Kamiya Y.,
RA Koshiba T., Nambara E.;
RT "Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs
RT in vascular parenchyma cells.";
RL Plant Physiol. 147:1984-1993(2008).
RN [6]
RP FUNCTION, INTERACTION WITH RGLG1 AND RGLG2, SUBCELLULAR LOCATION,
RP INDUCTION, AND UBIQUITINATION.
RX PubMed=22095047; DOI=10.1104/pp.111.189738;
RA Cheng M.C., Hsieh E.J., Chen J.H., Chen H.Y., Lin T.P.;
RT "Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53
RT and negatively regulates the plant drought stress response.";
RL Plant Physiol. 158:363-375(2012).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=23625358; DOI=10.1007/s11103-013-0054-z;
RA Hsieh E.J., Cheng M.C., Lin T.P.;
RT "Functional characterization of an abiotic stress-inducible transcription
RT factor AtERF53 in Arabidopsis thaliana.";
RL Plant Mol. Biol. 82:223-237(2013).
CC -!- FUNCTION: Transcriptional activator involved in abiotic stress
CC tolerance. Can directly regulate stress-related gene expression by
CC binding to the DNA sequence 5'-[AG]CCGAC-3' (DRE element) of their
CC promoter region. Involved in the regulation of stomatal closure
CC movement under drought stress (PubMed:23625358). Acts as a positive
CC regulator of drought stress response (PubMed:22095047).
CC {ECO:0000269|PubMed:22095047, ECO:0000269|PubMed:23625358}.
CC -!- SUBUNIT: Interacts with RGLG1 and RGLG2. {ECO:0000269|PubMed:22095047}.
CC -!- INTERACTION:
CC Q9SKT1; Q94ID6: ERF12; NbExp=3; IntAct=EBI-25511859, EBI-4446727;
CC Q9SKT1; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-25511859, EBI-2000137;
CC Q9SKT1; Q9FE67: ERF9; NbExp=3; IntAct=EBI-25511859, EBI-4431933;
CC Q9SKT1; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-25511859, EBI-3946459;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22095047}.
CC -!- INDUCTION: Induced by drought stress (PubMed:18550687, PubMed:22095047,
CC PubMed:23625358). Induced by salt stress (PubMed:22095047). Induced by
CC abscisic acid (ABA) and heat stress (PubMed:23625358).
CC {ECO:0000269|PubMed:18550687, ECO:0000269|PubMed:22095047,
CC ECO:0000269|PubMed:23625358}.
CC -!- PTM: Ubiquitinated by RGLG2. Ubiquitination of ERF053 leads to its
CC degradation by the proteasome. {ECO:0000269|PubMed:22095047}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AC006234; AAD20907.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07091.1; -; Genomic_DNA.
DR EMBL; AY062843; AAL32921.1; -; mRNA.
DR EMBL; AY081659; AAM10221.1; -; mRNA.
DR PIR; E84594; E84594.
DR RefSeq; NP_179685.1; NM_127658.4.
DR AlphaFoldDB; Q9SKT1; -.
DR SMR; Q9SKT1; -.
DR BioGRID; 1975; 7.
DR IntAct; Q9SKT1; 4.
DR STRING; 3702.AT2G20880.1; -.
DR iPTMnet; Q9SKT1; -.
DR PaxDb; Q9SKT1; -.
DR PRIDE; Q9SKT1; -.
DR ProteomicsDB; 220560; -.
DR EnsemblPlants; AT2G20880.1; AT2G20880.1; AT2G20880.
DR GeneID; 816622; -.
DR Gramene; AT2G20880.1; AT2G20880.1; AT2G20880.
DR KEGG; ath:AT2G20880; -.
DR Araport; AT2G20880; -.
DR TAIR; locus:2051359; AT2G20880.
DR eggNOG; ENOG502QU46; Eukaryota.
DR HOGENOM; CLU_047640_0_0_1; -.
DR InParanoid; Q9SKT1; -.
DR OMA; HQKQCET; -.
DR OrthoDB; 1049374at2759; -.
DR PhylomeDB; Q9SKT1; -.
DR PRO; PR:Q9SKT1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKT1; baseline and differential.
DR Genevisible; Q9SKT1; AT.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:1900036; P:positive regulation of cellular response to heat; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:2000070; P:regulation of response to water deprivation; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..336
FT /note="Ethylene-responsive transcription factor ERF053"
FT /id="PRO_0000290393"
FT DNA_BIND 186..243
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 247..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 336 AA; 38398 MW; 258775B240ECBE19 CRC64;
MATAKNKGKS IRVLGTSEAE KKDEMELEEE FQFSSGKYKD SGPGSDMWLG DASSTSPRSL
RKTRTFDRHN PYLVSSYATP QPPTTTTCSV SFPFYLPPAI QNQQRFLHPN DPSGQRQQQM
ISFDPQQQVQ PYVAQQQQQQ QHLLQYWRDI LKLSPSGRMM MMNMLRQESD LPLTRPPVQP
FSATKLYRGV RQRHWGKWVA EIRKPRNRTR LWLGTFDTAE EAAMAYDREA FKLRGETARL
NFPELFLNKQ EPTPVHQKQC ETGTTSEDSS RRGEDDSSTA LAVGGVSEET GWAEAWFNAI
PEEWGPGSPL WDDYHFPISN HKDDLDATQN SSSDTI
