ID   ERG28_CAEEL             Reviewed;         162 AA.
AC   G5EEQ9;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Probable ergosterol biosynthetic protein 28 homolog {ECO:0000305};
GN   Name=erg-28 {ECO:0000303|PubMed:28168949, ECO:0000312|WormBase:C14C10.6};
GN   ORFNames=C14C10.6 {ECO:0000312|WormBase:C14C10.6};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP   AND MUTAGENESIS OF 156-GLN--LYS-162.
RX   PubMed=28168949; DOI=10.7554/elife.24733;
RA   Oh K.H., Haney J.J., Wang X., Chuang C.F., Richmond J.E., Kim H.;
RT   "ERG-28 controls BK channel trafficking in the ER to regulate synaptic
RT   function and alcohol response in C. elegans.";
RL   Elife 6:0-0(2017).
CC   -!- FUNCTION: Promotes the translocation of slo-1 potassium ion channels
CC       from the endoplasmic reticulum to its final destination at the plasma
CC       membrane, probably by shielding from premature proteasomal degradation
CC       in the endoplasmic reticulum. {ECO:0000269|PubMed:28168949}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:28168949}; Multi-pass membrane protein
CC       {ECO:0000255}. Cell projection, dendrite {ECO:0000269|PubMed:28168949}.
CC       Note=Localizes to puncta throughout synaptic terminals and dendrites.
CC       {ECO:0000269|PubMed:28168949}.
CC   -!- TISSUE SPECIFICITY: Expressed in tissues including muscles, intestine
CC       and neurons. {ECO:0000269|PubMed:28168949}.
CC   -!- DISRUPTION PHENOTYPE: Hypersensitive to the acetylcholinesterase
CC       inhibitor aldicarb. In a slo-1 (ky399gf) gain of function mutant,
CC       restores normal locomotory speed and sensitivity to aldicarb.
CC       {ECO:0000269|PubMed:28168949}.
CC   -!- SIMILARITY: Belongs to the ERG28 family. {ECO:0000305}.
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DR   EMBL; BX284605; CAC42259.1; -; Genomic_DNA.
DR   RefSeq; NP_506154.1; NM_073753.1.
DR   AlphaFoldDB; G5EEQ9; -.
DR   STRING; 6239.C14C10.6; -.
DR   EPD; G5EEQ9; -.
DR   PaxDb; G5EEQ9; -.
DR   PeptideAtlas; G5EEQ9; -.
DR   EnsemblMetazoa; C14C10.6.1; C14C10.6.1; WBGene00007589.
DR   GeneID; 179727; -.
DR   KEGG; cel:CELE_C14C10.6; -.
DR   CTD; 179727; -.
DR   WormBase; C14C10.6; CE28204; WBGene00007589; erg-28.
DR   eggNOG; KOG3455; Eukaryota.
DR   HOGENOM; CLU_1636954_0_0_1; -.
DR   InParanoid; G5EEQ9; -.
DR   OMA; QAMGSVW; -.
DR   OrthoDB; 1497130at2759; -.
DR   PRO; PR:G5EEQ9; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00007589; Expressed in embryo and 3 other tissues.
DR   GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cell projection; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..162
FT                   /note="Probable ergosterol biosynthetic protein 28 homolog"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000440094"
FT   TRANSMEM        7..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         156..162
FT                   /note="Missing: In cim16; restores the locomotory speed
FT                   defects and aldicarb sensitivity in the slo-1 (ky399gf)
FT                   gain of function mutant."
FT                   /evidence="ECO:0000269|PubMed:28168949"
SQ   SEQUENCE   162 AA;  18542 MW;  9A84DA3F21BD356D CRC64;
     MERSTRAWMS IVVVQAMGSV WMCYAKQNSA SHYTSTLPAL SRAHALPLAL LCILRIVLIF
     DFRNFSIHIA HILLSILTAI HTMTEVFFYQ SMSYGIVTVT EVTLNSFSVV VMLTFLLSPS
     FKNEQEGKEK RARKVTAKHY MEGEMLTPED DDELVQAYKK WK