ID   ERG2_NEUCR              Reviewed;         256 AA.
AC   Q92254; Q7RVD2; Q9C2G3;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=C-8 sterol isomerase;
DE            EC=5.-.-.-;
DE   AltName: Full=Delta-8--delta-7 sterol isomerase;
GN   Name=erg-1; ORFNames=9G6.010, NCU04156;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RA   Gilbert J., Orbach M.J.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Catalyzes the reaction which results in unsaturation at C-7
CC       in the B ring of sterols.
CC   -!- PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from
CC       zymosterol: step 2/5.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ERG2 family. {ECO:0000305}.
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DR   EMBL; U59671; AAB09470.1; -; Genomic_DNA.
DR   EMBL; AL513463; CAC28749.1; -; Genomic_DNA.
DR   EMBL; CM002240; EAA32076.2; -; Genomic_DNA.
DR   PIR; T46871; T46871.
DR   RefSeq; XP_961312.2; XM_956219.3.
DR   AlphaFoldDB; Q92254; -.
DR   SMR; Q92254; -.
DR   STRING; 5141.EFNCRP00000003843; -.
DR   EnsemblFungi; EAA32076; EAA32076; NCU04156.
DR   GeneID; 3877419; -.
DR   KEGG; ncr:NCU04156; -.
DR   VEuPathDB; FungiDB:NCU04156; -.
DR   HOGENOM; CLU_085469_0_0_1; -.
DR   InParanoid; Q92254; -.
DR   OMA; LWATTRI; -.
DR   UniPathway; UPA00768; UER00761.
DR   Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006696; P:ergosterol biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR006716; ERG2_sigma1_rcpt-like.
DR   PANTHER; PTHR10868; PTHR10868; 1.
DR   Pfam; PF04622; ERG2_Sigma1R; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Isomerase; Lipid biosynthesis; Lipid metabolism;
KW   Membrane; Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..256
FT                   /note="C-8 sterol isomerase"
FT                   /id="PRO_0000087018"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..30
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        86
FT                   /note="A -> R (in Ref. 1; AAB09470)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        211
FT                   /note="A -> P (in Ref. 1; AAB09470)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   256 AA;  28246 MW;  1EE6548AB05EA59F CRC64;
     MPPKKQSSSG GNKPSGSGSS SGRSSSGSSC RCSCRCRCSI GGWLKFFAIL FALVAPIAYV
     LEQRLESFYV FDTEHLHDLS KRAISAHGND TKAIVKYIVD ELNDRNGVAP YVNNDEEWVF
     NNAGGAMGAM YIIHASITEY LIIFGTAIGT EGHTGRHTAD DYFHILTGTQ TAYVPGEYEP
     EVYPPGSVHH LVRGTVKQYR MPESCFALEY ARGWIPPMLF FGYADTLSST LDFPTLWRTS
     VITGREMISN LLKGKF