ERGI3_HUMAN
ID ERGI3_HUMAN Reviewed; 383 AA.
AC Q9Y282; Q5JWS3; Q6ZWP7; Q9H276; Q9P1L3;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Endoplasmic reticulum-Golgi intermediate compartment protein 3;
DE AltName: Full=Serologically defined breast cancer antigen NY-BR-84;
GN Name=ERGIC3; Synonyms=C20orf47, ERV46, SDBCAG84; ORFNames=CGI-54, PRO0989;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Mammary gland;
RX PubMed=12747765;
RA Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O.,
RA Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.;
RT "Humoral immunity to human breast cancer: antigen definition and
RT quantitative analysis of mRNA expression.";
RL Cancer Immun. 1:4-4(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Zhang J., Liu T., Ye M., Zhang Q., Fu G., Zhou J., Wu J., Shen Y., Yu M.,
RA Chen S., Mao M., Chen Z.;
RT "Human hypothetical 43.2 Kd protein.";
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-383 (ISOFORM 1).
RC TISSUE=Fetal liver;
RA Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J.,
RA Liu M., He F.;
RT "Functional prediction of the coding sequences of 121 new genes deduced by
RT analysis of cDNA clones from human fetal liver.";
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ERGIC1.
RX PubMed=15308636; DOI=10.1074/jbc.m406644200;
RA Breuza L., Halbeisen R., Jenoe P., Otte S., Barlowe C., Hong W.,
RA Hauri H.-P.;
RT "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC)
RT membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new
RT cycling protein that interacts with human Erv46.";
RL J. Biol. Chem. 279:47242-47253(2004).
RN [9]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-241.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [14]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
RN [15]
RP FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH ERGIC1 AND ERGIC2,
RP INTERACTION WITH MARCHF2; SERPINA1; ERGIC1; ERGIC2 AND HP, UBIQUITINATED,
RP AND MUTAGENESIS OF 1-MET--CYS-25; LYS-6; LYS-8; LYS-15 AND LYS-23.
RX PubMed=31142615; DOI=10.1074/jbc.ra119.007435;
RA Yoo W., Cho E.B., Kim S., Yoon J.B.;
RT "The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of
RT secretory proteins.";
RL J. Biol. Chem. 294:10900-10912(2019).
RN [16]
RP VARIANT [LARGE SCALE ANALYSIS] LYS-297.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Possible role in transport between endoplasmic reticulum and
CC Golgi. Positively regulates trafficking of the secretory proteins
CC SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615).
CC {ECO:0000269|PubMed:31142615}.
CC -!- SUBUNIT: Forms homodimers (PubMed:31142615). May form a heteromeric
CC complex composed of ERGIC1, ERGIC2 and ERGIC3 (PubMed:31142615). Within
CC the complex, the interaction with ERGIC1 is direct (PubMed:31142615).
CC Interacts with ERGIC1/ERGIC32 (PubMed:15308636, PubMed:31142615).
CC Interacts with ERGIC2, the interaction is required for the stable
CC expression of both proteins (PubMed:31142615). Interacts with MARCHF2
CC (PubMed:31142615). Interacts with alpha1-antitrypsin/SERPINA1 and
CC HP/haptoglobin (PubMed:31142615). {ECO:0000269|PubMed:15308636,
CC ECO:0000269|PubMed:31142615}.
CC -!- INTERACTION:
CC Q9Y282; O95870: ABHD16A; NbExp=3; IntAct=EBI-781551, EBI-348517;
CC Q9Y282; Q9NRZ5: AGPAT4; NbExp=3; IntAct=EBI-781551, EBI-1754287;
CC Q9Y282; Q6RW13-2: AGTRAP; NbExp=3; IntAct=EBI-781551, EBI-11522760;
CC Q9Y282; Q9NVV5-2: AIG1; NbExp=3; IntAct=EBI-781551, EBI-11957045;
CC Q9Y282; Q5BKT4: ALG10; NbExp=3; IntAct=EBI-781551, EBI-13064220;
CC Q9Y282; O75106: AOC2; NbExp=3; IntAct=EBI-781551, EBI-17685278;
CC Q9Y282; P02656: APOC3; NbExp=3; IntAct=EBI-781551, EBI-1220113;
CC Q9Y282; P05090: APOD; NbExp=3; IntAct=EBI-781551, EBI-715495;
CC Q9Y282; Q9BQE5: APOL2; NbExp=3; IntAct=EBI-781551, EBI-4290634;
CC Q9Y282; Q96PS8: AQP10; NbExp=3; IntAct=EBI-781551, EBI-12820279;
CC Q9Y282; P41181: AQP2; NbExp=3; IntAct=EBI-781551, EBI-12701138;
CC Q9Y282; Q92482: AQP3; NbExp=3; IntAct=EBI-781551, EBI-2808854;
CC Q9Y282; P07306: ASGR1; NbExp=3; IntAct=EBI-781551, EBI-1172335;
CC Q9Y282; P27449: ATP6V0C; NbExp=3; IntAct=EBI-781551, EBI-721179;
CC Q9Y282; Q07817: BCL2L1; NbExp=3; IntAct=EBI-781551, EBI-78035;
CC Q9Y282; Q92843: BCL2L2; NbExp=3; IntAct=EBI-781551, EBI-707714;
CC Q9Y282; O15155: BET1; NbExp=3; IntAct=EBI-781551, EBI-749204;
CC Q9Y282; Q13323: BIK; NbExp=3; IntAct=EBI-781551, EBI-700794;
CC Q9Y282; Q12983: BNIP3; NbExp=3; IntAct=EBI-781551, EBI-749464;
CC Q9Y282; Q8WVV5: BTN2A2; NbExp=3; IntAct=EBI-781551, EBI-8648738;
CC Q9Y282; P06681: C2; NbExp=3; IntAct=EBI-781551, EBI-2835920;
CC Q9Y282; O14523: C2CD2L; NbExp=3; IntAct=EBI-781551, EBI-12822627;
CC Q9Y282; Q8WVX3-2: C4orf3; NbExp=3; IntAct=EBI-781551, EBI-12003442;
CC Q9Y282; Q9P0B6: CCDC167; NbExp=3; IntAct=EBI-781551, EBI-9083477;
CC Q9Y282; Q9UJ71: CD207; NbExp=3; IntAct=EBI-781551, EBI-2873235;
CC Q9Y282; Q8IX05: CD302; NbExp=3; IntAct=EBI-781551, EBI-14259393;
CC Q9Y282; P60033: CD81; NbExp=3; IntAct=EBI-781551, EBI-712921;
CC Q9Y282; O14735: CDIPT; NbExp=3; IntAct=EBI-781551, EBI-358858;
CC Q9Y282; O95674: CDS2; NbExp=3; IntAct=EBI-781551, EBI-3913685;
CC Q9Y282; Q9BXR6: CFHR5; NbExp=3; IntAct=EBI-781551, EBI-11579371;
CC Q9Y282; Q9H5X1: CIAO2A; NbExp=3; IntAct=EBI-781551, EBI-752069;
CC Q9Y282; Q8N6F1-2: CLDN19; NbExp=3; IntAct=EBI-781551, EBI-12256978;
CC Q9Y282; P56748: CLDN8; NbExp=3; IntAct=EBI-781551, EBI-10215641;
CC Q9Y282; Q8NHS1: CLDND2; NbExp=3; IntAct=EBI-781551, EBI-11959453;
CC Q9Y282; Q96DZ9-2: CMTM5; NbExp=3; IntAct=EBI-781551, EBI-11522780;
CC Q9Y282; O95406: CNIH1; NbExp=3; IntAct=EBI-781551, EBI-12172273;
CC Q9Y282; Q8TBE1: CNIH3; NbExp=3; IntAct=EBI-781551, EBI-12208021;
CC Q9Y282; P29400-2: COL4A5; NbExp=3; IntAct=EBI-781551, EBI-12211159;
CC Q9Y282; Q5RI15: COX20; NbExp=3; IntAct=EBI-781551, EBI-2834035;
CC Q9Y282; Q4LDR2: CTXN3; NbExp=3; IntAct=EBI-781551, EBI-12019274;
CC Q9Y282; Q5JRM2: CXorf66; NbExp=3; IntAct=EBI-781551, EBI-12823659;
CC Q9Y282; P49447: CYB561; NbExp=3; IntAct=EBI-781551, EBI-8646596;
CC Q9Y282; O14569: CYB561D2; NbExp=3; IntAct=EBI-781551, EBI-717654;
CC Q9Y282; Q9BQA9: CYBC1; NbExp=3; IntAct=EBI-781551, EBI-2680384;
CC Q9Y282; P78329: CYP4F2; NbExp=3; IntAct=EBI-781551, EBI-1752413;
CC Q9Y282; P81534: DEFB103B; NbExp=3; IntAct=EBI-781551, EBI-12074168;
CC Q9Y282; Q15125: EBP; NbExp=3; IntAct=EBI-781551, EBI-3915253;
CC Q9Y282; Q08426: EHHADH; NbExp=3; IntAct=EBI-781551, EBI-2339219;
CC Q9Y282; Q9BV81: EMC6; NbExp=3; IntAct=EBI-781551, EBI-2820492;
CC Q9Y282; P54849: EMP1; NbExp=3; IntAct=EBI-781551, EBI-4319440;
CC Q9Y282; P54852: EMP3; NbExp=3; IntAct=EBI-781551, EBI-3907816;
CC Q9Y282; O75355-2: ENTPD3; NbExp=3; IntAct=EBI-781551, EBI-12279764;
CC Q9Y282; Q969X5: ERGIC1; NbExp=4; IntAct=EBI-781551, EBI-781527;
CC Q9Y282; Q96RQ1: ERGIC2; NbExp=2; IntAct=EBI-781551, EBI-4403663;
CC Q9Y282; Q92935: EXTL1; NbExp=3; IntAct=EBI-781551, EBI-1760167;
CC Q9Y282; Q7L5A8: FA2H; NbExp=3; IntAct=EBI-781551, EBI-11337888;
CC Q9Y282; Q92520: FAM3C; NbExp=3; IntAct=EBI-781551, EBI-2876774;
CC Q9Y282; Q96IV6: FAXDC2; NbExp=3; IntAct=EBI-781551, EBI-12142299;
CC Q9Y282; Q9UGM5: FETUB; NbExp=3; IntAct=EBI-781551, EBI-13049494;
CC Q9Y282; Q9Y3D6: FIS1; NbExp=3; IntAct=EBI-781551, EBI-3385283;
CC Q9Y282; Q14318: FKBP8; NbExp=3; IntAct=EBI-781551, EBI-724839;
CC Q9Y282; P54710-2: FXYD2; NbExp=3; IntAct=EBI-781551, EBI-13084584;
CC Q9Y282; Q14802-3: FXYD3; NbExp=3; IntAct=EBI-781551, EBI-12175685;
CC Q9Y282; Q9H0Q3: FXYD6; NbExp=3; IntAct=EBI-781551, EBI-713304;
CC Q9Y282; P01350: GAST; NbExp=3; IntAct=EBI-781551, EBI-3436637;
CC Q9Y282; Q8WWP7: GIMAP1; NbExp=3; IntAct=EBI-781551, EBI-11991950;
CC Q9Y282; Q96F15: GIMAP5; NbExp=3; IntAct=EBI-781551, EBI-6166686;
CC Q9Y282; P29033: GJB2; NbExp=3; IntAct=EBI-781551, EBI-3905204;
CC Q9Y282; Q13491-3: GPM6B; NbExp=3; IntAct=EBI-781551, EBI-11992640;
CC Q9Y282; Q8TDV0: GPR151; NbExp=3; IntAct=EBI-781551, EBI-11955647;
CC Q9Y282; P02724: GYPA; NbExp=3; IntAct=EBI-781551, EBI-702665;
CC Q9Y282; Q9HCP6: HHATL; NbExp=3; IntAct=EBI-781551, EBI-5916693;
CC Q9Y282; P09601: HMOX1; NbExp=3; IntAct=EBI-781551, EBI-2806151;
CC Q9Y282; P30519: HMOX2; NbExp=3; IntAct=EBI-781551, EBI-712096;
CC Q9Y282; P00738: HP; NbExp=2; IntAct=EBI-781551, EBI-1220767;
CC Q9Y282; Q01628: IFITM3; NbExp=3; IntAct=EBI-781551, EBI-7932862;
CC Q9Y282; P24593: IGFBP5; NbExp=3; IntAct=EBI-781551, EBI-720480;
CC Q9Y282; P11215: ITGAM; NbExp=3; IntAct=EBI-781551, EBI-2568251;
CC Q9Y282; Q8N5M9: JAGN1; NbExp=3; IntAct=EBI-781551, EBI-10266796;
CC Q9Y282; O00180: KCNK1; NbExp=3; IntAct=EBI-781551, EBI-3914675;
CC Q9Y282; Q8TAF8: LHFPL5; NbExp=3; IntAct=EBI-781551, EBI-2820517;
CC Q9Y282; Q9UBY5: LPAR3; NbExp=3; IntAct=EBI-781551, EBI-12033434;
CC Q9Y282; Q7L5N7: LPCAT2; NbExp=3; IntAct=EBI-781551, EBI-4280011;
CC Q9Y282; Q9P0N8: MARCHF2; NbExp=8; IntAct=EBI-781551, EBI-10317612;
CC Q9Y282; Q9NX47: MARCHF5; NbExp=3; IntAct=EBI-781551, EBI-2341610;
CC Q9Y282; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-781551, EBI-11956541;
CC Q9Y282; Q6ZSS7: MFSD6; NbExp=3; IntAct=EBI-781551, EBI-2858252;
CC Q9Y282; P30301: MIP; NbExp=3; IntAct=EBI-781551, EBI-8449636;
CC Q9Y282; Q5J8X5: MS4A13; NbExp=3; IntAct=EBI-781551, EBI-12070086;
CC Q9Y282; Q96S97: MYADM; NbExp=3; IntAct=EBI-781551, EBI-13301517;
CC Q9Y282; Q9HB07: MYG1; NbExp=3; IntAct=EBI-781551, EBI-709754;
CC Q9Y282; Q9ULP0-2: NDRG4; NbExp=3; IntAct=EBI-781551, EBI-11978907;
CC Q9Y282; O95139: NDUFB6; NbExp=3; IntAct=EBI-781551, EBI-1053340;
CC Q9Y282; Q99519: NEU1; NbExp=3; IntAct=EBI-781551, EBI-721517;
CC Q9Y282; Q9NZG7: NINJ2; NbExp=3; IntAct=EBI-781551, EBI-10317425;
CC Q9Y282; Q16617: NKG7; NbExp=3; IntAct=EBI-781551, EBI-3919611;
CC Q9Y282; Q8N912: NRAC; NbExp=3; IntAct=EBI-781551, EBI-12051377;
CC Q9Y282; Q8WWG1: NRG4; NbExp=3; IntAct=EBI-781551, EBI-8637292;
CC Q9Y282; Q8IXM6: NRM; NbExp=3; IntAct=EBI-781551, EBI-10262547;
CC Q9Y282; P42857: NSG1; NbExp=3; IntAct=EBI-781551, EBI-6380741;
CC Q9Y282; Q9P0S3: ORMDL1; NbExp=3; IntAct=EBI-781551, EBI-1054848;
CC Q9Y282; Q8N138: ORMDL3; NbExp=3; IntAct=EBI-781551, EBI-721750;
CC Q9Y282; Q9NXK6: PAQR5; NbExp=3; IntAct=EBI-781551, EBI-10316423;
CC Q9Y282; Q86WK9: PAQR7; NbExp=3; IntAct=EBI-781551, EBI-10694587;
CC Q9Y282; Q96HA9: PEX11G; NbExp=3; IntAct=EBI-781551, EBI-17284886;
CC Q9Y282; Q9Y5Y5: PEX16; NbExp=3; IntAct=EBI-781551, EBI-981985;
CC Q9Y282; Q9UHJ9-5: PGAP2; NbExp=3; IntAct=EBI-781551, EBI-12092917;
CC Q9Y282; Q9Y342: PLLP; NbExp=3; IntAct=EBI-781551, EBI-3919291;
CC Q9Y282; P60201-2: PLP1; NbExp=3; IntAct=EBI-781551, EBI-12188331;
CC Q9Y282; Q5VZY2: PLPP4; NbExp=3; IntAct=EBI-781551, EBI-10485931;
CC Q9Y282; Q01453: PMP22; NbExp=3; IntAct=EBI-781551, EBI-2845982;
CC Q9Y282; Q8WZA1: POMGNT1; NbExp=3; IntAct=EBI-781551, EBI-3912424;
CC Q9Y282; Q59EV6: PPGB; NbExp=3; IntAct=EBI-781551, EBI-14210385;
CC Q9Y282; Q96AA3: RFT1; NbExp=3; IntAct=EBI-781551, EBI-6269616;
CC Q9Y282; Q02094: RHAG; NbExp=3; IntAct=EBI-781551, EBI-14772355;
CC Q9Y282; Q5QGT7: RTP2; NbExp=3; IntAct=EBI-781551, EBI-10244780;
CC Q9Y282; Q96GQ5: RUSF1; NbExp=3; IntAct=EBI-781551, EBI-8636004;
CC Q9Y282; Q14108: SCARB2; NbExp=3; IntAct=EBI-781551, EBI-1564650;
CC Q9Y282; O00767: SCD; NbExp=3; IntAct=EBI-781551, EBI-2684237;
CC Q9Y282; O75396: SEC22B; NbExp=3; IntAct=EBI-781551, EBI-1058865;
CC Q9Y282; Q15436: SEC23A; NbExp=3; IntAct=EBI-781551, EBI-81088;
CC Q9Y282; Q9Y6D0: SELENOK; NbExp=3; IntAct=EBI-781551, EBI-9679163;
CC Q9Y282; Q8N6R1: SERP2; NbExp=3; IntAct=EBI-781551, EBI-749270;
CC Q9Y282; P01009: SERPINA1; NbExp=2; IntAct=EBI-781551, EBI-986224;
CC Q9Y282; Q8TD22: SFXN5; NbExp=3; IntAct=EBI-781551, EBI-17274136;
CC Q9Y282; Q8WWT9: SLC13A3; NbExp=3; IntAct=EBI-781551, EBI-12938720;
CC Q9Y282; P43005: SLC1A1; NbExp=3; IntAct=EBI-781551, EBI-745376;
CC Q9Y282; Q8IWU4: SLC30A8; NbExp=3; IntAct=EBI-781551, EBI-10262251;
CC Q9Y282; P78382: SLC35A1; NbExp=3; IntAct=EBI-781551, EBI-12870360;
CC Q9Y282; Q96G79: SLC35A4; NbExp=3; IntAct=EBI-781551, EBI-12363689;
CC Q9Y282; Q8TB61: SLC35B2; NbExp=3; IntAct=EBI-781551, EBI-1054782;
CC Q9Y282; Q969S0: SLC35B4; NbExp=3; IntAct=EBI-781551, EBI-10281213;
CC Q9Y282; Q9NVC3: SLC38A7; NbExp=3; IntAct=EBI-781551, EBI-10314552;
CC Q9Y282; Q8IVJ1: SLC41A1; NbExp=3; IntAct=EBI-781551, EBI-12266234;
CC Q9Y282; Q96JW4: SLC41A2; NbExp=3; IntAct=EBI-781551, EBI-10290130;
CC Q9Y282; Q6UXD7-2: SLC49A3; NbExp=3; IntAct=EBI-781551, EBI-17682849;
CC Q9Y282; Q0VAQ4: SMAGP; NbExp=3; IntAct=EBI-781551, EBI-10226799;
CC Q9Y282; Q9NRQ5: SMCO4; NbExp=3; IntAct=EBI-781551, EBI-8640191;
CC Q9Y282; B2RUZ4: SMIM1; NbExp=3; IntAct=EBI-781551, EBI-12188413;
CC Q9Y282; Q9BZL3: SMIM3; NbExp=3; IntAct=EBI-781551, EBI-741850;
CC Q9Y282; Q6UX34: SNORC; NbExp=3; IntAct=EBI-781551, EBI-11957067;
CC Q9Y282; P16150: SPN; NbExp=3; IntAct=EBI-781551, EBI-10049055;
CC Q9Y282; P02808: STATH; NbExp=3; IntAct=EBI-781551, EBI-738687;
CC Q9Y282; Q86Y82: STX12; NbExp=3; IntAct=EBI-781551, EBI-2691717;
CC Q9Y282; P61266: STX1B; NbExp=3; IntAct=EBI-781551, EBI-9071709;
CC Q9Y282; Q13277: STX3; NbExp=3; IntAct=EBI-781551, EBI-1394295;
CC Q9Y282; Q9UNK0: STX8; NbExp=3; IntAct=EBI-781551, EBI-727240;
CC Q9Y282; O43759-2: SYNGR1; NbExp=3; IntAct=EBI-781551, EBI-12187159;
CC Q9Y282; P57105: SYNJ2BP; NbExp=3; IntAct=EBI-781551, EBI-1049004;
CC Q9Y282; Q8N2H4: SYS1; NbExp=3; IntAct=EBI-781551, EBI-13075176;
CC Q9Y282; Q03518: TAP1; NbExp=3; IntAct=EBI-781551, EBI-747259;
CC Q9Y282; Q9NZ01: TECR; NbExp=3; IntAct=EBI-781551, EBI-2877718;
CC Q9Y282; P02787: TF; NbExp=3; IntAct=EBI-781551, EBI-714319;
CC Q9Y282; P07204: THBD; NbExp=3; IntAct=EBI-781551, EBI-941422;
CC Q9Y282; P48230: TM4SF4; NbExp=3; IntAct=EBI-781551, EBI-8650934;
CC Q9Y282; Q9NV29: TMEM100; NbExp=3; IntAct=EBI-781551, EBI-8644968;
CC Q9Y282; Q6UX40: TMEM107; NbExp=3; IntAct=EBI-781551, EBI-12845616;
CC Q9Y282; Q9BVC6: TMEM109; NbExp=3; IntAct=EBI-781551, EBI-1057733;
CC Q9Y282; A0PK00: TMEM120B; NbExp=3; IntAct=EBI-781551, EBI-10171534;
CC Q9Y282; Q5BJH2-2: TMEM128; NbExp=3; IntAct=EBI-781551, EBI-10694905;
CC Q9Y282; Q96I45: TMEM141; NbExp=3; IntAct=EBI-781551, EBI-17681263;
CC Q9Y282; Q9Y6G1: TMEM14A; NbExp=3; IntAct=EBI-781551, EBI-2800360;
CC Q9Y282; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-781551, EBI-8638294;
CC Q9Y282; Q7Z7N9: TMEM179B; NbExp=3; IntAct=EBI-781551, EBI-11724423;
CC Q9Y282; Q969S6: TMEM203; NbExp=3; IntAct=EBI-781551, EBI-12274070;
CC Q9Y282; A2RU14: TMEM218; NbExp=3; IntAct=EBI-781551, EBI-10173151;
CC Q9Y282; Q9H0R3: TMEM222; NbExp=3; IntAct=EBI-781551, EBI-347385;
CC Q9Y282; Q8NBD8: TMEM229B; NbExp=3; IntAct=EBI-781551, EBI-12195227;
CC Q9Y282; Q9BU79: TMEM243; NbExp=3; IntAct=EBI-781551, EBI-12887458;
CC Q9Y282; Q69YG0: TMEM42; NbExp=3; IntAct=EBI-781551, EBI-12038591;
CC Q9Y282; Q9H2L4: TMEM60; NbExp=3; IntAct=EBI-781551, EBI-2852148;
CC Q9Y282; Q6PI78: TMEM65; NbExp=3; IntAct=EBI-781551, EBI-6656213;
CC Q9Y282; Q8N2M4: TMEM86A; NbExp=3; IntAct=EBI-781551, EBI-12015604;
CC Q9Y282; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-781551, EBI-2548832;
CC Q9Y282; Q5BJF2: TMEM97; NbExp=3; IntAct=EBI-781551, EBI-12111910;
CC Q9Y282; Q71RG4: TMUB2; NbExp=3; IntAct=EBI-781551, EBI-2820477;
CC Q9Y282; O14798: TNFRSF10C; NbExp=3; IntAct=EBI-781551, EBI-717441;
CC Q9Y282; Q96B49: TOMM6; NbExp=3; IntAct=EBI-781551, EBI-10826510;
CC Q9Y282; Q8N609: TRAM1L1; NbExp=3; IntAct=EBI-781551, EBI-11996766;
CC Q9Y282; Q13049: TRIM32; NbExp=3; IntAct=EBI-781551, EBI-742790;
CC Q9Y282; Q86UF1: TSPAN33; NbExp=3; IntAct=EBI-781551, EBI-12045841;
CC Q9Y282; Q5TGU0: TSPO2; NbExp=3; IntAct=EBI-781551, EBI-12195249;
CC Q9Y282; Q5BVD1: TTMP; NbExp=3; IntAct=EBI-781551, EBI-10243654;
CC Q9Y282; A5PKU2: TUSC5; NbExp=3; IntAct=EBI-781551, EBI-11988865;
CC Q9Y282; Q9Y385: UBE2J1; NbExp=3; IntAct=EBI-781551, EBI-988826;
CC Q9Y282; Q9Y5Z9: UBIAD1; NbExp=3; IntAct=EBI-781551, EBI-2819725;
CC Q9Y282; Q53HI1: UNC50; NbExp=3; IntAct=EBI-781551, EBI-7601760;
CC Q9Y282; Q9H1C4: UNC93B1; NbExp=3; IntAct=EBI-781551, EBI-4401271;
CC Q9Y282; P23763-3: VAMP1; NbExp=3; IntAct=EBI-781551, EBI-12097582;
CC Q9Y282; P63027: VAMP2; NbExp=3; IntAct=EBI-781551, EBI-520113;
CC Q9Y282; Q15836: VAMP3; NbExp=3; IntAct=EBI-781551, EBI-722343;
CC Q9Y282; O75379: VAMP4; NbExp=3; IntAct=EBI-781551, EBI-744953;
CC Q9Y282; O95183: VAMP5; NbExp=3; IntAct=EBI-781551, EBI-10191195;
CC Q9Y282; Q9BQB6: VKORC1; NbExp=3; IntAct=EBI-781551, EBI-6256462;
CC Q9Y282; Q8N0U8: VKORC1L1; NbExp=3; IntAct=EBI-781551, EBI-11337915;
CC Q9Y282; Q96GC9: VMP1; NbExp=3; IntAct=EBI-781551, EBI-2800296;
CC Q9Y282; Q6UX27-3: VSTM1; NbExp=3; IntAct=EBI-781551, EBI-12190699;
CC Q9Y282; Q9UEU0: VTI1B; NbExp=3; IntAct=EBI-781551, EBI-723716;
CC Q9Y282; O95070: YIF1A; NbExp=3; IntAct=EBI-781551, EBI-2799703;
CC Q9Y282; Q9Y548: YIPF1; NbExp=3; IntAct=EBI-781551, EBI-7850136;
CC Q9Y282; Q9BWQ6: YIPF2; NbExp=3; IntAct=EBI-781551, EBI-751204;
CC Q9Y282; Q9BSR8: YIPF4; NbExp=3; IntAct=EBI-781551, EBI-751253;
CC Q9Y282; Q96EC8: YIPF6; NbExp=3; IntAct=EBI-781551, EBI-751210;
CC Q9Y282; Q96MV8: ZDHHC15; NbExp=3; IntAct=EBI-781551, EBI-12837904;
CC Q9Y282; Q8IVQ6: ZDHHC21; NbExp=3; IntAct=EBI-781551, EBI-2849773;
CC Q9Y282; O95159: ZFPL1; NbExp=3; IntAct=EBI-781551, EBI-718439;
CC Q9Y282; Q96FB2; NbExp=3; IntAct=EBI-781551, EBI-2857623;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate
CC compartment membrane {ECO:0000269|PubMed:15308636}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:15308636}. Golgi apparatus, cis-Golgi
CC network membrane {ECO:0000269|PubMed:15308636}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:15308636}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:15308636}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15308636}. Note=Cycles between the endoplasmic
CC reticulum and the Golgi.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9Y282-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y282-2; Sequence=VSP_008652, VSP_008653;
CC Name=3;
CC IsoId=Q9Y282-3; Sequence=VSP_019208;
CC -!- SIMILARITY: Belongs to the ERGIC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF71038.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAG48265.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF308298; AAG48265.1; ALT_INIT; mRNA.
DR EMBL; AF151812; AAD34049.1; -; mRNA.
DR EMBL; AK074550; BAC11054.1; -; mRNA.
DR EMBL; AF077030; AAD27763.1; -; mRNA.
DR EMBL; AL121586; CAB89412.1; -; Genomic_DNA.
DR EMBL; AL121586; CAI42842.1; -; Genomic_DNA.
DR EMBL; BC009765; AAH09765.1; -; mRNA.
DR EMBL; BC014014; AAH14014.1; -; mRNA.
DR EMBL; AF116614; AAF71038.1; ALT_INIT; mRNA.
DR CCDS; CCDS13257.1; -. [Q9Y282-1]
DR CCDS; CCDS13258.1; -. [Q9Y282-3]
DR RefSeq; NP_057050.1; NM_015966.2. [Q9Y282-1]
DR RefSeq; NP_938408.1; NM_198398.1. [Q9Y282-3]
DR AlphaFoldDB; Q9Y282; -.
DR SMR; Q9Y282; -.
DR BioGRID; 119638; 345.
DR ComplexPortal; CPX-7221; ERGIC2-ERGIC3 retrograde receptor complex.
DR IntAct; Q9Y282; 259.
DR MINT; Q9Y282; -.
DR STRING; 9606.ENSP00000349970; -.
DR GlyGen; Q9Y282; 4 sites, 2 O-linked glycans (2 sites).
DR iPTMnet; Q9Y282; -.
DR PhosphoSitePlus; Q9Y282; -.
DR SwissPalm; Q9Y282; -.
DR BioMuta; ERGIC3; -.
DR DMDM; 37999823; -.
DR EPD; Q9Y282; -.
DR jPOST; Q9Y282; -.
DR MassIVE; Q9Y282; -.
DR MaxQB; Q9Y282; -.
DR PaxDb; Q9Y282; -.
DR PeptideAtlas; Q9Y282; -.
DR PRIDE; Q9Y282; -.
DR ProteomicsDB; 85687; -. [Q9Y282-1]
DR ProteomicsDB; 85688; -. [Q9Y282-2]
DR ProteomicsDB; 85689; -. [Q9Y282-3]
DR Antibodypedia; 3001; 256 antibodies from 31 providers.
DR DNASU; 51614; -.
DR Ensembl; ENST00000348547.7; ENSP00000341358.2; ENSG00000125991.21. [Q9Y282-1]
DR Ensembl; ENST00000357394.8; ENSP00000349970.4; ENSG00000125991.21. [Q9Y282-3]
DR GeneID; 51614; -.
DR KEGG; hsa:51614; -.
DR MANE-Select; ENST00000348547.7; ENSP00000341358.2; NM_015966.3; NP_057050.1.
DR UCSC; uc002xcs.4; human. [Q9Y282-1]
DR CTD; 51614; -.
DR DisGeNET; 51614; -.
DR GeneCards; ERGIC3; -.
DR HGNC; HGNC:15927; ERGIC3.
DR HPA; ENSG00000125991; Low tissue specificity.
DR neXtProt; NX_Q9Y282; -.
DR OpenTargets; ENSG00000125991; -.
DR PharmGKB; PA38050; -.
DR VEuPathDB; HostDB:ENSG00000125991; -.
DR eggNOG; KOG2667; Eukaryota.
DR GeneTree; ENSGT00530000063113; -.
DR InParanoid; Q9Y282; -.
DR OMA; IGNFHIA; -.
DR OrthoDB; 1318747at2759; -.
DR PhylomeDB; Q9Y282; -.
DR TreeFam; TF300739; -.
DR PathwayCommons; Q9Y282; -.
DR SignaLink; Q9Y282; -.
DR SIGNOR; Q9Y282; -.
DR BioGRID-ORCS; 51614; 32 hits in 1079 CRISPR screens.
DR ChiTaRS; ERGIC3; human.
DR GeneWiki; ERGIC3; -.
DR GenomeRNAi; 51614; -.
DR Pharos; Q9Y282; Tbio.
DR PRO; PR:Q9Y282; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q9Y282; protein.
DR Bgee; ENSG00000125991; Expressed in right uterine tube and 205 other tissues.
DR ExpressionAtlas; Q9Y282; baseline and differential.
DR Genevisible; Q9Y282; HS.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0061852; C:retrograte transporter complex, Golgi to ER; IPI:ComplexPortal.
DR GO; GO:1990351; C:transporter complex; IPI:ComplexPortal.
DR GO; GO:0043621; F:protein self-association; IPI:UniProtKB.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:ComplexPortal.
DR GO; GO:0090316; P:positive regulation of intracellular protein transport; IMP:UniProtKB.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR InterPro; IPR045888; Erv.
DR InterPro; IPR012936; Erv_C.
DR InterPro; IPR039542; Erv_N.
DR PANTHER; PTHR10984; PTHR10984; 1.
DR Pfam; PF07970; COPIIcoated_ERV; 1.
DR Pfam; PF13850; ERGIC_N; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Endoplasmic reticulum;
KW ER-Golgi transport; Glycoprotein; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..383
FT /note="Endoplasmic reticulum-Golgi intermediate compartment
FT protein 3"
FT /id="PRO_0000097640"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..341
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..383
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..25
FT /note="Required for MARCHF2-mediated degradation"
FT /evidence="ECO:0000269|PubMed:31142615"
FT SITE 8
FT /note="Ubiquitinated; by MARCHF2"
FT /evidence="ECO:0000269|PubMed:31142615"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378,
FT ECO:0007744|PubMed:25944712"
FT MOD_RES 116
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT CARBOHYD 266
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 229..230
FT /note="VH -> GE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12747765"
FT /id="VSP_008652"
FT VAR_SEQ 229
FT /note="V -> VHAVEI (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_019208"
FT VAR_SEQ 231..383
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12747765"
FT /id="VSP_008653"
FT VARIANT 113
FT /note="I -> L (in dbSNP:rs35505616)"
FT /id="VAR_048939"
FT VARIANT 297
FT /note="T -> K (in a colorectal cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036553"
FT MUTAGEN 1..25
FT /note="Missing: Abolishes MARCHF2-mediated degradation."
FT /evidence="ECO:0000269|PubMed:31142615"
FT MUTAGEN 6
FT /note="K->R: Reduces MARCHF2-mediated ubiquitination and
FT degradation. No effect on interaction with MARCHF2. Reduces
FT MARCHF2-mediated ubiquitination and degradation by 90%;
FT when associated with R-8."
FT /evidence="ECO:0000269|PubMed:31142615"
FT MUTAGEN 8
FT /note="K->R: Reduces MARCHF2-mediated ubiquitination and
FT degradation. No effect on interaction with MARCHF2. Reduces
FT MARCHF2-mediated ubiquitination and degradation by 90%;
FT when associated with R-8."
FT /evidence="ECO:0000269|PubMed:31142615"
FT MUTAGEN 15
FT /note="K->R: No effect on MARCHF2-mediated ubiquitination
FT and degradation."
FT /evidence="ECO:0000269|PubMed:31142615"
FT MUTAGEN 23
FT /note="K->R: No effect on MARCHF2-mediated ubiquitination
FT and degradation."
FT /evidence="ECO:0000269|PubMed:31142615"
FT CONFLICT 239
FT /note="N -> D (in Ref. 3; BAC11054)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 383 AA; 43222 MW; 8370FB3067AD95CE CRC64;
MEALGKLKQF DAYPKTLEDF RVKTCGGATV TIVSGLLMLL LFLSELQYYL TTEVHPELYV
DKSRGDKLKI NIDVLFPHMP CAYLSIDAMD VAGEQQLDVE HNLFKQRLDK DGIPVSSEAE
RHELGKVEVT VFDPDSLDPD RCESCYGAEA EDIKCCNTCE DVREAYRRRG WAFKNPDTIE
QCRREGFSQK MQEQKNEGCQ VYGFLEVNKV AGNFHFAPGK SFQQSHVHVH DLQSFGLDNI
NMTHYIQHLS FGEDYPGIVN PLDHTNVTAP QASMMFQYFV KVVPTVYMKV DGEVLRTNQF
SVTRHEKVAN GLLGDQGLPG VFVLYELSPM MVKLTEKHRS FTHFLTGVCA IIGGMFTVAG
LIDSLIYHSA RAIQKKIDLG KTT
