ID   ERG3_MYCBO              Reviewed;         300 AA.
AC   P68434; A0A1R3XZE0; Q50619; X2BJ51;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=C-5 sterol desaturase;
DE            EC=1.3.-.-;
DE   AltName: Full=Sterol-C5-desaturase;
GN   Name=erg3; OrderedLocusNames=BQ2027_MB1844;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG;
RA   Jackson C.J., Lamb D.C., Kelly D.E., Kelly S.L.;
RT   "Characterization of a sterol delta 5,6-desaturase homolog in Mycobacterium
RT   bovis (BCG).";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF283500; AAG10160.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU00448.1; -; Genomic_DNA.
DR   RefSeq; NP_855496.1; NC_002945.3.
DR   RefSeq; WP_003901259.1; NC_002945.4.
DR   AlphaFoldDB; P68434; -.
DR   EnsemblBacteria; SIU00448; SIU00448; BQ2027_MB1844.
DR   PATRIC; fig|233413.5.peg.2025; -.
DR   OMA; HSSQHYN; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Oxidoreductase; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..300
FT                   /note="C-5 sterol desaturase"
FT                   /id="PRO_0000117040"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          94..227
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   300 AA;  34882 MW;  C5EB06B02E2B6A4A CRC64;
     MRDPVLFAIP CFLLLLILEW TAARKLESIE TAATGQPRPA SGAYLTRDSV ASISMGLVSI
     ATTAGWKSLA LLGYAAIYAY LAPWQLSAHR WYTWVIAIVG VDLLYYSYHR IAHRVRLIWA
     THQAHHSSEY FNFATALRQK WNNSGEILMW VPLPLMGLPP WMVFCSWSLN LIYQFWVHTE
     RIDRLPRWFE FVFNTPSHHR VHHGMDPVYL DKNYGGILII WDRLFGSFQP ELFRPHYGLT
     KRVDTFNIWK LQTREYVAIV RDWRSATRLR DRLGYVFGPP GWEPRTIDKS NAAASLVTSR