ID   ERIJ_HERER              Reviewed;         506 AA.
AC   A0A1V0QSE8;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=UDP-glycosyltransferase eriJ {ECO:0000303|PubMed:28371074};
DE            EC=2.4.1.- {ECO:0000269|PubMed:31535864};
DE   AltName: Full=Erinacine biosynthesis cluster protein J {ECO:0000303|PubMed:28371074};
GN   Name=eriJ {ECO:0000303|PubMed:28371074};
OS   Hericium erinaceus (Lion's mane mushroom) (Hydnum erinaceus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Russulales; Hericiaceae; Hericium.
OX   NCBI_TaxID=91752;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX   PubMed=28371074; DOI=10.1002/anie.201700565;
RA   Yang Y.L., Zhang S., Ma K., Xu Y., Tao Q., Chen Y., Chen J., Guo S.,
RA   Ren J., Wang W., Tao Y., Yin W.B., Liu H.;
RT   "Discovery and characterization of a new family of diterpene cyclases in
RT   bacteria and fungi.";
RL   Angew. Chem. Int. Ed. 56:4749-4752(2017).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=31535864; DOI=10.1021/jacs.9b08935;
RA   Liu C., Minami A., Ozaki T., Wu J., Kawagishi H., Maruyama J.I., Oikawa H.;
RT   "Efficient reconstitution of basidiomycota diterpene erinacine gene cluster
RT   in ascomycota host Aspergillus oryzae based on genomic DNA sequences.";
RL   J. Am. Chem. Soc. 141:15519-15523(2019).
CC   -!- FUNCTION: UDP-glycosyltransferase; part of the gene cluster that
CC       mediates the biosynthesis of erinacines, cyathane-xylosides that show
CC       unique biological activities, including leishmanicidal activity,
CC       stimulating activity for nerve growth-factor synthesis, and agonistic
CC       activity toward the kappa opioid receptor (PubMed:31535864). The
CC       geranylgeranyl diphosphate (GGPP) synthase eriE catalyzes the first
CC       step in erinacines biosynthesis via conversion of farnesyl
CC       pyrophosphate and isopentyl pyrophosphate into geranylgeranyl
CC       pyrophosphate (GGPP) (PubMed:31535864). GGPP is then substrate of the
CC       diterpene cyclase eriG for the production of cyatha-3,12-diene
CC       (PubMed:28371074). EriG is unable to use geranyl diphosphate (GPP) or
CC       farnesyl diphosphate (FPP) as substrates (PubMed:28371074). The
CC       cytochrome P450 monooxygenase eriI then hydroxylates cyatha-3,12-diene
CC       at C-14 of the seven-membered ring to produce erinacol, which is
CC       further hydroxylated at C-15 by the cytochrome P450 monooxygenase eriC
CC       to yield cyathadiol (PubMed:31535864). The cytochrome P450
CC       monooxygenase eriA then catalyzes C-11 hydroxylation in the presence of
CC       the short chain dehydrogenase/reductase (SDR) eriH, which leads to the
CC       production of cyathatriol (PubMed:31535864). The acetyltransferase eriL
CC       converts cyathatriol into 11-O-acetyl-cyathatriol (PubMed:31535864).
CC       The SDR eriH catalyzes further oxidation of 11-O-acetyl-cyathatriol
CC       into 1-O-acetylcyathin A3 (PubMed:31535864). Finally, the glycosyl
CC       transferase eriJ tranfers xylose from UDP-xylose onto C-14 of 11-O-
CC       acetyl-cyathatriol to form eracine Q (PubMed:31535864). EriJ is also
CC       able to convert 11-O-acetyl-cyathatriol to eracine Q2 by using UDP-D-
CC       glucose as cosubstrate, but at a lower rate (PubMed:31535864). In the
CC       absence of eriL and eriJ, the SDR eriH is able to convert cyathatriol
CC       to cyathin A3; this is likely a switching mechanism in the biosynthesis
CC       of cyathins (C-14 ketogroup)and erinacines (C-14 glycosylated group)
CC       (PubMed:31535864). The roles of the SDR eriB, the polyprenyl
CC       transferase eriF and the dehydrogenase eriK have still to be identified
CC       (Probable). {ECO:0000269|PubMed:28371074, ECO:0000269|PubMed:31535864,
CC       ECO:0000305|PubMed:31535864}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:31535864}.
CC   -!- INDUCTION: Expression is induced under erinacine P-producing
CC       conditions. {ECO:0000269|PubMed:28371074}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; KY683785; ARE72247.1; -; mRNA.
DR   AlphaFoldDB; A0A1V0QSE8; -.
DR   SMR; A0A1V0QSE8; -.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   Pfam; PF00201; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..506
FT                   /note="UDP-glycosyltransferase eriJ"
FT                   /id="PRO_0000452927"
SQ   SEQUENCE   506 AA;  55462 MW;  0682334B1891730E CRC64;
     MSTQNYHIVA VPPNEWGHMR PMIAFLARLV AVSLNSVDIT VTLIIAESAV AKARTELSIQ
     LAGEGIQSAE SRFEVVPTAV HMFWPADAYL PALKATYAEV IKKKEPDFAL LESMIHPFFD
     VVRSSATKPI KVGAWLPVAL PSWTSMAPIC YIRDDPQAYV KQVETTMAEK GLGYMEAASD
     AYLSHVNGRV LRIPGFPEMT DYEGFPQEPP VLLPVAMVVD WVFGIRDADI LVTSTAQALE
     RQGLQVFSKW LKEQPKHSDI LAVGPLTSQR TPEVARKEKE EADAGGFTAF LDAWAAKKGP
     KSVLYICFGS VLLPAEIEHL YAVMRVLLEL QIPFIMVLSD AARAALPADL AAAVRDSGLV
     KLTPWAPQQY ILAHAAVGWF LSHCGINGTL ESLCLRVPMV CWPLFADQPV LSILVAQVYG
     CGYELGEVRK GFGLKYRAST GKTPGGTVED VTREAREVFS KAFFNKAERA KVDANLEKMA
     TELNAAWDAE GDARASALAL LDFIRK