ERP1_YEAST
ID ERP1_YEAST Reviewed; 219 AA.
AC Q05359; D6VPL7; E9P8S8;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Protein ERP1;
DE Flags: Precursor;
GN Name=ERP1; OrderedLocusNames=YAR002C-A; ORFNames=YAR002A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=7941740; DOI=10.1002/yea.320100413;
RA Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B.,
RA Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.;
RT "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42
RT kbp SPO7-CENI-CDC15 region.";
RL Yeast 10:535-541(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=10359606; DOI=10.1091/mbc.10.6.1923;
RA Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y.,
RA Bergeron J.J.M., Solari R.C., Rowley A.;
RT "Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.";
RL Mol. Biol. Cell 10:1923-1938(1999).
RN [6]
RP PROTEIN SEQUENCE OF 23-27, AND SUBCELLULAR LOCATION.
RX PubMed=10713261; DOI=10.1016/s0014-5793(00)01268-0;
RA Cho J.-H., Noda Y., Yoda K.;
RT "Proteins in the early Golgi compartment of Saccharomyces cerevisiae
RT immunoisolated by Sed5p.";
RL FEBS Lett. 469:151-154(2000).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Involved in vesicular protein trafficking.
CC -!- SUBUNIT: Associates with EMP24, ERV25 and ERP2.
CC -!- INTERACTION:
CC Q05359; Q12450: ERP4; NbExp=3; IntAct=EBI-6581, EBI-6598;
CC Q05359; P54837: ERV25; NbExp=5; IntAct=EBI-6581, EBI-6642;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:10713261}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:10713261}.
CC -!- MISCELLANEOUS: Present with 20700 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR EMBL; L22015; AAC04958.1; -; Genomic_DNA.
DR EMBL; AY557710; AAS56036.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA06987.1; -; Genomic_DNA.
DR PIR; S70311; S70311.
DR RefSeq; NP_009402.1; NM_001180033.1.
DR AlphaFoldDB; Q05359; -.
DR SMR; Q05359; -.
DR BioGRID; 31791; 172.
DR ComplexPortal; CPX-1698; EMP24 complex.
DR DIP; DIP-5147N; -.
DR IntAct; Q05359; 62.
DR MINT; Q05359; -.
DR STRING; 4932.YAR002C-A; -.
DR iPTMnet; Q05359; -.
DR MaxQB; Q05359; -.
DR PaxDb; Q05359; -.
DR PRIDE; Q05359; -.
DR EnsemblFungi; YAR002C-A_mRNA; YAR002C-A; YAR002C-A.
DR GeneID; 851264; -.
DR KEGG; sce:YAR002C-A; -.
DR SGD; S000002129; ERP1.
DR VEuPathDB; FungiDB:YAR002C-A; -.
DR eggNOG; KOG1690; Eukaryota.
DR GeneTree; ENSGT00940000175286; -.
DR HOGENOM; CLU_066963_2_1_1; -.
DR InParanoid; Q05359; -.
DR OMA; GVTCAWQ; -.
DR BioCyc; YEAST:G3O-28890-MON; -.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:Q05359; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; Q05359; protein.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IC:ComplexPortal.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0005798; C:Golgi-associated vesicle; IC:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006621; P:protein retention in ER lumen; IMP:SGD.
DR GO; GO:0006900; P:vesicle budding from membrane; IC:ComplexPortal.
DR InterPro; IPR015720; Emp24-like.
DR InterPro; IPR009038; GOLD_dom.
DR PANTHER; PTHR22811; PTHR22811; 1.
DR Pfam; PF01105; EMP24_GP25L; 1.
DR SMART; SM01190; EMP24_GP25L; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport;
KW Membrane; Protein transport; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:10713261"
FT CHAIN 23..219
FT /note="Protein ERP1"
FT /id="PRO_0000010408"
FT TOPO_DOM 23..186
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 32..131
FT /note="GOLD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
FT CONFLICT 152
FT /note="V -> I (in Ref. 4; AAS56036)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 219 AA; 24723 MW; 16236C1886FC42E8 CRC64;
MLLTSLLQVF ACCLVLPAQV TAFYYYTSGA ERKCFHKELS KGTLFQATYK AQIYDDQLQN
YRDAGAQDFG VLIDIEETFD DNHLVVHQKG SASGDLTFLA SDSGEHKICI QPEAGGWLIK
AKTKIDVEFQ VGSDEKLDSK GKATIDILHA KVNVLNSKIG EIRREQKLMR DREATFRDAS
EAVNSRAMWW IVIQLIVLAV TCGWQMKHLG KFFVKQKIL
