ID   ERG2_USTMA              Reviewed;         241 AA.
AC   P32360; A0A0D1CC59; Q4PD79;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=C-8 sterol isomerase;
DE            EC=5.-.-.-;
DE   AltName: Full=Delta-8--delta-7 sterol isomerase;
GN   Name=ERG2; ORFNames=UMAG_01934;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IMI 103761;
RA   Burden R.S., James C.S., Bailey A.M., Keon J.P., Croxon R., Bard M.,
RA   Hargreaves J.A.;
RT   "Isolation and characterization of the ERG2 gene, encoding delta 8-->delta
RT   7 sterol isomerase, from the maize smut pathogen Ustilago maydis.";
RL   Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reaction which results in unsaturation at C-7
CC       in the B ring of sterols.
CC   -!- PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from
CC       zymosterol: step 2/5.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ERG2 family. {ECO:0000305}.
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DR   EMBL; Z17311; CAA78959.1; -; Genomic_DNA.
DR   EMBL; CM003142; KIS70782.1; -; Genomic_DNA.
DR   PIR; S29697; S29697.
DR   RefSeq; XP_011387854.1; XM_011389552.1.
DR   AlphaFoldDB; P32360; -.
DR   SMR; P32360; -.
DR   STRING; 5270.UM01934P0; -.
DR   EnsemblFungi; KIS70782; KIS70782; UMAG_01934.
DR   GeneID; 23562800; -.
DR   KEGG; uma:UMAG_01934; -.
DR   VEuPathDB; FungiDB:UMAG_01934; -.
DR   eggNOG; KOG4143; Eukaryota.
DR   HOGENOM; CLU_085469_0_0_1; -.
DR   InParanoid; P32360; -.
DR   OMA; WKTTRIT; -.
DR   OrthoDB; 1285317at2759; -.
DR   UniPathway; UPA00768; UER00761.
DR   Proteomes; UP000000561; Chromosome 3.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006696; P:ergosterol biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR006716; ERG2_sigma1_rcpt-like.
DR   PANTHER; PTHR10868; PTHR10868; 1.
DR   Pfam; PF04622; ERG2_Sigma1R; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Isomerase; Lipid biosynthesis; Lipid metabolism;
KW   Membrane; Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..241
FT                   /note="C-8 sterol isomerase"
FT                   /id="PRO_0000087020"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   241 AA;  26265 MW;  6833F6E8EAD0299D CRC64;
     MASHRPRSNK AANGASTSPK RSWIIVSAAL VGFCALIAAL DSIRSSFYIF DHKAIYKIAS
     TAVANHPGNA TAIFDDVLDN LRADPKLAPY INKNHFSDES EWMFNNAGGA MGSMFIIHAS
     VTEYLIFFGT PVGTEGHTGR HTADDYFNIL TGNQYAFPAG ALKAEHYPAG SVHHLRRGTV
     KQYMMPEDGC WALELAQGWI PPMLPFGLAD VLSSTLDLPT FGITVWITAR EMVGNLLIGK
     F