AGR15_APLCA
ID AGR15_APLCA Reviewed; 156 AA.
AC P12285; P12284;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Neuroactive polyprotein R15;
DE Contains:
DE RecName: Full=R15 alpha-1 peptide;
DE Contains:
DE RecName: Full=R15 beta peptide;
DE Contains:
DE RecName: Full=R15 beta-f peptide;
DE Contains:
DE RecName: Full=R15 gamma peptide;
DE Flags: Precursor;
OS Aplysia californica (California sea hare).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC Aplysiidae; Aplysia.
OX NCBI_TaxID=6500;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RX PubMed=3652207; DOI=10.1016/0092-8674(87)90017-1;
RA Buck L.B., Bigelow J.M., Axel R.;
RT "Alternative splicing in individual Aplysia neurons generates neuropeptide
RT diversity.";
RL Cell 51:127-133(1987).
RN [2]
RP PARTIAL PROTEIN SEQUENCE, AND DISULFIDE BOND.
RX PubMed=2704753; DOI=10.1073/pnas.86.8.2913;
RA Weiss K.R., Bayley H., Lloyd P.E., Tenenbaum R., Kolks M.A.G., Buck L.B.,
RA Cropper E.C., Rosen S.C., Kupfermann I.;
RT "Purification and sequencing of neuropeptides contained in neuron R15 of
RT Aplysia californica.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:2913-2917(1989).
RN [3]
RP PYROGLUTAMATE FORMATION AT GLN-120.
RX PubMed=9930740; DOI=10.1046/j.1471-4159.1999.0720676.x;
RA Garden R.W., Moroz T.P., Gleeson J.M., Floyd P.D., Li L., Rubakhin S.S.,
RA Sweedler J.V.;
RT "Formation of N-pyroglutamyl peptides from N-Glu and N-Gln precursors in
RT Aplysia neurons.";
RL J. Neurochem. 72:676-681(1999).
RN [4]
RP TISSUE SPECIFICITY, DISULFIDE BOND, AND MASS SPECTROMETRY.
RX PubMed=17228083; DOI=10.1152/jn.00581.2006;
RA Romanova E.V., McKay N., Weiss K.R., Sweedler J.V., Koester J.;
RT "Autonomic control network active in Aplysia during locomotion includes
RT neurons that express splice variants of R15-neuropeptides.";
RL J. Neurophysiol. 97:481-491(2007).
CC -!- FUNCTION: The alpha-1 peptide acts as an osmoregulatory peptide,
CC increasing blood volume, and also modulates the activity of a set of
CC cardiac motor neurons that control heart rate.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced.;
CC Name=R15-2;
CC IsoId=P12285-1; Sequence=Displayed;
CC Name=R15-1;
CC IsoId=P12285-2; Sequence=VSP_029222;
CC -!- TISSUE SPECIFICITY: Expressed within the abdominal ganglion in neurons
CC R15, RB(HE), the two L9(G) gill motoneurons, and L40 interneuron, all
CC are parts of autonomic control circuit that contributes to implementing
CC a central command to coordinate autonomic activity with escape
CC locomotion. {ECO:0000269|PubMed:17228083}.
CC -!- MASS SPECTROMETRY: [R15 alpha-1 peptide]: Mass=3996.6; Mass_error=0.1;
CC Method=MALDI; Note=R15 alpha-1.;
CC Evidence={ECO:0000269|PubMed:17228083};
CC -!- MASS SPECTROMETRY: [R15 alpha-1 peptide]: Mass=2582.4; Mass_error=0.3;
CC Method=MALDI; Note=R15 alpha-2.;
CC Evidence={ECO:0000269|PubMed:17228083};
CC -!- MASS SPECTROMETRY: [R15 beta peptide]: Mass=2859.2; Mass_error=0.1;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:17228083};
CC -!- MASS SPECTROMETRY: [R15 beta-f peptide]: Mass=1833.0; Mass_error=0.0;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:17228083};
CC -!- MASS SPECTROMETRY: [R15 gamma peptide]: Mass=4497.0; Mass_error=0.1;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:17228083};
CC -!- MISCELLANEOUS: Isoform R15-1 produces peptide R15 alpha-2 which is a 24
CC aa long spliced form of peptide R15 alpha-1 which is produced by
CC isoform R15-2.
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DR EMBL; M17536; AAA27775.1; -; mRNA.
DR EMBL; M17535; AAA27774.1; -; mRNA.
DR RefSeq; XP_005096950.1; XM_005096893.2. [P12285-2]
DR AlphaFoldDB; P12285; -.
DR GeneID; 101859008; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Alternative splicing; Cleavage on pair of basic residues;
KW Direct protein sequencing; Disulfide bond; Neuropeptide;
KW Pyrrolidone carboxylic acid; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT PROPEP 27..48
FT /evidence="ECO:0000255"
FT /id="PRO_0000001796"
FT PEPTIDE 51..88
FT /note="R15 alpha-1 peptide"
FT /id="PRO_0000001797"
FT PEPTIDE 90..117
FT /note="R15 beta peptide"
FT /id="PRO_0000001798"
FT PEPTIDE 100..117
FT /note="R15 beta-f peptide"
FT /id="PRO_0000309495"
FT PEPTIDE 120..156
FT /note="R15 gamma peptide"
FT /id="PRO_0000001799"
FT MOD_RES 120
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:9930740"
FT DISULFID 74..81
FT /evidence="ECO:0000269|PubMed:17228083,
FT ECO:0000269|PubMed:2704753"
FT VAR_SEQ 53..68
FT /note="SDGSAERRPYTRMGSG -> YM (in isoform R15-1)"
FT /evidence="ECO:0000305"
FT /id="VSP_029222"
SQ SEQUENCE 156 AA; 17321 MW; 118BEAF69533D53E CRC64;
MDSAGLHINF RLSHVLTVVT CILYILPPTT TAYSLPAPGK AAFQHQLSKR DVSDGSAERR
PYTRMGSGGL KLHCQVHPAN CPGGLMVTKK SDLLGALLSR NSPSSYGLPS RDMSTAYKRQ
DVRQQLRMFD DLVQLRKLIE TPSVYPSEED EARLYD