ERF81_ARATH
ID ERF81_ARATH Reviewed; 189 AA.
AC Q94ID6; Q9SGN3;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Ethylene-responsive transcription factor 12;
DE Short=AtERF12;
DE AltName: Full=Ethylene-responsive element-binding factor 12;
DE Short=EREBP-12;
GN Name=ERF12; Synonyms=ERF-12, ERF081; OrderedLocusNames=At1g28360;
GN ORFNames=F3M18.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DOMAIN.
RX PubMed=11487705; DOI=10.2307/3871331;
RA Ohta M., Matsui K., Hiratsu K., Shinshi H., Ohme-Takagi M.;
RT "Repression domains of class II ERF transcriptional repressors share an
RT essential motif for active repression.";
RL Plant Cell 13:1959-1968(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Involved in the regulation of gene expression by stress
CC factors and by components of stress signal transduction pathways.
CC Transcription factor that binds to the GCC-box pathogenesis-related
CC promoter element. Acts as a transcriptional inhibitor and may regulate
CC other AtERFs (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:11487705}.
CC -!- INTERACTION:
CC Q94ID6; Q9FIW5: ANAC094; NbExp=3; IntAct=EBI-4446727, EBI-25522986;
CC Q94ID6; Q84K51: At1g77770; NbExp=3; IntAct=EBI-4446727, EBI-25520665;
CC Q94ID6; Q38897: BEL1; NbExp=3; IntAct=EBI-4446727, EBI-1153783;
CC Q94ID6; O80513: CYCU4-1; NbExp=3; IntAct=EBI-4446727, EBI-1773819;
CC Q94ID6; Q9SKT1: ERF053; NbExp=3; IntAct=EBI-4446727, EBI-25511859;
CC Q94ID6; Q9FYK5: ESR2; NbExp=4; IntAct=EBI-4446727, EBI-1536925;
CC Q94ID6; Q8L8A6: GRF5; NbExp=3; IntAct=EBI-4446727, EBI-1396652;
CC Q94ID6; Q9AT61: LBD13; NbExp=5; IntAct=EBI-4446727, EBI-4432427;
CC Q94ID6; Q9SHE9: LBD4; NbExp=3; IntAct=EBI-4446727, EBI-4445715;
CC Q94ID6; P94077: LSD1; NbExp=3; IntAct=EBI-4446727, EBI-5849461;
CC Q94ID6; Q9FIR9: LSU2; NbExp=4; IntAct=EBI-4446727, EBI-4424076;
CC Q94ID6; Q9LSI4: MGH6.1; NbExp=3; IntAct=EBI-4446727, EBI-15198743;
CC Q94ID6; Q9LK95: MYB21; NbExp=3; IntAct=EBI-4446727, EBI-15197631;
CC Q94ID6; Q9SFD8: NFYB9; NbExp=3; IntAct=EBI-4446727, EBI-2475789;
CC Q94ID6; O82239: RFI2; NbExp=3; IntAct=EBI-4446727, EBI-4425094;
CC Q94ID6; Q9S7W5: TCP13; NbExp=5; IntAct=EBI-4446727, EBI-4424877;
CC Q94ID6; Q9C9L2: TCP15; NbExp=6; IntAct=EBI-4446727, EBI-4426144;
CC Q94ID6; Q9LQF0: TCP23; NbExp=4; IntAct=EBI-4446727, EBI-15192297;
CC Q94ID6; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-4446727, EBI-25522447;
CC Q94ID6; Q9LVG2: TOE2; NbExp=4; IntAct=EBI-4446727, EBI-4424568;
CC Q94ID6; O64722: ZHD3; NbExp=3; IntAct=EBI-4446727, EBI-1806244;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC repression (EAR) motif, which may be involved in the activity of
CC transcriptional repression. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16760.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB055883; BAB62912.1; -; Genomic_DNA.
DR EMBL; AC010155; AAF16760.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30962.1; -; Genomic_DNA.
DR EMBL; AY099798; AAM20649.1; -; mRNA.
DR EMBL; BT002604; AAO00964.1; -; mRNA.
DR RefSeq; NP_174158.1; NM_102602.6.
DR AlphaFoldDB; Q94ID6; -.
DR SMR; Q94ID6; -.
DR BioGRID; 24967; 38.
DR IntAct; Q94ID6; 36.
DR STRING; 3702.AT1G28360.1; -.
DR PaxDb; Q94ID6; -.
DR PRIDE; Q94ID6; -.
DR EnsemblPlants; AT1G28360.1; AT1G28360.1; AT1G28360.
DR GeneID; 839732; -.
DR Gramene; AT1G28360.1; AT1G28360.1; AT1G28360.
DR KEGG; ath:AT1G28360; -.
DR Araport; AT1G28360; -.
DR TAIR; locus:2032510; AT1G28360.
DR eggNOG; ENOG502S0N7; Eukaryota.
DR HOGENOM; CLU_042594_5_2_1; -.
DR InParanoid; Q94ID6; -.
DR OMA; AHWASHS; -.
DR OrthoDB; 1557662at2759; -.
DR PhylomeDB; Q94ID6; -.
DR PRO; PR:Q94ID6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94ID6; baseline and differential.
DR Genevisible; Q94ID6; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; TAS:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..189
FT /note="Ethylene-responsive transcription factor 12"
FT /id="PRO_0000112562"
FT DNA_BIND 11..68
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 83..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 179..185
FT /note="EAR-like (transcriptional repression)"
FT COMPBIAS 83..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 189 AA; 20947 MW; FC58B8B7F656F0FF CRC64;
MASTTCAREV HYRGVRKRPW GRYAAEIRDP WKKTRVWLGT FDTPEEAALA YDGAARFLRG
IKAKTNFPSP LSLDLNHLPS APSAATAAAN NQPHQHQQLW FAAPPPVPPS SDHHHQHHRI
FLRTGVLNDK TSDYSSTEAP LYFTSSPNTA TSSPGYQVVG FPMMNSSPSP VTVRRGLAID
LNEPPPLWL
