ERG27_ASHGO
ID ERG27_ASHGO Reviewed; 348 AA.
AC Q74ZZ0;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=3-keto-steroid reductase;
DE EC=1.1.1.270;
GN Name=ERG27; OrderedLocusNames=AGR068W;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Responsible for the reduction of the keto group on the C-3 of
CC sterols. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxysteroid + NADP(+) = a 3-oxosteroid + H(+) +
CC NADPH; Xref=Rhea:RHEA:34787, ChEBI:CHEBI:15378, ChEBI:CHEBI:36836,
CC ChEBI:CHEBI:47788, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.270;
CC -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from
CC lanosterol: step 5/6.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. ERG27 subfamily. {ECO:0000305}.
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DR EMBL; AE016820; AAS54557.1; -; Genomic_DNA.
DR RefSeq; NP_986733.1; NM_211795.1.
DR AlphaFoldDB; Q74ZZ0; -.
DR SMR; Q74ZZ0; -.
DR STRING; 33169.AAS54557; -.
DR EnsemblFungi; AAS54557; AAS54557; AGOS_AGR068W.
DR GeneID; 4623034; -.
DR KEGG; ago:AGOS_AGR068W; -.
DR eggNOG; KOG1478; Eukaryota.
DR HOGENOM; CLU_029944_1_0_1; -.
DR InParanoid; Q74ZZ0; -.
DR OMA; ASYEGSK; -.
DR UniPathway; UPA00770; UER00758.
DR Proteomes; UP000000591; Chromosome VII.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005811; C:lipid droplet; IBA:GO_Central.
DR GO; GO:0000253; F:3-keto sterol reductase activity; IBA:GO_Central.
DR GO; GO:0102176; F:cycloeucalenone reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0006696; P:ergosterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase;
KW Reference proteome; Steroid biosynthesis.
FT CHAIN 1..348
FT /note="3-keto-steroid reductase"
FT /id="PRO_0000054589"
FT ACT_SITE 203
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 348 AA; 39684 MW; 74795DFA134E4B2D CRC64;
MLICHQCLSS RLTYISNLGL NIAYRLIEQF TDDSKLVIVV TSRTLPRVRE VVDLIKTYAE
KCGKSGAVDF DYLLVDFTDM VSVLGAAYEL EKRYDAIHYF YANAAQGVYS GIDWLGATKA
VLRDPLDAVT NPTYKIQRVG VKSRDGLGLV FQANVFGPYY LIRRIIPLLA KGKAKVVWLS
SLMSDVKYLS LEDVELLRTD SSYEGSKRLV DLLHLATYKE LKSLGIHQYV THPGIFTSHS
FYQYLNFFTY YGMLFLFYLA RWLGSPWHNI QGYKGANAPI YVATLANPTF EHQALKYGSA
TYRDGMEYIA KHEIDPTGMH DAYKYIRDLA EEWDIKLKDQ ITIGRSLS
