ID   ERN3_MEDTR              Reviewed;         238 AA.
AC   A9Y0K9;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Ethylene-responsive transcription factor ERN3 {ECO:0000305};
DE            Short=ERF transcription factor ERN3 {ECO:0000305};
DE   AltName: Full=NF box-binding protein 3 {ECO:0000303|PubMed:17827349};
DE   AltName: Full=Protein ERF REQUIRED FOR NODULATION 3 {ECO:0000303|PubMed:17827349};
GN   Name=ERN3 {ECO:0000303|PubMed:17827349};
GN   Synonyms=NFbB3 {ECO:0000303|PubMed:17827349};
GN   OrderedLocusNames=MTR_8g085960 {ECO:0000312|EMBL:AET04210.1};
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND INDUCTION BY NOD FACTORS.
RX   PubMed=17827349; DOI=10.1105/tpc.107.052944;
RA   Andriankaja A., Boisson-Dernier A., Frances L., Sauviac L., Jauneau A.,
RA   Barker D.G., de Carvalho-Niebel F.;
RT   "AP2-ERF transcription factors mediate Nod factor dependent Mt ENOD11
RT   activation in root hairs via a novel cis-regulatory motif.";
RL   Plant Cell 19:2866-2885(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=22089132; DOI=10.1038/nature10625;
RA   Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA   Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA   Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA   De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA   Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA   Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA   Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA   Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA   Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA   Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA   Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA   Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA   Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA   O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA   Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA   Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA   Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA   Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA   White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA   Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA   Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT   "The Medicago genome provides insight into the evolution of rhizobial
RT   symbioses.";
RL   Nature 480:520-524(2011).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Jemalong A17;
RX   PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA   Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA   Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA   Schwartz D.C., Town C.D.;
RT   "An improved genome release (version Mt4.0) for the model legume Medicago
RT   truncatula.";
RL   BMC Genomics 15:312-312(2014).
CC   -!- FUNCTION: Transcription factor involved in symbiotic nodule signaling
CC       in response to rhizobial Nod factors (NFs). Binds to the GCC box (NF-
CC       responsive box) of ENOD11 promoter. May act as transcriptional
CC       repressor of NF-responsive box-containing target gene promoters in root
CC       hairs. {ECO:0000269|PubMed:17827349}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366,
CC       ECO:0000269|PubMed:17827349}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, root hairs and leaves.
CC       {ECO:0000269|PubMed:17827349}.
CC   -!- INDUCTION: Induced by Nod factors in root hairs.
CC       {ECO:0000269|PubMed:17827349}.
CC   -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC       subfamily. {ECO:0000305}.
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DR   EMBL; EU038804; ABW06104.1; -; mRNA.
DR   EMBL; CM001224; AET04210.1; -; Genomic_DNA.
DR   RefSeq; XP_003629734.1; XM_003629686.2.
DR   AlphaFoldDB; A9Y0K9; -.
DR   SMR; A9Y0K9; -.
DR   STRING; 3880.AET04210; -.
DR   EnsemblPlants; AET04210; AET04210; MTR_8g085960.
DR   GeneID; 11408318; -.
DR   Gramene; AET04210; AET04210; MTR_8g085960.
DR   KEGG; mtr:MTR_8g085960; -.
DR   eggNOG; ENOG502RB9M; Eukaryota.
DR   HOGENOM; CLU_056266_0_0_1; -.
DR   OMA; YEYDVNY; -.
DR   OrthoDB; 1404076at2759; -.
DR   Proteomes; UP000002051; Chromosome 8.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR   CDD; cd00018; AP2; 1.
DR   Gene3D; 3.30.730.10; -; 1.
DR   InterPro; IPR001471; AP2/ERF_dom.
DR   InterPro; IPR036955; AP2/ERF_dom_sf.
DR   InterPro; IPR016177; DNA-bd_dom_sf.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   SMART; SM00380; AP2; 1.
DR   SUPFAM; SSF54171; SSF54171; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nodulation; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..238
FT                   /note="Ethylene-responsive transcription factor ERN3"
FT                   /id="PRO_0000444709"
FT   DNA_BIND        24..81
FT                   /note="AP2/ERF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
SQ   SEQUENCE   238 AA;  27199 MW;  5D768C3DDFB5B17D CRC64;
     MDYQNQKSNE NKHGEKLMMK NRSKFVGVRQ RASGKWAAEI KDTSKNIRMW LGTYKTAEEA
     ARAYDEAAFL LRGTNTRTNF STTHSIPTNS PISLKLKNLL HRKSISNLSQ SKNQCTLMSS
     SLQGAPIDNS IMVMENENKS SCSSEESKSL FWVQNQLVSE YNPYGVDMNM MNCSIGITPN
     TLQIDYSWPL PQQRINELPT LNDSVNVYGM NECYVEGTYE SKYEYDVNYP LSHLFCFT