ERGI2_MOUSE
ID ERGI2_MOUSE Reviewed; 377 AA.
AC Q9CR89; B2KFC8; Q3UX13; Q9CWM6; Q9CYA2; Q9D4R1; Q9D8Z9;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Endoplasmic reticulum-Golgi intermediate compartment protein 2;
GN Name=Ergic2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RC TISSUE=Egg, Embryo, Lung, Placenta, Stomach, Testis, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, Czech II, and FVB/N; TISSUE=Egg, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Possible role in transport between endoplasmic reticulum and
CC Golgi. {ECO:0000250}.
CC -!- SUBUNIT: May form a heteromeric complex composed of ERGIC1, ERGIC2 and
CC ERGIC3 (By similarity). Interacts with ERGIC3, the interaction is
CC required for the stable expression of both proteins (By similarity).
CC May interact with EEF1A1 (By similarity).
CC {ECO:0000250|UniProtKB:Q96RQ1}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate
CC compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Endoplasmic
CC reticulum membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC Note=Cycles between the endoplasmic reticulum and the Golgi.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CR89-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CR89-2; Sequence=VSP_019211, VSP_019212;
CC -!- SIMILARITY: Belongs to the ERGIC family. {ECO:0000305}.
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DR EMBL; AK004661; BAB23451.1; -; mRNA.
DR EMBL; AK007498; BAB25070.1; -; mRNA.
DR EMBL; AK008971; BAB25998.1; -; mRNA.
DR EMBL; AK009483; BAB26318.1; -; mRNA.
DR EMBL; AK010529; BAB27008.1; -; mRNA.
DR EMBL; AK016275; BAB30175.1; -; mRNA.
DR EMBL; AK017876; BAB30984.1; -; mRNA.
DR EMBL; AK030045; BAC26758.1; -; mRNA.
DR EMBL; AK135968; BAE22750.1; -; mRNA.
DR EMBL; AK167391; BAE39481.1; -; mRNA.
DR EMBL; CU207318; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC006895; AAH06895.1; -; mRNA.
DR EMBL; BC018188; AAH18188.1; -; mRNA.
DR EMBL; BC026558; AAH26558.1; -; mRNA.
DR EMBL; BC064749; AAH64749.1; -; mRNA.
DR CCDS; CCDS20708.1; -. [Q9CR89-1]
DR RefSeq; NP_001273489.1; NM_001286560.1.
DR RefSeq; NP_080444.1; NM_026168.4. [Q9CR89-1]
DR RefSeq; XP_006507150.1; XM_006507087.2. [Q9CR89-1]
DR RefSeq; XP_017177205.1; XM_017321716.1. [Q9CR89-1]
DR RefSeq; XP_017177206.1; XM_017321717.1. [Q9CR89-2]
DR AlphaFoldDB; Q9CR89; -.
DR SMR; Q9CR89; -.
DR BioGRID; 212200; 2.
DR STRING; 10090.ENSMUSP00000120456; -.
DR GlyConnect; 2284; 1 N-Linked glycan (1 site).
DR iPTMnet; Q9CR89; -.
DR PhosphoSitePlus; Q9CR89; -.
DR SwissPalm; Q9CR89; -.
DR EPD; Q9CR89; -.
DR MaxQB; Q9CR89; -.
DR PaxDb; Q9CR89; -.
DR PeptideAtlas; Q9CR89; -.
DR PRIDE; Q9CR89; -.
DR ProteomicsDB; 275771; -. [Q9CR89-1]
DR ProteomicsDB; 275772; -. [Q9CR89-2]
DR Antibodypedia; 7205; 215 antibodies from 28 providers.
DR DNASU; 67456; -.
DR Ensembl; ENSMUST00000136008; ENSMUSP00000120456; ENSMUSG00000030304. [Q9CR89-1]
DR GeneID; 67456; -.
DR KEGG; mmu:67456; -.
DR UCSC; uc009etd.1; mouse. [Q9CR89-1]
DR UCSC; uc009etg.1; mouse. [Q9CR89-2]
DR CTD; 51290; -.
DR MGI; MGI:1914706; Ergic2.
DR VEuPathDB; HostDB:ENSMUSG00000030304; -.
DR eggNOG; KOG2667; Eukaryota.
DR GeneTree; ENSGT00530000063113; -.
DR HOGENOM; CLU_034705_4_1_1; -.
DR InParanoid; Q9CR89; -.
DR OMA; TYQYSVK; -.
DR OrthoDB; 1318747at2759; -.
DR PhylomeDB; Q9CR89; -.
DR TreeFam; TF317192; -.
DR BioGRID-ORCS; 67456; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Ergic2; mouse.
DR PRO; PR:Q9CR89; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9CR89; protein.
DR Bgee; ENSMUSG00000030304; Expressed in seminal vesicle and 269 other tissues.
DR ExpressionAtlas; Q9CR89; baseline and differential.
DR Genevisible; Q9CR89; MM.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; ISS:HGNC-UCL.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0061852; C:retrograte transporter complex, Golgi to ER; ISO:MGI.
DR GO; GO:1990351; C:transporter complex; ISO:MGI.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:HGNC-UCL.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; ISO:MGI.
DR InterPro; IPR045888; Erv.
DR InterPro; IPR012936; Erv_C.
DR InterPro; IPR039542; Erv_N.
DR PANTHER; PTHR10984; PTHR10984; 1.
DR Pfam; PF07970; COPIIcoated_ERV; 1.
DR Pfam; PF13850; ERGIC_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Membrane; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..377
FT /note="Endoplasmic reticulum-Golgi intermediate compartment
FT protein 2"
FT /id="PRO_0000239384"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..319
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..377
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 276..302
FT /note="ERIINHAAGSHGVSGIFMKYDLSSLMV -> RSLSKIQWEKQKNAVCKVYCV
FT VAMTED (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019211"
FT VAR_SEQ 303..377
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019212"
FT CONFLICT 3
FT /note="R -> G (in Ref. 3; BAB25070)"
FT /evidence="ECO:0000305"
FT CONFLICT 220
FT /note="L -> C (in Ref. 3; BAB30984)"
FT /evidence="ECO:0000305"
FT CONFLICT 254
FT /note="V -> M (in Ref. 3; BAB30984)"
FT /evidence="ECO:0000305"
FT CONFLICT 277
FT /note="R -> S (in Ref. 3; BAB30984)"
FT /evidence="ECO:0000305"
FT CONFLICT 341
FT /note="E -> D (in Ref. 3; BAB27008)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 42482 MW; 0FF37E719401C824 CRC64;
MRRLNRRKTL SLVKELDAFP KVPDSYVETS ASGGTVSLIA FTTMALLTIM EFSVYQDTWM
KYEYEVDKDF SSKLRINIDI TVAMKCHYVG ADVLDLAETM VASADGLAYE PALFDLSPQQ
REWQRMLQLI QSRLQEEHSL QDVIFKSAFK SASTALPPRE DDSSLTPDAC RIHGHLYVNK
VAGNFHITVG KAIPHPRGHA HLAALVNHDS YNFSHRIDHL SFGELVPGII NPLDGTEKIA
VDHNQMFQYF ITVVPTKLHT YKISADTHQF SVTERERIIN HAAGSHGVSG IFMKYDLSSL
MVTVTEEHMP FWQFFVRLCG IIGGIFSTTG MLHGIGKFIV EIICCRFRLG SYKPVRSVPF
ADGHTDNHLP LLENNTH
