ID   ERG3_MYCTO              Reviewed;         300 AA.
AC   P9WNZ8; L0TAG8; P68435; Q50619;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=C-5 sterol desaturase;
DE            EC=1.3.-.-;
DE   AltName: Full=Sterol-C5-desaturase;
GN   Name=erg3; OrderedLocusNames=MT1862;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK46135.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK46135.1; ALT_INIT; Genomic_DNA.
DR   PIR; G70719; G70719.
DR   RefSeq; WP_003901259.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNZ8; -.
DR   EnsemblBacteria; AAK46135; AAK46135; MT1862.
DR   KEGG; mtc:MT1862; -.
DR   PATRIC; fig|83331.31.peg.2005; -.
DR   HOGENOM; CLU_033631_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   Oxidoreductase; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..300
FT                   /note="C-5 sterol desaturase"
FT                   /id="PRO_0000427039"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          94..227
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   300 AA;  34882 MW;  C5EB06B02E2B6A4A CRC64;
     MRDPVLFAIP CFLLLLILEW TAARKLESIE TAATGQPRPA SGAYLTRDSV ASISMGLVSI
     ATTAGWKSLA LLGYAAIYAY LAPWQLSAHR WYTWVIAIVG VDLLYYSYHR IAHRVRLIWA
     THQAHHSSEY FNFATALRQK WNNSGEILMW VPLPLMGLPP WMVFCSWSLN LIYQFWVHTE
     RIDRLPRWFE FVFNTPSHHR VHHGMDPVYL DKNYGGILII WDRLFGSFQP ELFRPHYGLT
     KRVDTFNIWK LQTREYVAIV RDWRSATRLR DRLGYVFGPP GWEPRTIDKS NAAASLVTSR