ERI3_HUMAN
ID ERI3_HUMAN Reviewed; 337 AA.
AC O43414; B1AK98; Q5T2T7; Q5T2T9; Q5TG35; Q9BQA0; Q9UEB4;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=ERI1 exoribonuclease 3;
DE EC=3.1.-.-;
DE AltName: Full=Prion interactor 1;
DE AltName: Full=Prion protein-interacting protein;
GN Name=ERI3; Synonyms=PINT1, PRNPIP, PRNPIP1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Yu W., Sarginson J., Gibbs R.A.;
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING
RP (ISOFORM 2).
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. {ECO:0000250};
CC -!- SUBUNIT: Interacts with PRNP. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O43414-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O43414-2; Sequence=VSP_031103;
CC Name=3;
CC IsoId=O43414-3; Sequence=VSP_031104;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC19158.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF007157; AAC19158.1; ALT_INIT; mRNA.
DR EMBL; AK290567; BAF83256.1; -; mRNA.
DR EMBL; AL035417; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL390776; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC004254; AAC04618.1; -; Genomic_DNA.
DR EMBL; BC001072; AAH01072.1; -; mRNA.
DR EMBL; BC004456; AAH04456.1; -; mRNA.
DR CCDS; CCDS30696.1; -. [O43414-1]
DR RefSeq; NP_001288627.1; NM_001301698.1.
DR RefSeq; NP_001288628.1; NM_001301699.1.
DR RefSeq; NP_001288629.1; NM_001301700.1.
DR RefSeq; NP_001288630.1; NM_001301701.1.
DR RefSeq; NP_076971.1; NM_024066.2. [O43414-1]
DR PDB; 2XRI; X-ray; 2.15 A; A=137-337.
DR PDB; 7K05; X-ray; 1.85 A; A/B=137-337.
DR PDB; 7K06; X-ray; 1.95 A; A/B=137-337.
DR PDB; 7K07; X-ray; 2.15 A; A/B=137-337.
DR PDB; 7LPY; X-ray; 1.85 A; A/B=137-337.
DR PDB; 7LPZ; X-ray; 1.55 A; A/B=137-337.
DR PDB; 7LQ0; X-ray; 1.60 A; A/B=137-337.
DR PDBsum; 2XRI; -.
DR PDBsum; 7K05; -.
DR PDBsum; 7K06; -.
DR PDBsum; 7K07; -.
DR PDBsum; 7LPY; -.
DR PDBsum; 7LPZ; -.
DR PDBsum; 7LQ0; -.
DR AlphaFoldDB; O43414; -.
DR SMR; O43414; -.
DR BioGRID; 122498; 24.
DR IntAct; O43414; 17.
DR MINT; O43414; -.
DR STRING; 9606.ENSP00000361331; -.
DR iPTMnet; O43414; -.
DR PhosphoSitePlus; O43414; -.
DR SwissPalm; O43414; -.
DR BioMuta; ERI3; -.
DR EPD; O43414; -.
DR jPOST; O43414; -.
DR MassIVE; O43414; -.
DR MaxQB; O43414; -.
DR PaxDb; O43414; -.
DR PeptideAtlas; O43414; -.
DR PRIDE; O43414; -.
DR ProteomicsDB; 48930; -. [O43414-1]
DR ProteomicsDB; 48931; -. [O43414-2]
DR ProteomicsDB; 48932; -. [O43414-3]
DR Antibodypedia; 18452; 81 antibodies from 18 providers.
DR DNASU; 79033; -.
DR Ensembl; ENST00000372257.7; ENSP00000361331.2; ENSG00000117419.16. [O43414-1]
DR Ensembl; ENST00000372259.9; ENSP00000361333.5; ENSG00000117419.16. [O43414-2]
DR GeneID; 79033; -.
DR KEGG; hsa:79033; -.
DR MANE-Select; ENST00000372257.7; ENSP00000361331.2; NM_024066.3; NP_076971.1.
DR UCSC; uc001clt.4; human. [O43414-1]
DR CTD; 79033; -.
DR GeneCards; ERI3; -.
DR HGNC; HGNC:17276; ERI3.
DR HPA; ENSG00000117419; Low tissue specificity.
DR MIM; 609917; gene.
DR neXtProt; NX_O43414; -.
DR OpenTargets; ENSG00000117419; -.
DR PharmGKB; PA164719272; -.
DR VEuPathDB; HostDB:ENSG00000117419; -.
DR eggNOG; KOG0542; Eukaryota.
DR GeneTree; ENSGT00530000063205; -.
DR HOGENOM; CLU_037266_0_0_1; -.
DR InParanoid; O43414; -.
DR OMA; YTWASYG; -.
DR PhylomeDB; O43414; -.
DR TreeFam; TF313449; -.
DR PathwayCommons; O43414; -.
DR SignaLink; O43414; -.
DR BioGRID-ORCS; 79033; 8 hits in 1075 CRISPR screens.
DR ChiTaRS; ERI3; human.
DR EvolutionaryTrace; O43414; -.
DR GenomeRNAi; 79033; -.
DR Pharos; O43414; Tbio.
DR PRO; PR:O43414; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; O43414; protein.
DR Bgee; ENSG00000117419; Expressed in left testis and 147 other tissues.
DR ExpressionAtlas; O43414; baseline and differential.
DR Genevisible; O43414; HS.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Exonuclease; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..337
FT /note="ERI1 exoribonuclease 3"
FT /id="PRO_0000317626"
FT DOMAIN 146..320
FT /note="Exonuclease"
FT ACT_SITE 152
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT ACT_SITE 307
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT BINDING 150
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 249
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 307
FT /ligand="AMP"
FT /ligand_id="ChEBI:CHEBI:456215"
FT /evidence="ECO:0000250"
FT BINDING 312
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..209
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031104"
FT VAR_SEQ 1..163
FT /note="MATASPAADGGRGRPWEGGLVSWPPAPPLTLPWTWMGPSWGQHPGHWGFPAL
FT TEPSASPAAGLGIFEVRRVLDASGCSMLAPLQTGAARFSSYLLSRARKVLGSHLFSPCG
FT VPEFCSISTRKLAAHGFGASMAAMVSFPPQRYHYFLVLDFEATCDKPQIHPQ -> MFV
FT MLQVPWLQSCANLVQIGAPLPLLGCLGQYFEVLGSMEVLAGQYGM (in isoform
FT 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_031103"
FT STRAND 144..149
FT /evidence="ECO:0007829|PDB:7K05"
FT STRAND 165..174
FT /evidence="ECO:0007829|PDB:7K05"
FT TURN 175..177
FT /evidence="ECO:0007829|PDB:7K05"
FT STRAND 180..187
FT /evidence="ECO:0007829|PDB:7K05"
FT STRAND 191..193
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 198..204
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 208..211
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 217..231
FT /evidence="ECO:0007829|PDB:7K05"
FT TURN 232..234
FT /evidence="ECO:0007829|PDB:7K05"
FT STRAND 240..247
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 248..251
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 253..261
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 267..270
FT /evidence="ECO:0007829|PDB:7K05"
FT STRAND 272..274
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 275..283
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 290..297
FT /evidence="ECO:0007829|PDB:7K05"
FT HELIX 309..325
FT /evidence="ECO:0007829|PDB:7K05"
SQ SEQUENCE 337 AA; 37238 MW; 28E8A691659B4D67 CRC64;
MATASPAADG GRGRPWEGGL VSWPPAPPLT LPWTWMGPSW GQHPGHWGFP ALTEPSASPA
AGLGIFEVRR VLDASGCSML APLQTGAARF SSYLLSRARK VLGSHLFSPC GVPEFCSIST
RKLAAHGFGA SMAAMVSFPP QRYHYFLVLD FEATCDKPQI HPQEIIEFPI LKLNGRTMEI
ESTFHMYVQP VVHPQLTPFC TELTGIIQAM VDGQPSLQQV LERVDEWMAK EGLLDPNVKS
IFVTCGDWDL KVMLPGQCQY LGLPVADYFK QWINLKKAYS FAMGCWPKNG LLDMNKGLSL
QHIGRPHSGI DDCKNIANIM KTLAYRGFIF KQTSKPF
