ERP4_YEAST
ID ERP4_YEAST Reviewed; 207 AA.
AC Q12450; D6W282;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Protein ERP4;
DE Flags: Precursor;
GN Name=ERP4; OrderedLocusNames=YOR016C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP GENE NAME.
RX PubMed=10359606; DOI=10.1091/mbc.10.6.1923;
RA Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y.,
RA Bergeron J.J.M., Solari R.C., Rowley A.;
RT "Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.";
RL Mol. Biol. Cell 10:1923-1938(1999).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in vesicular protein trafficking. {ECO:0000250}.
CC -!- INTERACTION:
CC Q12450; P32803: EMP24; NbExp=3; IntAct=EBI-6598, EBI-6431;
CC Q12450; Q05359: ERP1; NbExp=3; IntAct=EBI-6598, EBI-6581;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 2710 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the EMP24/GP25L family. {ECO:0000305}.
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DR EMBL; X87331; CAA60765.1; -; Genomic_DNA.
DR EMBL; Z74924; CAA99206.1; -; Genomic_DNA.
DR EMBL; AY693089; AAT93108.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10798.1; -; Genomic_DNA.
DR PIR; S54622; S54622.
DR RefSeq; NP_014659.1; NM_001183435.1.
DR AlphaFoldDB; Q12450; -.
DR SMR; Q12450; -.
DR BioGRID; 34420; 164.
DR DIP; DIP-7868N; -.
DR IntAct; Q12450; 29.
DR MINT; Q12450; -.
DR STRING; 4932.YOR016C; -.
DR MaxQB; Q12450; -.
DR PaxDb; Q12450; -.
DR PRIDE; Q12450; -.
DR EnsemblFungi; YOR016C_mRNA; YOR016C; YOR016C.
DR GeneID; 854181; -.
DR KEGG; sce:YOR016C; -.
DR SGD; S000005542; ERP4.
DR VEuPathDB; FungiDB:YOR016C; -.
DR eggNOG; KOG1693; Eukaryota.
DR GeneTree; ENSGT00940000166069; -.
DR HOGENOM; CLU_066963_4_2_1; -.
DR InParanoid; Q12450; -.
DR OMA; EICFDNG; -.
DR BioCyc; YEAST:G3O-33564-MON; -.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:Q12450; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12450; protein.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IGI:SGD.
DR GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006621; P:protein retention in ER lumen; IBA:GO_Central.
DR InterPro; IPR015720; Emp24-like.
DR InterPro; IPR009038; GOLD_dom.
DR InterPro; IPR036598; GOLD_dom_sf.
DR PANTHER; PTHR22811; PTHR22811; 1.
DR Pfam; PF01105; EMP24_GP25L; 1.
DR SMART; SM01190; EMP24_GP25L; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; ER-Golgi transport; Membrane; Protein transport;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..207
FT /note="Protein ERP4"
FT /id="PRO_0000010411"
FT TOPO_DOM 22..174
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 36..118
FT /note="GOLD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00096"
SQ SEQUENCE 207 AA; 23515 MW; 3C8FDC75577690D6 CRC64;
MRVFTLIAIL FSSSLLTHAF SSNYAPVGIS LPAFTKECLY YDLSSDKDVL VVSYQVLTGG
NFEIDFDITA PDGSVIVTER QKKHSDFLLK SFGIGKYTFC LSNNYGTSPK KVEITLEKEK
EIVSSHESKE DIIANNAIEE IDRNLNKITK TMDYLRAREW RNMYTVSSTE SRLTWLSLLI
MGVMVGISIV QALIIQFFFT SRQKNYV
