ERGI1_MOUSE
ID ERGI1_MOUSE Reviewed; 290 AA.
AC Q9DC16;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Endoplasmic reticulum-Golgi intermediate compartment protein 1;
DE AltName: Full=ER-Golgi intermediate compartment 32 kDa protein;
DE Short=ERGIC-32;
GN Name=Ergic1; Synonyms=Ergic32;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Eye, Lung, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and Czech II; TISSUE=Embryo, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 191-199, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Possible role in transport between endoplasmic reticulum and
CC Golgi. {ECO:0000250}.
CC -!- SUBUNIT: May form a heteromeric complex composed of ERGIC1, ERGIC2 and
CC ERGIC3 (By similarity). Within the complex, the interaction with ERGIC3
CC is direct (By similarity). Interacts with ERGIC3/ERV46 (By similarity).
CC {ECO:0000250|UniProtKB:Q969X5}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}. Endoplasmic reticulum-Golgi
CC intermediate compartment membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Note=Cycles between the
CC endoplasmic reticulum and the Golgi. {ECO:0000250}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ERGIC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK004629; BAB23423.1; -; mRNA.
DR EMBL; AK084385; BAC39170.1; -; mRNA.
DR EMBL; AK088157; BAC40179.1; -; mRNA.
DR EMBL; BC005516; AAH05516.1; -; mRNA.
DR EMBL; BC083144; AAH83144.1; -; mRNA.
DR CCDS; CCDS28553.1; -.
DR RefSeq; NP_080446.1; NM_026170.3.
DR AlphaFoldDB; Q9DC16; -.
DR SMR; Q9DC16; -.
DR BioGRID; 212202; 5.
DR IntAct; Q9DC16; 2.
DR STRING; 10090.ENSMUSP00000132922; -.
DR GlyGen; Q9DC16; 1 site.
DR iPTMnet; Q9DC16; -.
DR PhosphoSitePlus; Q9DC16; -.
DR SwissPalm; Q9DC16; -.
DR EPD; Q9DC16; -.
DR jPOST; Q9DC16; -.
DR MaxQB; Q9DC16; -.
DR PaxDb; Q9DC16; -.
DR PRIDE; Q9DC16; -.
DR ProteomicsDB; 275883; -.
DR TopDownProteomics; Q9DC16; -.
DR Antibodypedia; 17012; 82 antibodies from 17 providers.
DR Ensembl; ENSMUST00000167662; ENSMUSP00000132922; ENSMUSG00000001576.
DR GeneID; 67458; -.
DR KEGG; mmu:67458; -.
DR UCSC; uc008bef.1; mouse.
DR CTD; 57222; -.
DR MGI; MGI:1914708; Ergic1.
DR VEuPathDB; HostDB:ENSMUSG00000001576; -.
DR eggNOG; KOG2667; Eukaryota.
DR GeneTree; ENSGT00530000063113; -.
DR HOGENOM; CLU_034705_0_1_1; -.
DR InParanoid; Q9DC16; -.
DR OMA; SYQYTYA; -.
DR OrthoDB; 1318747at2759; -.
DR PhylomeDB; Q9DC16; -.
DR TreeFam; TF354253; -.
DR BioGRID-ORCS; 67458; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Ergic1; mouse.
DR PRO; PR:Q9DC16; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9DC16; protein.
DR Bgee; ENSMUSG00000001576; Expressed in ascending aorta and 259 other tissues.
DR ExpressionAtlas; Q9DC16; baseline and differential.
DR Genevisible; Q9DC16; MM.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; ISO:MGI.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISO:MGI.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR InterPro; IPR045888; Erv.
DR InterPro; IPR012936; Erv_C.
DR InterPro; IPR039542; Erv_N.
DR PANTHER; PTHR10984; PTHR10984; 1.
DR Pfam; PF07970; COPIIcoated_ERV; 1.
DR Pfam; PF13850; ERGIC_N; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport;
KW Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..290
FT /note="Endoplasmic reticulum-Golgi intermediate compartment
FT protein 1"
FT /id="PRO_0000087024"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..254
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 290 AA; 32562 MW; 9F6507BB1D89D46F CRC64;
MPFDFRRFDI YRKVPKDLTQ PTYTGAIISI CCCLFILFLF LSELTGFITT EVVNELYVDD
PDKDSGGKID VSLNISLPNL HCELVGLDIQ DEMGRHEVGH IDNSMKIPLN NGAGCRFEGQ
FSINKVPGNF HVSTHSATAQ PQNPDMTHTI HKLSFGDTLQ VQNVHGAFNA LGGADRLTSN
PLASHDYILK IVPTVYEDKS GKQRYSYQYT VANKEYVAYS HTGRIIPAIW FRYDLSPITV
KYTERRQPLY RFITTICAII GGTFTVAGIL DSCIFTASEA WKKIQLGKIH
