ERPA_HAEIN
ID ERPA_HAEIN Reviewed; 114 AA.
AC P45344;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Iron-sulfur cluster insertion protein ErpA {ECO:0000255|HAMAP-Rule:MF_01380};
GN Name=erpA {ECO:0000255|HAMAP-Rule:MF_01380}; OrderedLocusNames=HI_1723;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=10675023;
RX DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA Fountoulakis M.;
RT "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL Electrophoresis 21:411-429(2000).
RN [3]
RP STRUCTURE BY NMR.
RX PubMed=15014238; DOI=10.1023/b:jnmr.0000019242.88149.c5;
RA Yeh D.C., Liu F., Bonander N., Eisenstein E., Orban J.;
RT "NMR assignment of HI1723 from Haemophilus influenzae -- a sequence
RT homologue from the iron sulfur cluster assembly (IscA) family.";
RL J. Biomol. NMR 29:213-214(2004).
RN [4]
RP STRUCTURE BY NMR.
RG Structure 2 function project (S2F);
RT "Solution NMR structure of HI1723 from Haemophilus influenzae.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: Required for insertion of 4Fe-4S clusters for at least IspG.
CC {ECO:0000255|HAMAP-Rule:MF_01380}.
CC -!- COFACTOR:
CC Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01380};
CC Note=Binds 1 iron-sulfur cluster per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01380};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01380}.
CC -!- SIMILARITY: Belongs to the HesB/IscA family. {ECO:0000255|HAMAP-
CC Rule:MF_01380}.
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DR EMBL; L42023; AAC23369.1; -; Genomic_DNA.
DR PIR; F64176; F64176.
DR RefSeq; NP_439864.1; NC_000907.1.
DR RefSeq; WP_005649956.1; NC_000907.1.
DR PDB; 2APN; NMR; -; A=1-114.
DR PDBsum; 2APN; -.
DR AlphaFoldDB; P45344; -.
DR BMRB; P45344; -.
DR SMR; P45344; -.
DR STRING; 71421.HI_1723; -.
DR EnsemblBacteria; AAC23369; AAC23369; HI_1723.
DR KEGG; hin:HI_1723; -.
DR PATRIC; fig|71421.8.peg.1802; -.
DR eggNOG; COG0316; Bacteria.
DR HOGENOM; CLU_069054_5_3_6; -.
DR OMA; YQLYFDE; -.
DR PhylomeDB; P45344; -.
DR BioCyc; HINF71421:G1GJ1-1738-MON; -.
DR EvolutionaryTrace; P45344; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IBA:GO_Central.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR GO; GO:0106035; P:protein maturation by [4Fe-4S] cluster transfer; IBA:GO_Central.
DR Gene3D; 2.60.300.12; -; 1.
DR HAMAP; MF_01380; Fe_S_insert_ErpA; 1.
DR InterPro; IPR000361; FeS_biogenesis.
DR InterPro; IPR016092; FeS_cluster_insertion.
DR InterPro; IPR017870; FeS_cluster_insertion_CS.
DR InterPro; IPR023063; FeS_cluster_insertion_RrpA.
DR InterPro; IPR035903; HesB-like_dom_sf.
DR Pfam; PF01521; Fe-S_biosyn; 1.
DR SUPFAM; SSF89360; SSF89360; 1.
DR TIGRFAMs; TIGR00049; TIGR00049; 1.
DR PROSITE; PS01152; HESB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT CHAIN 1..114
FT /note="Iron-sulfur cluster insertion protein ErpA"
FT /id="PRO_0000076994"
FT BINDING 42
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01380"
FT BINDING 106
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01380"
FT BINDING 108
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01380"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:2APN"
FT HELIX 14..23
FT /evidence="ECO:0007829|PDB:2APN"
FT TURN 24..27
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 32..35
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:2APN"
FT HELIX 73..79
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:2APN"
FT STRAND 94..98
FT /evidence="ECO:0007829|PDB:2APN"
FT HELIX 100..103
FT /evidence="ECO:0007829|PDB:2APN"
SQ SEQUENCE 114 AA; 12194 MW; D57FB8986191BED0 CRC64;
MIDDMAVPLT FTDAAANKVK SLISEEENTD LKLRVYITGG GCSGFQYGFT FDEKVNDGDL
TIEKSGVQLV IDPMSLQYLI GGTVDYTEGL EGSRFTVNNP NATSTCGCGS SFSI
