ID   ERG7_PNECA              Reviewed;         719 AA.
AC   Q96WJ0;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Lanosterol synthase;
DE            EC=5.4.99.7;
DE   AltName: Full=2,3-epoxysqualene--lanosterol cyclase;
DE   AltName: Full=Oxidosqualene--lanosterol cyclase;
DE            Short=OSC;
GN   Name=ERG7;
OS   Pneumocystis carinii.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Pneumocystidomycetes; Pneumocystidaceae; Pneumocystis.
OX   NCBI_TaxID=4754;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12617471; DOI=10.1007/s11745-002-1017-9;
RA   Joubert B.M., LeClair R.J., Matsuda S.P.T., Balliano G.;
RT   "Subcellular localization of oxidosqualene cyclases from Arabidopsis
RT   thaliana, Trypanosoma cruzi, and Pneumocystis carinii expressed in yeast.";
RL   Lipids 37:1171-1176(2002).
CC   -!- FUNCTION: Catalyzes the cyclization of (S)-2,3 oxidosqualene to
CC       lanosterol, a reaction that forms the sterol nucleus.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = lanosterol; Xref=Rhea:RHEA:14621,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:16521; EC=5.4.99.7;
CC   -!- PATHWAY: Terpene metabolism; lanosterol biosynthesis; lanosterol from
CC       farnesyl diphosphate: step 3/3.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AF285825; AAK82993.1; -; mRNA.
DR   AlphaFoldDB; Q96WJ0; -.
DR   SMR; Q96WJ0; -.
DR   BindingDB; Q96WJ0; -.
DR   ChEMBL; CHEMBL1075204; -.
DR   UniPathway; UPA00767; UER00753.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0000250; F:lanosterol synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Lipid biosynthesis; Lipid metabolism; Repeat;
KW   Steroid biosynthesis.
FT   CHAIN           1..719
FT                   /note="Lanosterol synthase"
FT                   /id="PRO_0000072655"
FT   REPEAT          118..160
FT                   /note="PFTB 1"
FT   REPEAT          478..523
FT                   /note="PFTB 2"
FT   REPEAT          555..595
FT                   /note="PFTB 3"
FT   REPEAT          604..645
FT                   /note="PFTB 4"
FT   ACT_SITE        451
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   719 AA;  83308 MW;  4904DA1B8829F675 CRC64;
     MIYGYTEKEL EKTDPDGWRL IVEDTGRQRW KYLKTEEERR ERPQTYMEKY FLGKNMDLPE
     QPAAKTPIES ARKGFSFYKH LQTSDGNWAC EYGGVMFLLP GLIIAMYISK IEFPDEMRIE
     VIRYLVNHAN PEDGGWGIHI EGKSTVFGTA LNYVVLRILG LGPDHPVTMK ARIRLNELGG
     AIGCPQWGKF WLAVLNCYGW EGINPILPEF WMLPEWLPIH PSRWWVHTRA VYLPMGYIYG
     EKFTAPVDPL IESLREELYT QPYSSINFSK HRNTTSPVDV YVPHTRFLRV INSILTFYHT
     IFRFSWIKDM ASKYAYKLIE YENKNTDFLC IGPVNFSIHI LAVYWKEGPD SYAFKSHKER
     MADFLWISKK GMMMNGTNGV QLWDTSFAVQ ALVESGLAED PEFKDHMIKA LDFLDKCQIQ
     KNCDDQQKCY RHRRKGAWPF STRQQGYTVS DCTAEALKAV LLLQNLKSFP KRVSYDRLKD
     SVDVILSLQN KDGGFASYEL IRGPSWLEFI NPAEVFGDIM IEHSYPECTT AAVTALCYFR
     SLCSHYRGPE INKSVKNAIQ FIKESQRPDG SWYESWAICF TYATMFALES LSCVKDFYEN
     SFHSRRACDF LVNKQEEDGG WSEGYQSCTD GIWTRHPTGS QVVQTAWACI GLMYANYPDE
     TPIKRGINLI MSRQQPNGEW KQEAIEGVFN KNCMISYPNY KFNFTIKALG MYSKRYGNI