ERN1_MEDTR
ID ERN1_MEDTR Reviewed; 268 AA.
AC A2Q5W1; A0A0C3WB51; A9Y0K7; G7KZH5;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Ethylene-responsive transcription factor ERN1 {ECO:0000305};
DE Short=ERF transcription factor ERN1 {ECO:0000305};
DE AltName: Full=NF box-binding protein 1 {ECO:0000303|PubMed:17827349};
DE AltName: Full=Protein ERF REQUIRED FOR NODULATION 1 {ECO:0000303|PubMed:17827349};
GN Name=ERN1 {ECO:0000303|PubMed:17827349};
GN Synonyms=ERN {ECO:0000303|PubMed:17449807},
GN NFbB1 {ECO:0000303|PubMed:17827349};
GN OrderedLocusNames=MTR_7g085810 {ECO:0000312|EMBL:AES80852.2};
GN ORFNames=MtrDRAFT_AC170580g4v1 {ECO:0000312|EMBL:ABN08981.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Jemalong A17;
RX PubMed=17449807; DOI=10.1105/tpc.106.048264;
RA Middleton P.H., Jakab J., Penmetsa R.V., Starker C.G., Doll J., Kalo P.,
RA Prabhu R., Marsh J.F., Mitra R.M., Kereszt A., Dudas B., Vandenbosch K.,
RA Long S.R., Cook D.R., Kiss G.B., Oldroyd G.E.;
RT "An ERF transcription factor in Medicago truncatula that is essential for
RT Nod factor signal transduction.";
RL Plant Cell 19:1221-1234(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION BY NOD FACTORS.
RX PubMed=17827349; DOI=10.1105/tpc.107.052944;
RA Andriankaja A., Boisson-Dernier A., Frances L., Sauviac L., Jauneau A.,
RA Barker D.G., de Carvalho-Niebel F.;
RT "AP2-ERF transcription factors mediate Nod factor dependent Mt ENOD11
RT activation in root hairs via a novel cis-regulatory motif.";
RL Plant Cell 19:2866-2885(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Town C.D.;
RT "Medicago truncatula chromosome 7 clone mte1-32m6 complete sequence.";
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23077241; DOI=10.1104/pp.112.203190;
RA Cerri M.R., Frances L., Laloum T., Auriac M.C., Niebel A., Oldroyd G.E.,
RA Barker D.G., Fournier J., de Carvalho-Niebel F.;
RT "Medicago truncatula ERN transcription factors: regulatory interplay with
RT NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial
RT infection.";
RL Plant Physiol. 160:2155-2172(2012).
RN [7]
RP FUNCTION.
RX PubMed=27208242; DOI=10.1104/pp.16.00230;
RA Cerri M.R., Frances L., Kelner A., Fournier J., Middleton P.H.,
RA Auriac M.C., Mysore K.S., Wen J., Erard M., Barker D.G., Oldroyd G.E.,
RA de Carvalho-Niebel F.;
RT "The symbiosis-related ERN transcription factors act in concert to
RT coordinate rhizobial host root infection.";
RL Plant Physiol. 171:1037-1054(2016).
CC -!- FUNCTION: Transcription factor involved in symbiotic nodule signaling
CC in response to rhizobial Nod factors (NFs) (PubMed:17449807,
CC PubMed:17827349). Binds to the GCC-box (NF-responsive box) of ENOD11
CC promoter. Acts as transcriptional activator of NF-responsive box-
CC containing target gene promoters in root hairs (PubMed:17827349).
CC Functions as a transcriptional regulator required for root infection by
CC symbiotic rhizobia, infection thread (IT) formation and maintenance,
CC and nodule development. Necessary for NF-induced gene expression and
CC spontaneous nodulation activated by CCAMK. Functions downstream of
CC CCAMK to activate nodulation gene expression (PubMed:17449807).
CC Involved in early stages of root nodule development. Functions
CC redundantly with ERN2. Is essential with ERN2 for the initiation of
CC root hair infection, and nodule organogenesis and development. Required
CC for accurate expression of the NF signaling genes ENOD11 and ENOD12
CC (PubMed:23077241, PubMed:27208242). {ECO:0000269|PubMed:17449807,
CC ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241,
CC ECO:0000269|PubMed:27208242}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366,
CC ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, root hairs and leaves
CC (PubMed:17827349). Expressed in root epidermis and root hairs
CC (PubMed:23077241). {ECO:0000269|PubMed:17827349,
CC ECO:0000269|PubMed:23077241}.
CC -!- INDUCTION: Induced in roots from 1 to 21 day after inoculation with
CC Sinorhizobium meliloti (PubMed:17449807). Induced by Nod factors in
CC root hairs (PubMed:17827349). {ECO:0000269|PubMed:17449807,
CC ECO:0000269|PubMed:17827349}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but roots of mutant plants, are impaired in the interaction
CC with rhizobia, and in infection thread (IT) initiation and maintenance.
CC {ECO:0000269|PubMed:17449807}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AES80852.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ984942; ABK28850.1; -; Genomic_DNA.
DR EMBL; EF396330; ABQ85894.1; -; Genomic_DNA.
DR EMBL; EU038802; ABW06102.2; -; mRNA.
DR EMBL; AC170580; ABN08981.1; -; Genomic_DNA.
DR EMBL; CM001223; AES80852.2; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_003624634.2; XM_003624586.2.
DR AlphaFoldDB; A2Q5W1; -.
DR SMR; A2Q5W1; -.
DR STRING; 3880.AES80852; -.
DR EnsemblPlants; AES80852; AES80852; MTR_7g085810.
DR GeneID; 11437275; -.
DR Gramene; AES80852; AES80852; MTR_7g085810.
DR KEGG; mtr:MTR_7g085810; -.
DR eggNOG; ENOG502QSNC; Eukaryota.
DR HOGENOM; CLU_056266_0_0_1; -.
DR OrthoDB; 1404076at2759; -.
DR Proteomes; UP000002051; Chromosome 7.
DR ExpressionAtlas; A2Q5W1; differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nodulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..268
FT /note="Ethylene-responsive transcription factor ERN1"
FT /id="PRO_0000444707"
FT DNA_BIND 38..95
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 268 AA; 29958 MW; DEE0679FF235F5D5 CRC64;
MEIQFQQPNM QNQKAGISVT NKGGKFKGRN RNSNNTNKFV GVRQRPSGRW VAEIKDTTQK
IRMWLGTFET AEEAARAYDE AACLLRGSNT RTNFITHVSL DSPLASRIRN LLNNRKGDKK
QEDGAVASAP SNSKTTISNT STITSNDDNK ESTLSTCATR NTELFEDAYK PDLSNCKEVF
ESGSQSNISC GFGPFFDHFS FTQLLDMAKN DDITDASSLE LSEFERMKVE RQISASLYAI
NGVHEYMETV QESNEALWDL PPLCSLFC
