ID   ERG2_MAGO7              Reviewed;         221 AA.
AC   P33281; A4QSA4; G4ML13;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=C-8 sterol isomerase;
DE            EC=5.-.-.-;
DE   AltName: Full=Delta-8--delta-7 sterol isomerase;
GN   Name=ERG2; ORFNames=MGG_08624;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8082205; DOI=10.1007/bf00351674;
RA   Keon J.P.R., James C.S., Court S., Baden-Daintree C., Bailey A.M.,
RA   Burden M., Hargreaves J.A.;
RT   "Isolation of the ERG2 gene, encoding sterol delta 8-->delta 7 isomerase,
RT   from the rice blast fungus Magnaporthe grisea and its expression in the
RT   maize smut pathogen Ustilago maydis.";
RL   Curr. Genet. 25:531-537(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
CC   -!- FUNCTION: Catalyzes the reaction which results in unsaturation at C-7
CC       in the B ring of sterols.
CC   -!- PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from
CC       zymosterol: step 2/5.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ERG2 family. {ECO:0000305}.
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DR   EMBL; Z22775; CAA80454.1; -; Genomic_DNA.
DR   EMBL; CM001231; EHA58440.1; -; Genomic_DNA.
DR   PIR; S44061; S33457.
DR   RefSeq; XP_003711052.1; XM_003711004.1.
DR   AlphaFoldDB; P33281; -.
DR   SMR; P33281; -.
DR   STRING; 318829.MGG_08624T0; -.
DR   EnsemblFungi; MGG_08624T0; MGG_08624T0; MGG_08624.
DR   GeneID; 2679059; -.
DR   KEGG; mgr:MGG_08624; -.
DR   VEuPathDB; FungiDB:MGG_08624; -.
DR   eggNOG; KOG4143; Eukaryota.
DR   HOGENOM; CLU_085469_0_0_1; -.
DR   InParanoid; P33281; -.
DR   OMA; LWATTRI; -.
DR   OrthoDB; 1285317at2759; -.
DR   UniPathway; UPA00768; UER00761.
DR   Proteomes; UP000009058; Chromosome 1.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR006716; ERG2_sigma1_rcpt-like.
DR   PANTHER; PTHR10868; PTHR10868; 1.
DR   Pfam; PF04622; ERG2_Sigma1R; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Isomerase; Lipid biosynthesis; Lipid metabolism;
KW   Membrane; Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..221
FT                   /note="C-8 sterol isomerase"
FT                   /id="PRO_0000087017"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   221 AA;  24721 MW;  8D8478A278E05DDD CRC64;
     MSSPISGFLR FVAVLLAVVS PLVYLAEQRL ESFYVFDHKH LHELSTQAIA QHGNNTRAIV
     GHIVDELRAR PETTKYISVQ EEWVFNNAGG AMGGMYIIHA SVTEYLIIFG TAIGTEGHTG
     RHTADDYFNI LTGEQWAYVP GEYEPEVYPA GSVHHLRRGT VKQYKMPEGC FALEYARGWI
     PPMLFFGFAD GLSSTLDFPT LWQTTVVTGR EMLGNLMLGK F