ID   ERG6_CLAL4              Reviewed;         375 AA.
AC   Q875K1; C4Y2B0;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Sterol 24-C-methyltransferase;
DE            EC=2.1.1.41;
DE   AltName: Full=Delta(24)-sterol C-methyltransferase;
GN   Name=ERG6; ORFNames=CLUG_02673;
OS   Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Metschnikowiaceae; Clavispora.
OX   NCBI_TaxID=306902;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12936965; DOI=10.1128/aac.47.9.2717-2724.2003;
RA   Young L.Y., Hull C.M., Heitman J.;
RT   "Disruption of ergosterol biosynthesis confers resistance to amphotericin B
RT   in Candida lusitaniae.";
RL   Antimicrob. Agents Chemother. 47:2717-2724(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42720;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the C-24 of zymosterol to form fecosterol. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + zymosterol = fecosterol + H(+) + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:21128, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17038, ChEBI:CHEBI:18252, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789; EC=2.1.1.41;
CC   -!- PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from
CC       zymosterol: step 1/5.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR   EMBL; AY179503; AAO21936.1; -; Genomic_DNA.
DR   EMBL; CH408078; EEQ38547.1; -; Genomic_DNA.
DR   RefSeq; XP_002617229.1; XM_002617183.1.
DR   AlphaFoldDB; Q875K1; -.
DR   SMR; Q875K1; -.
DR   STRING; 306902.Q875K1; -.
DR   PRIDE; Q875K1; -.
DR   EnsemblFungi; EEQ38547; EEQ38547; CLUG_02673.
DR   GeneID; 8497819; -.
DR   KEGG; clu:CLUG_02673; -.
DR   VEuPathDB; FungiDB:CLUG_02673; -.
DR   HOGENOM; CLU_039068_5_3_1; -.
DR   InParanoid; Q875K1; -.
DR   OMA; ISNMCKV; -.
DR   UniPathway; UPA00768; UER00760.
DR   Proteomes; UP000007703; Unassembled WGS sequence.
DR   GO; GO:0003838; F:sterol 24-C-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   3: Inferred from homology;
KW   Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT   CHAIN           1..375
FT                   /note="Sterol 24-C-methyltransferase"
FT                   /id="PRO_0000124791"
SQ   SEQUENCE   375 AA;  42698 MW;  4B652EAA78F7FBD7 CRC64;
     MTSVKLAEKN FEQDKEFAKA MHGDSYKKRG ISALMAKAKE ASDVATNGYF KHWDGGVSED
     DEKKRLDDYS SLTKNYYNLV TDFYEYGWGS SFHFSRYYKG EAFRQATARH EHFLAHKMNI
     NENMRVLDVG CGVGGPGREI CRFTDCTIVG LNNNDYQVER AQYYAKKYKL DDKLSYVKGD
     FMQMDFEPES FDAVYAIEAT VHAPVLEGVY SEIYKVLKPG GVFGVYEWVM TDKYDENNEE
     HRKIAYGIEV GDGIPKMYKR EVAEQALKNV GFDIEYEKDL ADVNDEIPWY YPLAGEWKYV
     QTLSDCFTIF RTSKIGRTVT TNVVGLMEKI GLAPKGSKQV TGALEDAAVN LVAGGEQKLF
     TPMMLYIARK PLDAK