ERN2_MEDTR
ID ERN2_MEDTR Reviewed; 313 AA.
AC A9Y0K8;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Ethylene-responsive transcription factor ERN2 {ECO:0000305};
DE Short=ERF transcription factor ERN2 {ECO:0000305};
DE AltName: Full=NF box-binding protein 2 {ECO:0000303|PubMed:17827349};
DE AltName: Full=Protein ERF REQUIRED FOR NODULATION 2 {ECO:0000303|PubMed:17827349};
GN Name=ERN2 {ECO:0000303|PubMed:17827349};
GN Synonyms=NFbB2 {ECO:0000303|PubMed:17827349};
GN OrderedLocusNames=MTR_6g029180 {ECO:0000312|EMBL:AES75163.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION BY NOD FACTORS.
RX PubMed=17827349; DOI=10.1105/tpc.107.052944;
RA Andriankaja A., Boisson-Dernier A., Frances L., Sauviac L., Jauneau A.,
RA Barker D.G., de Carvalho-Niebel F.;
RT "AP2-ERF transcription factors mediate Nod factor dependent Mt ENOD11
RT activation in root hairs via a novel cis-regulatory motif.";
RL Plant Cell 19:2866-2885(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23077241; DOI=10.1104/pp.112.203190;
RA Cerri M.R., Frances L., Laloum T., Auriac M.C., Niebel A., Oldroyd G.E.,
RA Barker D.G., Fournier J., de Carvalho-Niebel F.;
RT "Medicago truncatula ERN transcription factors: regulatory interplay with
RT NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial
RT infection.";
RL Plant Physiol. 160:2155-2172(2012).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF THR-61.
RX PubMed=27208242; DOI=10.1104/pp.16.00230;
RA Cerri M.R., Frances L., Kelner A., Fournier J., Middleton P.H.,
RA Auriac M.C., Mysore K.S., Wen J., Erard M., Barker D.G., Oldroyd G.E.,
RA de Carvalho-Niebel F.;
RT "The symbiosis-related ERN transcription factors act in concert to
RT coordinate rhizobial host root infection.";
RL Plant Physiol. 171:1037-1054(2016).
CC -!- FUNCTION: Transcription factor involved in symbiotic nodule signaling
CC in response to rhizobial Nod factors (NFs). Binds to the GCC box (NF-
CC responsive box) of ENOD11 promoter. Acts as transcriptional activator
CC of NF-responsive box-containing target gene promoters in root hairs
CC (PubMed:17827349). Involved in early stages of root nodule development.
CC Functions redundantly with ERN1. Is essential with ERN1 for the
CC initiation of root hair infection, and nodule organogenesis and
CC development. Required for accurate expression of the NF signaling genes
CC ENOD11 and ENOD12 (PubMed:23077241, PubMed:27208242).
CC {ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241,
CC ECO:0000269|PubMed:27208242}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366,
CC ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, root hairs and leaves
CC (PubMed:17827349). Expressed in root epidermis and root hairs
CC (PubMed:23077241). {ECO:0000269|PubMed:17827349,
CC ECO:0000269|PubMed:23077241}.
CC -!- INDUCTION: Induced by Nod factors in root hairs.
CC {ECO:0000269|PubMed:17827349}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; EU038803; ABW06103.2; -; mRNA.
DR EMBL; CM001222; AES75163.1; -; Genomic_DNA.
DR RefSeq; XP_003618945.1; XM_003618897.2.
DR AlphaFoldDB; A9Y0K8; -.
DR SMR; A9Y0K8; -.
DR STRING; 3880.AES75163; -.
DR EnsemblPlants; AES75163; AES75163; MTR_6g029180.
DR GeneID; 11417172; -.
DR Gramene; AES75163; AES75163; MTR_6g029180.
DR KEGG; mtr:MTR_6g029180; -.
DR eggNOG; ENOG502QSNC; Eukaryota.
DR HOGENOM; CLU_056266_0_0_1; -.
DR OMA; YRPDMRS; -.
DR OrthoDB; 1404076at2759; -.
DR Proteomes; UP000002051; Chromosome 6.
DR ExpressionAtlas; A9Y0K8; differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nodulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..313
FT /note="Ethylene-responsive transcription factor ERN2"
FT /id="PRO_0000444708"
FT DNA_BIND 32..89
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 108..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 61
FT /note="T->I: In ern2-1; reduced colonization of roots by
FT rhizobia, but still able to form nitrogen-fixing nodules
FT with premature senescence of the nodules."
FT /evidence="ECO:0000269|PubMed:27208242"
SQ SEQUENCE 313 AA; 34658 MW; 58E20D56570766B8 CRC64;
MEIQFDEPKK SLRPKKVNKF KGRNKKSETR DKFVGVRQRP SGRYVAEIKD TTQNIRMWLG
TFETAEEAAR AYDEAATLLR GSKTRTNFVT HVSYDSPLAS RIRHLLNNRK KGTKQQDMNG
ISSTTSHADT TNDTTSDGST SSTTNCIGTA SGAINSTSAS GVTSTSTNIS TSASGVASTS
TDISTNSSNT NVNDKSESLL SSSTTMQKPN LFEDAYRPDM SNLTNEYESS SYKSNVSWDF
GPIFDNFPFD QWLDMTNNDG LLCDMVDKGV SEFERMKVER QISASLYAIN GVQEYMKNVQ
DCNEAQWNLS PLC
