ERLI1_ARATH
ID ERLI1_ARATH Reviewed; 168 AA.
AC Q39176;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Lipid transfer protein EARLI 1;
DE AltName: Full=Protein EARLY ARABIDOPSIS ALUMINUM INDUCED 1;
DE Short=pEARLI1;
DE Flags: Precursor;
GN Name=EARLI1; OrderedLocusNames=At4g12480; ORFNames=T1P17.70;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY ALUMINUM, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RA Richards K.D., Gardner R.C.;
RT "pEARLI 1: an Arabidopsis member of a conserved gene family.";
RL (er) Plant Gene Register PGR95-099(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY VERNALIZATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. No-0;
RX PubMed=11202439; DOI=10.1023/a:1026536724779;
RA Wilkosz R., Schlaeppi M.;
RT "A gene expression screen identifies EARLI1 as a novel vernalization-
RT responsive gene in Arabidopsis thaliana.";
RL Plant Mol. Biol. 44:777-787(2000).
RN [7]
RP FUNCTION.
RX PubMed=17257167; DOI=10.1111/j.1365-313x.2006.03017.x;
RA Chassot C., Nawrath C., Metraux J.-P.;
RT "Cuticular defects lead to full immunity to a major plant pathogen.";
RL Plant J. 49:972-980(2007).
RN [8]
RP FUNCTION, INDUCTION BY COLD, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION,
RP AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=17786468; DOI=10.1007/s00425-007-0611-2;
RA Zhang Y., Schlappi M.;
RT "Cold responsive EARLI1 type HyPRPs improve freezing survival of yeast
RT cells and form higher order complexes in plants.";
RL Planta 227:233-243(2007).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=21815977; DOI=10.1111/j.1438-8677.2010.00428.x;
RA Shi Y., Zhang X., Xu Z.Y., Li L., Zhang C., Schlappi M., Xu Z.Q.;
RT "Influence of EARLI1-like genes on flowering time and lignin synthesis of
RT Arabidopsis thaliana.";
RL Plant Biol. 13:731-739(2011).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION BY COLD AND SALT.
RC STRAIN=cv. Columbia;
RX PubMed=21556912; DOI=10.1007/s00425-011-1425-9;
RA Xu D., Huang X., Xu Z.Q., Schlappi M.;
RT "The HyPRP gene EARLI1 has an auxiliary role for germinability and early
RT seedling development under low temperature and salt stress conditions in
RT Arabidopsis thaliana.";
RL Planta 234:565-577(2011).
RN [11]
RP FUNCTION AS ANTI-FUNGAL PROTEIN.
RC STRAIN=cv. Columbia;
RX PubMed=22759515; DOI=10.1016/j.gene.2012.06.070;
RA Li L., Zhang C., Xu D., Schlappi M., Xu Z.Q.;
RT "Expression of recombinant EARLI1, a hybrid proline-rich protein of
RT Arabidopsis, in Escherichia coli and its inhibition effect to the growth of
RT fungal pathogens and Saccharomyces cerevisiae.";
RL Gene 506:50-61(2012).
CC -!- FUNCTION: Probable lipid transfer protein (LTP). May improve freezing
CC survival. Seems to control the flowering process and lignin synthesis.
CC Has an auxiliary role for germinability and early seedling development
CC under low temperature and salt stress conditions, probably in an
CC abscisic acid- (ABA) dependent manner. Confers resistance to Botrytis
CC cinerea and exhibits anti-fungal activity, at least against
CC S.cerevisiae, B. cinerea and Fusarium oxysporum, probably by increasing
CC their membrane permeability. {ECO:0000269|PubMed:17257167,
CC ECO:0000269|PubMed:17786468, ECO:0000269|PubMed:21556912,
CC ECO:0000269|PubMed:21815977, ECO:0000269|PubMed:22759515}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:17786468}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in aerial part of seedlings, and,
CC to a lower extent, in roots. Higher basal levels in early-flowering
CC ecotypes. {ECO:0000269|PubMed:11202439, ECO:0000269|Ref.1}.
CC -!- DEVELOPMENTAL STAGE: Induced during germination in embryonic tissues,
CC and strongly expressed in certain parts of young seedlings, in the tips
CC of cotyledons and to a certain degree in developing leaves and roots.
CC {ECO:0000269|PubMed:21556912}.
CC -!- INDUCTION: Transient accumulation in response to toxic levels of
CC aluminum (Al). Stably activated by vernalization; vernalization and
CC subsequent growth in long-day photoperiods have an additive or
CC synergistic effect on this activation. Induced by both cold and salt.
CC {ECO:0000269|PubMed:11202439, ECO:0000269|PubMed:17786468,
CC ECO:0000269|PubMed:21556912, ECO:0000269|Ref.1}.
CC -!- DISRUPTION PHENOTYPE: Increased tendency for freezing-induced cellular
CC damage. Reduced cutin accumulation due to lower cutin biosynthesis.
CC Early flowering in long-day conditions. {ECO:0000269|PubMed:17786468,
CC ECO:0000269|PubMed:21815977}.
CC -!- SIMILARITY: Belongs to the plant LTP family. PEARLI1 subfamily.
CC {ECO:0000305}.
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DR EMBL; L43080; AAC37471.1; -; mRNA.
DR EMBL; AL049730; CAB41718.1; -; Genomic_DNA.
DR EMBL; AL161534; CAB78291.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83138.1; -; Genomic_DNA.
DR EMBL; AF412111; AAL06564.1; -; mRNA.
DR EMBL; AY133654; AAM91484.1; -; mRNA.
DR EMBL; AK230253; BAF02055.1; -; mRNA.
DR PIR; T07640; T07640.
DR RefSeq; NP_192985.1; NM_117318.3.
DR AlphaFoldDB; Q39176; -.
DR SMR; Q39176; -.
DR STRING; 3702.AT4G12480.1; -.
DR PaxDb; Q39176; -.
DR PRIDE; Q39176; -.
DR ProteomicsDB; 220695; -.
DR EnsemblPlants; AT4G12480.1; AT4G12480.1; AT4G12480.
DR GeneID; 826860; -.
DR Gramene; AT4G12480.1; AT4G12480.1; AT4G12480.
DR KEGG; ath:AT4G12480; -.
DR Araport; AT4G12480; -.
DR TAIR; locus:2135610; AT4G12480.
DR eggNOG; ENOG502S36E; Eukaryota.
DR HOGENOM; CLU_055715_3_2_1; -.
DR InParanoid; Q39176; -.
DR OMA; NVCNRKV; -.
DR OrthoDB; 1608612at2759; -.
DR PhylomeDB; Q39176; -.
DR PRO; PR:Q39176; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q39176; baseline and differential.
DR Genevisible; Q39176; AT.
DR GO; GO:0009707; C:chloroplast outer membrane; IDA:TAIR.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009505; C:plant-type cell wall; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0009682; P:induced systemic resistance; IMP:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0009409; P:response to cold; IMP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR CDD; cd01958; HPS_like; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR027923; Hydrophob_seed_dom.
DR Pfam; PF14547; Hydrophob_seed; 1.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Cell wall; Plant defense; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..168
FT /note="Lipid transfer protein EARLI 1"
FT /id="PRO_0000425604"
FT REPEAT 34..41
FT /note="A-1"
FT REPEAT 42..49
FT /note="A-2"
FT REPEAT 50..57
FT /note="A-3"
FT REPEAT 58..62
FT /note="B-1"
FT REPEAT 63..67
FT /note="B-2"
FT REGION 32..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..57
FT /note="3 X 8 AA repeats A of P-K-[HP]-K-P-V-P-S"
FT REGION 58..67
FT /note="2 X 58 AA tandem repeats B of P-S-V-P-S"
FT COMPBIAS 35..79
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 168 AA; 17308 MW; 752368B34493D9B7 CRC64;
MASKNSASIA LFFALNIIFF TLTAATDCGC NPSPKHKPVP SPKPKPVPSP KPKPVPSPSV
PSPSVPSPNP RPVTPPRTPG SSGNCPIDAL RLGVCANVLS SLLNIQLGQP SAQPCCSLIQ
GLVDLDAAIC LCTALRANVL GINLNVPISL SVLLNVCNRK VPSGFQCA
