AGR3_MOUSE
ID AGR3_MOUSE Reviewed; 165 AA.
AC Q8R3W7; A7NSI1; Q8BW95;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Anterior gradient protein 3 {ECO:0000312|MGI:MGI:2685734};
DE Flags: Precursor;
GN Name=Agr3 {ECO:0000312|MGI:MGI:2685734};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=25751668; DOI=10.1165/rcmb.2014-0318oc;
RA Bonser L.R., Schroeder B.W., Ostrin L.A., Baumlin N., Olson J.L.,
RA Salathe M., Erle D.J.;
RT "The ER resident protein AGR3 is required for regulation of ciliary beat
RT frequency in the airway.";
RL Am. J. Respir. Cell Mol. Biol. 53:536-543(2015).
CC -!- FUNCTION: Required for calcium-mediated regulation of ciliary beat
CC frequency and mucociliary clearance in the airway. Might be involved in
CC the regulation of intracellular calcium in tracheal epithelial cells.
CC {ECO:0000269|PubMed:25751668}.
CC -!- SUBUNIT: Interacts with LYPD3 and DAG1 (alphaDAG1).
CC {ECO:0000250|UniProtKB:Q8TD06}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q8TD06}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q8TD06}.
CC -!- TISSUE SPECIFICITY: Expressed in the ciliated cells of the airway
CC epithelium. Not detected in the mucous cells.
CC {ECO:0000269|PubMed:25751668}.
CC -!- DISRUPTION PHENOTYPE: Knockout mice develop ciliated cells with normal-
CC appearing cilia and histology. However, ciliary beat frequency was
CC lower in airways from knockout mice compared with control mice in
CC presence of calcium (20% lower in the absence of stimulation, 35% lower
CC after ATP stimulation). Knockout mice show reduced mucociliary
CC clearance. Knockout mice do not seem to show endoplasmic reticulum
CC stress response. {ECO:0000269|PubMed:25751668}.
CC -!- SIMILARITY: Belongs to the AGR family. {ECO:0000305}.
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DR EMBL; AK053177; BAC35297.1; -; mRNA.
DR EMBL; CT030196; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023499; AAH23499.1; -; mRNA.
DR CCDS; CCDS25880.1; -.
DR RefSeq; NP_997414.2; NM_207531.3.
DR AlphaFoldDB; Q8R3W7; -.
DR SMR; Q8R3W7; -.
DR STRING; 10090.ENSMUSP00000049212; -.
DR iPTMnet; Q8R3W7; -.
DR PhosphoSitePlus; Q8R3W7; -.
DR MaxQB; Q8R3W7; -.
DR PaxDb; Q8R3W7; -.
DR PeptideAtlas; Q8R3W7; -.
DR PRIDE; Q8R3W7; -.
DR ProteomicsDB; 285771; -.
DR Antibodypedia; 25286; 300 antibodies from 34 providers.
DR DNASU; 403205; -.
DR Ensembl; ENSMUST00000042101; ENSMUSP00000049212; ENSMUSG00000036231.
DR GeneID; 403205; -.
DR KEGG; mmu:403205; -.
DR UCSC; uc007njl.2; mouse.
DR CTD; 155465; -.
DR MGI; MGI:2685734; Agr3.
DR VEuPathDB; HostDB:ENSMUSG00000036231; -.
DR eggNOG; ENOG502R72A; Eukaryota.
DR GeneTree; ENSGT00530000063273; -.
DR HOGENOM; CLU_088048_1_0_1; -.
DR InParanoid; Q8R3W7; -.
DR OMA; FHAHKSN; -.
DR OrthoDB; 1382017at2759; -.
DR PhylomeDB; Q8R3W7; -.
DR TreeFam; TF321449; -.
DR BioGRID-ORCS; 403205; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Agr3; mouse.
DR PRO; PR:Q8R3W7; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8R3W7; protein.
DR Bgee; ENSMUSG00000036231; Expressed in saccule of membranous labyrinth and 46 other tissues.
DR ExpressionAtlas; Q8R3W7; baseline and differential.
DR Genevisible; Q8R3W7; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0002162; F:dystroglycan binding; ISO:MGI.
DR GO; GO:0060548; P:negative regulation of cell death; IBA:GO_Central.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endoplasmic reticulum; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..165
FT /note="Anterior gradient protein 3"
FT /id="PRO_0000001041"
FT MOTIF 162..165
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000305"
FT CONFLICT 90
FT /note="A -> E (in Ref. 1; BAC35297)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 165 AA; 19081 MW; 39FBC7DCB36E122C CRC64;
MLHSALALCL LLITVSSNLA IAIKKEKRPP QTLSRGWGDD ITWVQTYEEG LFHARKSNKP
LMVIHHLEDC QYCQALKKEF AKNEEIQEMA QNDFIMLNLM HETTDKNLSP DGQYVPRIMF
VDPSLTVRAD ITGRYSNRLY TYEPQDLPML VDNMKKALRL IQSEL
